Mouse Anti-CNRIP1 Recombinant Antibody (CBXC-1795) (V2LY-1206-LY509)

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Basic Information

Host Animal
Mouse
Clone
CBXC-1795
Application
WB, IP, IF, ELISA
Immunogen
Amino acids 1-164 representing full length CNRIP1 of human.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, κ
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
0.1% gelatin
Preservative
<0.1% sodium azide
Concentration
0.2 mg/ml
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.
More Infomation

Target

Full Name
Cannabinoid Receptor Interacting Protein 1
Entrez Gene ID
Human25927
Mouse380686
Rat364208
UniProt ID
HumanQ96F85
MouseQ5M8N0
RatQ5M7A7
Function
Isoform 1:
Suppresses cannabinoid receptor CNR1-mediated tonic inhibition of voltage-gated calcium channels.
Isoform 2:
Does not suppress cannabinoid receptor CNR1-mediated tonic inhibition of voltage-gated calcium channels.
Biological Process
Negative regulation of signaling receptor activity Source: UniProtKB
Regulation of signaling receptor activity Source: UniProtKB
Cellular Location
Plasma membrane; Cytoplasm

Yang, F., Zhao, Y. J., Chen, S. J., Li, Y. R., Yang, P. Y., Qi, J. Y., ... & Zhang, K. (2022). Disrupting Cannabinoid Receptor Interacting Protein 1 Rescues Cognitive Flexibility in Long-Term Estrogen-Deprived Female Mice. Brain Research Bulletin.

Chen, D., Wu, H., Feng, X., Chen, Y., Lv, Z., Kota, V. G., ... & Wu, J. (2021). DNA Methylation of Cannabinoid Receptor Interacting Protein 1 Promotes Pathogenesis of Intrahepatic Cholangiocarcinoma Through Suppressing Parkin‐Dependent Pyruvate Kinase M2 Ubiquitination. Hepatology, 73(5), 1816-1835.

Oliver, E. E., Hughes, E. K., Puckett, M. K., Chen, R., Lowther, W. T., & Howlett, A. C. (2020). Cannabinoid receptor interacting protein 1a (CRIP1a) in health and disease. Biomolecules, 10(12), 1609.

Chen, D., Wu, H., Feng, X., Chen, Y., Lv, Z., Kota, V. G., ... & Wu, J. (2020). DNA Methylation of CNRIP1 Promotes Pathogenesis of Intrahepatic Cholangiocarcinoma Through Suppressing Parkin-Dependent PKM2 Ubiquitination. Hepatology (Baltimore, Md.).

Perez, S. M., Donegan, J. J., Boley, A. M., Aguilar, D. D., Giuffrida, A., & Lodge, D. J. (2019). Ventral hippocampal overexpression of Cannabinoid Receptor Interacting Protein 1 (CNRIP1) produces a schizophrenia-like phenotype in the rat. Schizophrenia research, 206, 263-270.

Booth, W. T., Walker, N. B., Lowther, W. T., & Howlett, A. C. (2019). Cannabinoid receptor interacting protein 1a (CRIP1a): function and structure. Molecules, 24(20), 3672.

Ahmed, M. H., & Zhang, Y. (2017). The Protein–Protein Interactions of Cannabinoid Receptor Interacting Protein 1a (CRIP1a) and Cannabinoid 1 Receptor: The Molecular Mechanism Study Through an Integrated Molecular Modeling Approach. In Handbook of Cannabis and Related Pathologies (pp. 680-690). Academic Press.

Singh, P., Ganjiwale, A., Howlett, A. C., & Cowsik, S. M. (2017). In silico interaction analysis of cannabinoid receptor interacting protein 1b (CRIP1b)–CB1 cannabinoid receptor. Journal of Molecular Graphics and Modelling, 77, 311-321.

Fin, L., Bergamin, G., Steiner, R. A., & Hughes, S. M. (2017). The Cannabinoid Receptor Interacting Proteins 1 of zebrafish are not required for morphological development, viability or fertility. Scientific reports, 7(1), 1-9.

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For research use only. Not intended for any clinical use.

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