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Mouse Anti-COPB1 Recombinant Antibody (CBCNC-294) (V2LY-1206-LY754)


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Published Data
V2LY-1206-LY754-1
Mouse Anti-COPB1 Recombinant Antibody (CBCNC-294) (V2LY-1206-LY754) in IF
Immunofluorescence-based co-localization of SBP-FGFR1.GF with the ER/Golgi marker protein COPB (n = 3). Scale bar represents 20 µm. ...View More
Gregorczyk, P., Porębska, N., Żukowska, D., Chorążewska, A., Gędaj, A., Malinowska, A., ... & Opaliński, Ł. (2023). N-glycosylation acts as a switch for FGFR1 trafficking between the plasma membrane and nuclear envelope. Cell Communication and Signaling, 21(1), 177.
Distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
V2LY-1206-LY754-2
Mouse Anti-COPB1 Recombinant Antibody (CBCNC-294) (V2LY-1206-LY754) in IF
Representative immunocytochemical images of astrocytes stained with anti-β-COP and anti-TREK1 antibodies. Enlarged images on either side of the merged image indicate co-localization of β-COP and TREK1 in the plasma membrane and inside of astrocytes. Scale bar, 20 µm. ...View More
Kim, S. S., Bae, Y., Kwon, O., Kwon, S. H., Seo, J. B., Hwang, E. M., & Park, J. Y. (2022). β-COP Regulates TWIK1/TREK1 Heterodimeric Channel-Mediated Passive Conductance in Astrocytes. Cells, 11(20), 3322.
Distributed under Open Access license CC BY 4.0, without modification.
V2LY-1206-LY754-3
Mouse Anti-COPB1 Recombinant Antibody (CBCNC-294) (V2LY-1206-LY754) in WB and IP
Co-immunoprecipitation (Co-IP) assay in astrocytes. Whole-cell lysates were immunoprecipitated using anti-TREK1 and then blotted using anti-β-COP. ...View More
Kim, S. S., Bae, Y., Kwon, O., Kwon, S. H., Seo, J. B., Hwang, E. M., & Park, J. Y. (2022). β-COP Regulates TWIK1/TREK1 Heterodimeric Channel-Mediated Passive Conductance in Astrocytes. Cells, 11(20), 3322.
Distributed under Open Access license CC BY 4.0, without modification.
V2LY-1206-LY754-4
Mouse Anti-COPB1 Recombinant Antibody (CBCNC-294) (V2LY-1206-LY754) in IHC
Representative image of immunohistochemistry (IHC) in the hippocampus (CA1) and an enlarged image. β-COP (green) is expressed as abundantly as TREK1 (red) in GFAP-positive cells (white) of the hippocampus and colocalizes with TREK1 to a high degree (yellow). Scale bars, 50μm. ...View More
Kim, S. S., Bae, Y., Kwon, O., Kwon, S. H., Seo, J. B., Hwang, E. M., & Park, J. Y. (2022). β-COP Regulates TWIK1/TREK1 Heterodimeric Channel-Mediated Passive Conductance in Astrocytes. Cells, 11(20), 3322.
Distributed under Open Access license CC BY 4.0, without modification.
V2LY-1206-LY754-5
Mouse Anti-COPB1 Recombinant Antibody (CBCNC-294) (V2LY-1206-LY754) in WB
GORAB KO MCF-7 cells. KO cells were generated by CRISPR/Cas9 and their protein levels monitored by western blotting, in normal (DMEM) or mTORC1-inhibited conditions (Rapa 100 nM or starvation in HBSS for 3 h). Equal protein amounts were loaded. One representative western blot, out of n = 3 biological replicates, is shown. ...View More
Kaeser-Pebernard, S., Vionnet, C., Mari, M., Sankar, D. S., Hu, Z., Roubaty, C., ... & Dengjel, J. (2022). mTORC1 controls Golgi architecture and vesicle secretion by phosphorylation of SCYL1. Nature Communications, 13(1), 4685.
Distributed under Open Access license CC BY 4.0, without modification.
V2LY-1206-LY754-6
Mouse Anti-COPB1 Recombinant Antibody (CBCNC-294) (V2LY-1206-LY754) in IF
MARC-145 cells were infected with PRRSV SD95-21. At 10 hpi, cells were fixed, permeabilized and immunostained for nsp2TF. To label specific cellular compartments, the cells were co-stained with antibodies specific for ERGIC marker proteins ERGIC-53, COPA, COPB, or Golgin 97. Scale bars, 10 μm. ...View More
Guo, R., Yan, X., Li, Y., Cui, J., Misra, S., Firth, A. E., ... & Fang, Y. (2021). A swine arterivirus deubiquitinase stabilizes two major envelope proteins and promotes production of viral progeny. PLoS pathogens, 17(3), e1009403.
Distributed under Open Access license CC BY 4.0, without modification.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat, Horse, Dog, Cattle, Pig
Clone
CBCNC-294
Antibody Isotype
IgG2b, κ
Application
WB, IP, IF, IHC-P, ELISA

