Mouse Anti-CREB3L3 Recombinant Antibody (CBWJC-3070) (CBMAB-C4237WJ)

Name: Mouse Anti-CREB3L3 Recombinant Antibody (CBWJC-3070)
SKU: CBMAB-C4237WJ
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-CREB3L3 Recombinant Antibody (CBWJC-3070) (CBMAB-C4237WJ) in IF

Immunofluorescence staining for CREB3L3, a liver-specific marker. ...View More

Huaman, J., Naidoo, M., Zang, X., & Ogunwobi, O. O. (2019). Fibronectin regulation of integrin B1 and SLUG in circulating tumor cells. Cells, 8(6), 618.

Distributed under Open Access license CC BY 4.0, without modification.

Mouse Anti-CREB3L3 Recombinant Antibody (CBWJC-3070) (CBMAB-C4237WJ) in WB

Western blotting for CREB3L3. ...View More

Huaman, J., Naidoo, M., Zang, X., & Ogunwobi, O. O. (2019). Fibronectin regulation of integrin B1 and SLUG in circulating tumor cells. Cells, 8(6), 618.

Distributed under Open Access license CC BY 4.0, without modification.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBWJC-3070

Application

WB, IP, IF, IHC-P, ELISA

Immunogen

Amino acids 1-223 mapping at the N-terminus of mouse CREB3L3.

Host Species

Mouse

Specificity

Mouse

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

0.1% gelatin

Preservative

0.09% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

CREB3L3

Introduction

CREB3L3 (CAMP Responsive Element Binding Protein 3 Like 3) is a Protein Coding gene. Diseases associated with CREB3L3 include Hyperlipoproteinemia, Type V and Hepatocellular Carcinoma. Among its related pathways are Aldosterone synthesis and secretion and Circadian entrainment. Gene Ontology (GO) annotations related to this gene include DNA binding transcription factor activity and sequence-specific DNA binding. An important paralog of this gene is CREB3L4.

Entrez Gene ID

208677

UniProt ID

Q91XE9

Alternative Names

CAMP Responsive Element Binding Protein 3 Like 3; CREBH; Cyclic AMP-Responsive Element-Binding Protein 3-Like Protein 3; CAMP-Responsive Element-Binding Protein 3-Like Protein 3; CREB/ATF Family Transcription Factor; Transcription Factor CREB-H; HYST1481; CREB-H;

Function

Transcription factor that may act during endoplasmic reticulum stress by activating unfolded protein response target genes. Activated in response to cAMP stimulation. In vitro, binds to the cAMP response element (CRE) and box-B element. Activates transcription through box-B element. Activates transcription through CRE (By similarity).

May function synergistically with ATF6. In acute inflammatory response, may activate expression of acute phase response (APR) genes. May be involved in growth suppression. Regulates FGF21 transcription (By similarity).

Biological Process

Endoplasmic reticulum unfolded protein response Source: Reactome
Positive regulation of acute inflammatory response Source: ParkinsonsUK-UCL
Positive regulation of transcription by RNA polymerase II Source: NTNU_SB
Positive regulation of transcription from RNA polymerase II promoter in response to endoplasmic reticulum stress Source: ParkinsonsUK-UCL
Regulation of transcription by RNA polymerase II Source: GO_Central

Cellular Location

Endoplasmic reticulum membrane
Processed cyclic AMP-responsive element-binding protein 3-like protein 3: Nucleus. Under ER stress the cleaved N-terminal cytoplasmic domain translocates into the nucleus.

Topology

Cytoplasmic: 1-322
Helical: 323-343
Lumenal: 344-461

PTM

Controlled by regulated intramembrane proteolysis (RIP). Following ER stress a fragment containing the cytoplasmic transcription factor domain is released by proteolysis. The cleavage seems to be performed sequentially by site-1 and site-2 proteases (PS1 and PS2).
N- and O-glycosylated. N-glycosylation is required for optimal proteolytic activation. O-glycosylated with core 1 or possibly core 8 glycans.
Ubiquitinated at Lys-294 by SYNV1/HRD1 via 'Lys-27'-linked ubiquitin.

Nakagawa, Y., Wang, Y., Han, S. I., Okuda, K., Oishi, A., Yagishita, Y., ... & Shimano, H. (2021). Enterohepatic transcription factor CREB3L3 protects atherosclerosis via SREBP competitive inhibition. Cellular and Molecular Gastroenterology and Hepatology, 11(4), 949-971.

Pradhan-Sundd, T. (2021). Making the Best of a Competition: the CREB3L3–SREBP Axis in Arteriosclerosis. Cellular and Molecular Gastroenterology and Hepatology, 11(4), 1199-1201.

McCann, M. A., Li, Y., Muñoz, M., Gil, V., Qiang, G., Cordoba-Chacon, J., ... & Liew, C. W. (2021). Adipose expression of CREB3L3 modulates body weight during obesity. Scientific reports, 11(1), 1-16.

Ruppert, P. M., Park, J. G., Xu, X., Hur, K. Y., Lee, A. H., & Kersten, S. (2019). Transcriptional profiling of PPARα−/− and CREB3L3−/− livers reveals disparate regulation of hepatoproliferative and metabolic functions of PPARα. BMC genomics, 20(1), 1-19.

Dewaele, B., Libbrecht, L., Levy, G., Brichard, B., Vanspauwen, V., Sciot, R., & Debiec‐Rychter, M. (2017). A novel EWS‐CREB3L3 gene fusion in a mesenteric sclerosing epithelioid fibrosarcoma. Genes, Chromosomes and Cancer, 56(9), 695-699.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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