Mouse Anti-CPSF2 Recombinant Antibody (CBXC-1743) (V2LY-1206-LY986)

Name: Mouse Anti-CPSF2 Recombinant Antibody (CBXC-1743)
SKU: V2LY-1206-LY986
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-CPSF2 Recombinant Antibody (CBXC-1743) (V2LY-1206-LY986) in WB

Whole cell lysates from THP-1 cells treated with PMA for 0, 1, 6, 18 and 24 hours were separated by 10% SDS-PAGE and western blotting was performed for the indicated subunits of the C/P complex, with β-actin as the loading control. Each western blot (left panel) was performed in three biological replicates of the differentiation process and the quantified data is shown in the right panel. ...View More

Mukherjee, S., Graber, J. H., & Moore, C. L. (2023). Macrophage differentiation is marked by increased abundance of the mRNA 3'end processing machinery, altered poly (A) site usage, and sensitivity to the level of CstF64. Frontiers in immunology, 14, 1091403.

Distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Mouse Anti-CPSF2 Recombinant Antibody (CBXC-1743) (V2LY-1206-LY986) in WB

ICP4 containing complexes include TFIIH, cleavage and polyadenylation complex and chromatin remodeling complexes.
Western blot analysis of nuclear extracts (NE) or affinity purified (PD) samples collected at 6 hpi using either wtICP4 (KOS) or TAP-wtICP4 (TAP) expressing viruses. Antibodies directed against (A) p80 and p62 of TFIIH, (B) CPSF2 and CPSF7 of the cleavage and polyadenylation complex and (C) Brahma of Swi/Snf, CHD3 of the Nurd complex, and RuvBL1 and RuvBL2 of the Ino80 complex were used. ...View More

Wagner, L. M., & DeLuca, N. A. (2013). Temporal association of herpes simplex virus ICP4 with cellular complexes functioning at multiple steps in PolII transcription. PloS one, 8(10), e78242.

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBXC-1743

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 258-295 within an internal region of human CPSF2.

Host Species

Mouse

Specificity

Human, Mouse, Rat, Dog, Cattle, Horse

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

0.1% gelatin

Preservative

0.09% sodium azide

Concentration

0.2 mg/ml

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Cleavage And Polyadenylation Specific Factor 2

Entrez Gene ID

Human	53981
Mouse	51786
Rat	299256

UniProt ID

Human	Q9P2I0
Mouse	O35218
Rat	A6JEJ7

Function

Component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. Involved in the histone 3' end pre-mRNA processing.

Biological Process

mRNA 3'-end processing Source: Reactome
mRNA 3'-end processing by stem-loop binding and cleavage Source: UniProtKB
mRNA export from nucleus Source: Reactome
mRNA polyadenylation Source: GO_Central
mRNA splicing, via spliceosome Source: Reactome
Pre-mRNA cleavage required for polyadenylation Source: GO_Central
Termination of RNA polymerase II transcription Source: Reactome

Cellular Location

Nucleus

Hiwasa, T., Wang, H., Goto, K. I., Mine, S., Machida, T., Kobayashi, E., ... & Iwadate, Y. (2021). Serum anti-DIDO1, anti-CPSF2, and anti-FOXJ2 antibodies as predictive risk markers for acute ischemic stroke. BMC medicine, 19(1), 1-25.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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