Basic Information

Immunogen
Amino acids 2-19 of rat COPB.
Host Species
Mouse
Specificity
Human, Mouse, Rat, Horse, Dog, Cattle, Pig
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000
IHC-P1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
0.1% gelatin
Preservative
0.09% sodium azide
Concentration
0.2 mg/ml
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Entrez Gene ID
Human1315
Mouse70349
Rat114023
UniProt ID
HumanP53618
MouseQ9JIF7
RatP23514
Function
The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors. Plays a functional role in facilitating the transport of kappa-type opioid receptor mRNAs into axons and enhances translation of these proteins. Required for limiting lipid storage in lipid droplets. Involved in lipid homeostasis by regulating the presence of perilipin family members PLIN2 and PLIN3 at the lipid droplet surface and promoting the association of adipocyte surface triglyceride lipase (PNPLA2) with the lipid droplet to mediate lipolysis (By similarity).

Involved in the Golgi disassembly and reassembly processes during cell cycle. Involved in autophagy by playing a role in early endosome function. Plays a role in organellar compartmentalization of secretory compartments including endoplasmic reticulum (ER)-Golgi intermediate compartment (ERGIC), Golgi, trans-Golgi network (TGN) and recycling endosomes, and in biosynthetic transport of CAV1. Promotes degradation of Nef cellular targets CD4 and MHC class I antigens by facilitating their trafficking to degradative compartments.
Biological Process
Endoplasmic reticulum to Golgi vesicle-mediated transport Source: GO_Central
Intracellular protein transport Source: InterPro
Intra-Golgi vesicle-mediated transport Source: UniProtKB
Neutrophil degranulation Source: Reactome
Retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum Source: Reactome
Viral process Source: UniProtKB-KW
Cellular Location
Golgi apparatus membrane; Cytoplasm; Cell membrane; COPI-coated vesicle membrane; Endoplasmic reticulum-Golgi intermediate compartment. The coatomer is cytoplasmic or polymerized on the cytoplasmic side of the Golgi, as well as on the vesicles/buds originating from it (By similarity). Proteolytic cleavage by CAPN8 triggers translocation from Golgi to cytoplasm (By similarity). Found in perinuclear vesicular-tubular clusters (VTCs) and in the Golgi region where associated with vesicles, buds and rims of the Golgi stack (By similarity). Occasionally present at the trans-side of Golgi, but mainly present at the cis-Golgi side in transitional areas (TA), on so-called peripheral elements (PE) consisting of tubules and vesicles located between the cup-shaped transitional elements (TE) of the rough endoplasmic reticulum (RER) and the cis-most Golgi cisternae (By similarity). Present in cytoplasm, not associated with visible coats or membranes, with a minor fraction present on small clusters of tubules and vesicles (By similarity). Some association with high-density and low-density microsomes and mitochondria/nuclei fraction (By similarity). Very little found in plasma membrane fraction (PubMed:20362547).
PTM
Proteolytically cleaved between Ser-528 and Ser-529 by CAPN8.
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For research use only. Not intended for any clinical use.

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