Mouse Anti-CPSF6 Recombinant Antibody (CBFYC-2168) (V2LY-1206-LY995)

Name: Mouse Anti-CPSF6 Recombinant Antibody (CBFYC-2168)
SKU: V2LY-1206-LY995
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Published Data

Mouse Anti-CPSF6 Recombinant Antibody (CBFYC-2168) (V2LY-1206-LY995) in WB

Western blot analysis of the expression of selected proteins in cytosolic (C) and nuclear (N) extracts of SW480-ADH Mock and Snail1 cells. HNRNPH3 isoforms (1-4) are indicated. The numbers below the gels indicate the quantification of the change in expression between Snail1 and Mock cells after normalization to β-Tubulin or Lamin B (cytosolic and nuclear control, respectively). The statistical significance of the nuclear expression changes promoted by Snail1 was assessed by two-tailed unpaired Student's t-test. It was p<0.001 for 14-3-3ε, 14-3-3σ, 14-3-3ζ, CPSF6 and the isoforms 3 and 4 of HNRNPH3; p<0.01 for 14-3-3τ; and p<0.05 for 14-3-3β and PA2G4. The regulation of SFPQ (p = 0.057) and that of the isoforms 1+2 of HNRNPH3 (p = 0.058) did not reached the statistical significance, but a clear tendency was observed. ...View More

Larriba, M. J., Casado-Vela, J., Pendas-Franco, N., Peña, R., García de Herreros, A., Berciano, M. T., ... & Muñoz, A. (2010). Novel snail1 target proteins in human colon cancer identified by proteomic analysis. PloS one, 5(4), e10221.

Distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYC-2168

Application

ELISA, IF, WB

Immunogen

CPSF6 (aa37-136) partial recombinant protein.

Host Species

Mouse

Specificity

Human, Mouse

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
IF(ICC)	10 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

None

Concentration

Batch dependent

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

cleavage and polyadenylation specific factor 6, 68kDa

Entrez Gene ID

Human	11052
Mouse	432508

UniProt ID

Human	Q16630
Mouse	Q6NVF9

Function

Component of the cleavage factor Im (CFIm) complex that functions as an activator of the pre-mRNA 3'-end cleavage and polyadenylation processing required for the maturation of pre-mRNA into functional mRNAs (PubMed:9659921, PubMed:8626397, PubMed:14690600, PubMed:29276085).

CFIm contributes to the recruitment of multiprotein complexes on specific sequences on the pre-mRNA 3'-end, so called cleavage and polyadenylation signals (pA signals) (PubMed:9659921, PubMed:8626397, PubMed:14690600).

Most pre-mRNAs contain multiple pA signals, resulting in alternative cleavage and polyadenylation (APA) producing mRNAs with variable 3'-end formation (PubMed:23187700, PubMed:29276085).

The CFIm complex acts as a key regulator of cleavage and polyadenylation site choice during APA through its binding to 5'-UGUA-3' elements localized in the 3'-untranslated region (UTR) for a huge number of pre-mRNAs (PubMed:20695905, PubMed:29276085).

CPSF6 enhances NUDT21/CPSF5 binding to 5'-UGUA-3' elements localized upstream of pA signals and promotes RNA looping, and hence activates directly the mRNA 3'-processing machinery (PubMed:15169763, PubMed:29276085, PubMed:21295486).

Plays a role in mRNA export (PubMed:19864460).

(Microbial infection) Binds HIV-1 capsid-nucleocapsid (HIV-1 CA-NC) complexes and might thereby promote the integration of the virus in the nucleus of dividing cells (in vitro).

Biological Process

Messenger ribonucleoprotein complex assembly Source: UniProtKB
mRNA alternative polyadenylation Source: UniProtKB
mRNA processing Source: UniProtKB
Positive regulation of RNA export from nucleus Source: UniProtKB
Pre-mRNA cleavage required for polyadenylation Source: UniProtKB
Protein heterotetramerization Source: UniProtKB
Protein tetramerization Source: UniProtKB

Cellular Location

Nucleus; Nucleoplasm; Nucleus speckle; Cytoplasm. Shuttles between the nucleus and the cytoplasm in a transcription- and XPO1/CRM1-independent manner, most probably in complex with the cleavage factor Im complex (CFIm) (PubMed:19864460). Colocalizes with PSPC1 in punctate subnuclear structures often located adjacent to nuclear speckles, called paraspeckles, and corresponding to interchromatin granules-associated zones (IGAZs) (PubMed:17267687). Distribution in speckles and paraspeckles varies during the cell cycle (PubMed:17267687). Associates at sites of active transcription on nascent perichromatin fibrils (PFs) and perichromatin granules (PubMed:17267687). Nuclear import is mediated via interaction with TNPO3 independently of CPSF6 phosphorylation status (PubMed:30916345).

