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Mouse Anti-CNOT7 Recombinant Antibody (C4786) (V2LY-1206-LY475)


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Published Data
V2LY-1206-LY475-1
Mouse Anti-CNOT7 Recombinant Antibody (C4786) (V2LY-1206-LY475) in WB
U2OS cells transiently overexpressing Flag-tagged-CNOT6 were collected and lysed, and cell extracts used for anti-Flag immunoprecipitation. Representative immunoblots for the indicated proteins are shown. ...View More
Song, P., Liu, S., Liu, D., Keijzers, G., Bakula, D., Duan, S., ... & Rasmussen, L. J. (2022). CNOT6: A novel regulator of DNA mismatch repair. Cells, 11(3), 521.
Distributed under Open Access license CC BY 4.0, without modification.
V2LY-1206-LY475-2
Mouse Anti-CNOT7 Recombinant Antibody (C4786) (V2LY-1206-LY475) in WB and IP
The immunoprecipitated samples and inputs were resolved by SDS-PAGE and subjected to western blotting using the indicated antibodies. Tubulin was used as a loading control. ...View More
Masumura, Y., Higo, S., Asano, Y., Kato, H., Yan, Y., Ishino, S., ... & Sakata, Y. (2016). Btg2 is a negative regulator of cardiomyocyte hypertrophy through a decrease in cytosolic RNA. Scientific Reports, 6(1), 28592.
Distributed under Open Access license CC BY 4.0, without modification.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
C4786
Antibody Isotype
IgG2a, κ
Application
WB, IP, IF, ELISA

Basic Information

Immunogen
Amino acids 1-286 of CNOT7 of human.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
0.1% gelatin
Preservative
<0.1% sodium azide
Concentration
0.1 mg/ml
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
CCR4-NOT Transcription Complex Subunit 7
Entrez Gene ID
Human29883
Mouse18983
Rat306492
UniProt ID
HumanQ9UIV1
MouseQ60809
RatB3DMA5
Function
Has 3'-5' poly(A) exoribonuclease activity for synthetic poly(A) RNA substrate. Its function seems to be partially redundant with that of CNOT8. Catalytic component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. During miRNA-mediated repression the complex seems also to act as translational repressor during translational initiation. Additional complex functions may be a consequence of its influence on mRNA expression. Associates with members of the BTG family such as TOB1 and BTG2 and is required for their anti-proliferative activity.
Biological Process
Deadenylation-dependent decapping of nuclear-transcribed mRNA Source: Ensembl
Defense response to virus Source: UniProtKB
DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest Source: Reactome
Exonucleolytic catabolism of deadenylated mRNA Source: UniProtKB
Gene silencing by miRNA Source: UniProtKB
Gene silencing by RNA Source: UniProtKB
Negative regulation of cell population proliferation Source: UniProtKB
Negative regulation of gene expression Source: BHF-UCL
Negative regulation of transcription, DNA-templated Source: UniProtKB
Negative regulation of translation Source: GO_Central
Negative regulation of type I interferon-mediated signaling pathway Source: UniProtKB
Nuclear-transcribed mRNA poly(A) tail shortening Source: Reactome
P-body assembly Source: Ensembl
Positive regulation of cell population proliferation Source: UniProtKB
Positive regulation of mRNA catabolic process Source: UniProtKB
Positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay Source: UniProtKB
Positive regulation of nuclear-transcribed mRNA poly(A) tail shortening Source: UniProtKB
Positive regulation of transcription by RNA polymerase II Source: Ensembl
Positive regulation of viral genome replication Source: UniProtKB
Regulation of tyrosine phosphorylation of STAT protein Source: UniProtKB
Cellular Location
Nucleus; P-body. NANOS2 promotes its localization to P-body.
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For research use only. Not intended for any clinical use.

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