Mouse Anti-COX4I1 Recombinant Antibody (C4127) (V2LY-1206-LY863)

Name: Mouse Anti-COX4I1 Recombinant Antibody (C4127)
SKU: V2LY-1206-LY863
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

C4127

Application

WB, IF, IHC-P

Immunogen

Recombinant protein encompassing a sequence within the center region of human COX4.

Host Species

Mouse

Specificity

Human, Rat

Antibody Isotype

IgG2b

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:3,000
IF(ICC)	1:100-1:1,000
IHC-P	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 20% Glycerol

Preservative

None

Concentration

1 mg/ml

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Cytochrome C Oxidase Subunit 4I1

Entrez Gene ID

Human	1327
Rat	29445

UniProt ID

Human	P13073
Rat	P10888

Alternative Names

Cytochrome C Oxidase Subunit 4I1; Cytochrome C Oxidase Subunit IV Isoform 1; Cytochrome C Oxidase Polypeptide IV; Cytochrome C Oxidase Subunit IV; COX IV-1; COX4;

Function

Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunbit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.

Biological Process

Generation of precursor metabolites and energy Source: ProtInc
Mitochondrial electron transport, cytochrome c to oxygen Source: GO_Central
Response to nutrient Source: Ensembl

Cellular Location

Mitochondrion inner membrane

Involvement in disease

Mitochondrial complex IV deficiency, nuclear type 16 (MC4DN16):
An autosomal recessive mitochondrial disorder with onset in infancy and variable manifestations. MC4DN16 features include feeding difficulties, poor overall growth, short stature, microcephaly, developmental regression, severe hypotonia, and seizures. Cerebral and cerebellar atrophy, and abnormal lesions in the basal ganglia can be observed on brain imaging. Patient tissues show decreased levels and activity of mitochondrial respiratory complex IV.

Topology

Mitochondrial matrix: 23-98
Helical: 99-124
Mitochondrial intermembrane: 125-169

Li, L., Voloboueva, L., Griffiths, B. B., Xu, L., Giffard, R. G., & Stary, C. M. (2021). MicroRNA-338 inhibition protects against focal cerebral ischemia and preserves mitochondrial function in vitro in astrocytes and neurons via COX4I1. Mitochondrion, 59, 105-112.

Pajuelo Reguera, D., Čunátová, K., Vrbacký, M., Pecinová, A., Houštěk, J., Mráček, T., & Pecina, P. (2020). Cytochrome c oxidase subunit 4 isoform exchange results in modulation of oxygen affinity. Cells, 9(2), 443.

Abu-Libdeh, B., Douiev, L., Amro, S., Shahrour, M., Ta-Shma, A., Miller, C., ... & Saada, A. (2017). Mutation in the COX4I1 gene is associated with short stature, poor weight gain and increased chromosomal breaks, simulating Fanconi anemia. European Journal of Human Genetics, 25(10), 1142-1146.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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