PTM

Phosphorylated (PubMed:29276085). Phosphorylated in the Arg/Ser-rich domain by SRPK1, in vitro (PubMed:29276085).
Symmetrically dimethylated on arginine residues in the GAR motif by PRMT5 in a WDR77- and CLNS1A-dependent manner (PubMed:20562214). Asymmetrically dimethylated on arginine residues in the GAR motif by PRMT1 (PubMed:20562214).

Zheng, Y., Schubert, H. L., Singh, P. K., Martins, L. J., Engelman, A. N., D’Orso, I., ... & Planelles, V. (2021). Cleavage and Polyadenylation Specificity Factor 6 Is Required for Efficient HIV-1 Latency Reversal. Mbio, 12(3), e01098-21.

Tan, S., Zhang, M., Shi, X., Ding, K., Zhao, Q., Guo, Q., ... & Zhao, X. (2021). CPSF6 links alternative polyadenylation to metabolism adaption in hepatocellular carcinoma progression. Journal of Experimental & Clinical Cancer Research, 40(1), 1-16.

Han, X., Jin, C., Zheng, G., Li, Y., Wang, Y., Zhang, E., ... & Cai, Z. (2021). Acute myeloid leukemia with CPSF6–RARG fusion resembling acute promyelocytic leukemia with extramedullary infiltration. Therapeutic advances in hematology, 12, 2040620720976984.

Zhong, Z., Ning, J., Boggs, E. A., Jang, S., Wallace, C., Telmer, C., ... & Ambrose, Z. (2021). Cytoplasmic CPSF6 regulates HIV-1 capsid trafficking and infection in a cyclophilin A-dependent manner. MBio, 12(2), e03142-20.

Wang, X., Xu, P., Cheng, F., Li, Y., Wang, Z., Hao, S., ... & Qiu, J. (2020). Cellular cleavage and polyadenylation specificity factor 6 (CPSF6) mediates nuclear import of human bocavirus 1 NP1 protein and modulates viral capsid protein expression. Journal of Virology, 94(2), e01444-19.

Achuthan, V., Perreira, J. M., Ahn, J. J., Brass, A. L., & Engelman, A. N. (2019). Capsid-CPSF6 interaction: Master regulator of nuclear HIV-1 positioning and integration. Journal of life sciences (Westlake Village, Calif.), 1(1), 39.

Jang, S., Cook, N. J., Pye, V. E., Bedwell, G. J., Dudek, A. M., Singh, P. K., ... & Engelman, A. N. (2019). Differential role for phosphorylation in alternative polyadenylation function versus nuclear import of SR-like protein CPSF6. Nucleic acids research, 47(9), 4663-4683.

Bejarano, D. A., Peng, K., Laketa, V., Börner, K., Jost, K. L., Lucic, B., ... & Kräusslich, H. G. (2019). HIV-1 nuclear import in macrophages is regulated by CPSF6-capsid interactions at the nuclear pore complex. Elife, 8, e41800.

Qin, Y. Z., Huang, X. J., & Zhu, H. H. (2018). Identification of a novel CPSF6-RARG fusion transcript in acute myeloid leukemia resembling acute promyelocytic leukemia. Leukemia, 32(10), 2285-2287.

Sasado, T., Kondoh, H., Furutani-Seiki, M., & Naruse, K. (2017). Mutation in cpsf6/CFIm68 (cleavage and polyadenylation specificity factor subunit 6) causes short 3'UTRs and disturbs gene expression in developing embryos, as revealed by an analysis of primordial germ cell migration using the medaka mutant naruto. PloS one, 12(3), e0172467.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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