Mouse Anti-CPV2 Recombinant Antibody (CPV1-2A1) (CBMAB-V208-1333-CQ)

Name: Mouse Anti-CPV2 Recombinant Antibody (CPV1-2A1)
SKU: CBMAB-V208-1333-CQ
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CPV1-2A1

Application

ELISA, IHC-P

Immunogen

Canine Parvovirus.

Host Species

Mouse

Specificity

Canine Parvovirus

Antibody Isotype

IgG2a

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

0.09% sodium azide

Concentration

1 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Canine parvovirus type 2

Introduction

Canine parvovirus type 2 (CPV2, colloquially parvo) is a contagious virus mainly affecting dogs, and thought to originate in cats. The current consensus is that the feline panleukopenia virus mutated into CPV2. Parvo is highly contagious and is spread from dog to dog by direct or indirect contact with their feces. Vaccines can prevent this infection, but mortality can reach 91% in untreated cases. Treatment often involves veterinary hospitalization. Canine parvovirus may infect other mammals including foxes, wolves, cats, and skunks; however, it will not infect humans.

Alternative Names

Canine parvovirus type 2; CPV2; parvo

Function

Capsid protein self-assembles to form an icosahedral capsid with a T=1 symmetry, about 22 nm in diameter, and consisting of 60 copies of two size variants of the capsid proteins, VP1 and VP2, which differ by the presence of an N-terminal extension in the minor protein VP1. The capsid encapsulates the genomic ssDNA. Capsid proteins are responsible for the attachment to host cell receptor TFRC. This attachment induces virion internalization predominantly through clathrin-endocytosis. Binding to the host receptors also induces capsid rearrangements leading to surface exposure of VP1 N-terminus, specifically its phospholipase A2-like region and nuclear localization signal(s). VP1 N-terminus might serve as a lipolytic enzyme to breach the endosomal membrane during entry into host cell. Intracytoplasmic transport involves microtubules and interaction between capsid proteins and host dynein. Exposure of nuclear localization signal probably allows nuclear import of capsids (By similarity).

Biological Process

Adhesion receptor-mediated virion attachment to host cell Source: UniProtKB-KW
Clathrin-dependent endocytosis of virus by host cell Source: UniProtKB-KW
Entry receptor-mediated virion attachment to host cell Source: UniProtKB-KW
Microtubule-dependent intracellular transport of viral material towards nucleus Source: UniProtKB-KW
Permeabilization of host organelle membrane involved in viral entry into host cell Source: UniProtKB-KW
Viral entry via permeabilization of inner membrane Source: UniProtKB-KW
Viral penetration into host nucleus Source: UniProtKB-KW

Castillo, C., Neira, V., Aniñir, P., Grecco, S., Pérez, R., Panzera, Y., ... & Ortega, R. (2020). First molecular identification of canine parvovirus type 2 (CPV2) in Chile reveals high occurrence of CPV2c antigenic variant. Frontiers in veterinary science, 194.

Zhao, W., Wang, X., Li, Y., & Li, Y. (2020). Administration with vaccinia virus encoding canine parvovirus 2 vp2 elicits systemic immune responses in mice and dogs. Viral Immunology, 33(6), 434-443.

Clark, N. J., Seddon, J. M., Kyaw-Tanner, M., Al-Alawneh, J., Harper, G., McDonagh, P., & Meers, J. (2018). Emergence of canine parvovirus subtype 2b (CPV-2b) infections in Australian dogs. Infection, Genetics and Evolution, 58, 50-55.

Duque-García, Y., Echeverri-Zuluaga, M., Trejos-Suarez, J., & Ruiz-Saenz, J. (2017). Prevalence and molecular epidemiology of Canine parvovirus 2 in diarrheic dogs in Colombia, South America: A possible new CPV-2a is emerging?. Veterinary microbiology, 201, 56-61.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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