Rabbit Anti-COL17A1 Recombinant Antibody (CAP522) (V2LY-1206-LY603)

Name: Rabbit Anti-COL17A1 Recombinant Antibody (CAP522)
SKU: V2LY-1206-LY603
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

CAP522

Application

IHC-P, WB

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1300-1400 of human Collagen XVII/COL17A1.

Host Species

Rabbit

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000
IHC-P	1:50-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.3, 50% glycerol, 0.05% BSA

Preservative

0.02% sodium azide

Concentration

2 mg/ml

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Collagen Type XVII Alpha 1 Chain

Entrez Gene ID

1308

UniProt ID

Q9UMD9

Function

May play a role in the integrity of hemidesmosome and the attachment of basal keratinocytes to the underlying basement membrane.

The 120 kDa linear IgA disease antigen is an anchoring filament component involved in dermal-epidermal cohesion. Is the target of linear IgA bullous dermatosis autoantibodies.

Biological Process

Cell-matrix adhesion Source: ProtInc
Collagen fibril organization Source: Reactome
Epidermis development Source: ProtInc
Extracellular matrix organization Source: GO_Central
Hemidesmosome assembly Source: UniProtKB
Regulation of immune response Source: Reactome

Cellular Location

Hemidesmosome; Membrane. Localized along the plasma membrane of the hemidesmosome.
120 kDa linear IgA disease antigen: Basement membrane. Exclusively localized to anchoring filaments. Localized to the epidermal side of split skin.
97 kDa linear IgA disease antigen: Basement membrane. Localized in the lamina lucida beneath the hemidesmosomes.

Involvement in disease

Generalized atrophic benign epidermolysis bullosa (GABEB):
A non-lethal, adult form of junctional epidermolysis bullosa characterized by life-long blistering of the skin, associated with hair and tooth abnormalities.
Epithelial recurrent erosion dystrophy (ERED):
A corneal dystrophy characterized by recurrent episodes of epithelial erosions from childhood, with occasional impairment of vision. Most patients have attacks of redness, photophobia, epiphora, and ocular pain. Exposure to sunlight or draught, dust and smoke and lack of sleep can precipitate attacks.

Topology

Cytoplasmic: 1-467
Helical: 468-488
Extracellular: 489-1497

PTM

The intracellular/endo domain is disulfide-linked.
Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
The ectodomain is shedded from the surface of keratinocytes resulting in a 120-kDa soluble form, also named as 120 kDa linear IgA disease antigen. The shedding is mediated by membrane-bound metalloproteases. This cleavage is inhibited by phosphorylation at Ser-544.

Yao, Y. Y., Zhang, Y., Xie, X. H., Chen, L., Zhu, F., & Zhou, M. (2020). Identification of a novel COL17A1 compound heterozygous mutation in a Chinese girl with non-herlitz junctional epidermolysis bullosa. Current Medical Science, 40(4), 795-800.

Mao, F., Li, D., Xin, Z., Du, Y., Wang, X., Xu, P., ... & Yao, J. (2020). High Expression of COL17A1 Predicts Poor Prognosis and Promotes the Tumor Progression via NF-κB Pathway in Pancreatic Adenocarcinoma. Journal of Oncology, 2020.

Yodsurang, V., Tanikawa, C., Miyamoto, T., Lo, P. H. Y., Hirata, M., & Matsuda, K. (2017). Identification of a novel p53 target, COL17A1, that inhibits breast cancer cell migration and invasion. Oncotarget, 8(34), 55790.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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