Rabbit Anti-COL4A5 Recombinant Antibody (CBYY-C0424) (V2LY-1206-LY678)

Name: Rabbit Anti-COL4A5 Recombinant Antibody (CBYY-C0424)
SKU: V2LY-1206-LY678
Rating: 5 (1 reviews)

See all COL4A5 antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

CBYY-C0424

Application

IHC-P

Immunogen

Recombinant fragment (around aa1400-1685) of human Collagen IV alpha5 protein.

Host Species

Rabbit

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
IHC-P	1-2 μg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.05% BSA

Preservative

0.05% sodium azide

Concentration

0.2 mg/ml

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Collagen Type IV Alpha 5 Chain

Entrez Gene ID

1287

UniProt ID

P29400

Function

Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.

Biological Process

Collagen-activated tyrosine kinase receptor signaling pathway Source: Ensembl
Collagen fibril organization Source: Reactome
Extracellular matrix organization Source: GO_Central
Neuromuscular junction development Source: Ensembl

Cellular Location

Basement membrane

Involvement in disease

Alport syndrome 1, X-linked (ATS1):
A syndrome that is characterized by progressive glomerulonephritis, renal failure, sensorineural deafness, specific eye abnormalities (lenticonous and macular flecks), and glomerular basement membrane defects. The disorder shows considerable heterogeneity in that families differ in the age of end-stage renal disease and the occurrence of deafness.

PTM

Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
Type IV collagens contain numerous cysteine residues which are involved in inter- and intramolecular disulfide bonding. 12 of these, located in the NC1 domain, are conserved in all known type IV collagens.
The trimeric structure of the NC1 domains is stabilized by covalent bonds between Lys and Met residues.

Barua, M., & Paterson, A. D. (2021). Population-based studies reveal an additive role of type IV collagen variants in hematuria and albuminuria. Pediatric Nephrology, 1-10.

Kuang, X., Sun, L., Wu, Y., & Huang, W. (2020). A novel missense mutation of COL4A5 gene alter collagen IV α5 chain to cause X-linked Alport syndrome in a Chinese family. Translational Pediatrics, 9(5), 587.

Yamamura, T., Horinouchi, T., Adachi, T., Terakawa, M., Takaoka, Y., Omachi, K., ... & Nozu, K. (2020). Development of an exon skipping therapy for X-linked Alport syndrome with truncating variants in COL4A5. Nature communications, 11(1), 1-8.

Kamura, M., Yamamura, T., Omachi, K., Suico, M. A., Nozu, K., Kaseda, S., ... & Kai, H. (2020). Trimerization and genotype–phenotype correlation of COL4A5 Mutants in Alport SYNDROME. Kidney international reports, 5(5), 718-726.

Said, S. M., Fidler, M. E., Valeri, A. M., McCann, B., Fiedler, W., Cornell, L. D., ... & Nasr, S. H. (2017). Negative staining for COL4A5 correlates with worse prognosis and more severe ultrastructural alterations in males with Alport syndrome. Kidney international reports, 2(1), 44-52.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Rabbit Anti-COL4A5 Recombinant Antibody (CBYY-C0424)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry