Mouse Anti-CRISP2 Recombinant Antibody (CBYY-C2860) (V2LY-1206-LY1168)

Name: Mouse Anti-CRISP2 Recombinant Antibody (CBYY-C2860)
SKU: V2LY-1206-LY1168
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-CRISP2 Recombinant Antibody (CBYY-C2860) (V2LY-1206-LY1168) in WB

Western blot analysis of CATSPER1, PATE1, CRISP2 and Tubulin proteins in A-spermatozoa and B-spermatozoa collected from asthenozoospermic patients before (pre-A; pre-B) and after (post-A; post-B) an oral amino acid supplementation therapy. ...View More

Manfrevola, F., Ferraro, B., Sellitto, C., Rocco, D., Fasano, S., Pierantoni, R., & Chianese, R. (2021). CRISP2, CATSPER1 and PATE1 expression in human asthenozoospermic semen. Cells, 10(8), 1956.

Distributed under Open Access license CC BY 4.0, without modification.

Mouse Anti-CRISP2 Recombinant Antibody (CBYY-C2860) (V2LY-1206-LY1168) in IHC

Representative immunohistochemical (IHC) staining (A) and semiquantitative analysis (B) of Ki67, KRT17 and CRISP2 during carcinogenesis. Bar scale: 50 μm. IHC results are presented with IOD value/area in different groups. The Kruskal-Wallis rank test was performed on the IHC results and p < 0.05 was regarded as statistically significant ...View More

Li, Z., Chen, J., Zhao, S., Li, Y., Zhou, J., Liang, J., & Tang, H. (2021). Discovery and validation of novel biomarkers for detection of cervical cancer. Cancer medicine, 10(6), 2063-2074.

Distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Datasheet

Summary

Host Animal

Mouse

Specificity

Human, Mouse, Rat

Clone

CBYY-C2860

Antibody Isotype

IgG1, κ

Application

WB, IP, IF, ELISA

Basic Information

Immunogen

Amino acids 171-243 mapping at the C-terminus of human CRISP-2.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

0.1% gelatin

Preservative

0.09% sodium azide

Concentration

0.2 mg/ml

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

Cysteine Rich Secretory Protein 2

Entrez Gene ID

Human	7180
Mouse	22024
Rat	360445

UniProt ID

Human	P16562
Mouse	P16563
Rat	O88205

Function

May regulate some ion channels' activity and therebye regulate calcium fluxes during sperm capacitation.

Biological Process

Cell-cell adhesion Source: Ensembl

Cellular Location

Secreted

More Infomation

References

Gao, F., Wang, P., Wang, K., Fan, Y., Chen, Y., Chen, Y., ... & Wei, H. (2021). Investigation Into the Relationship Between Sperm Cysteine-Rich Secretory Protein 2 (CRISP2) and Sperm Fertilizing Ability and Fertility of Boars. Frontiers in veterinary science, 8, 435.

Arévalo, L., Brukman, N. G., Cuasnicú, P. S., & Roldan, E. R. (2020). Evolutionary analysis of genes coding for Cysteine-RIch Secretory Proteins (CRISPs) in mammals. BMC evolutionary biology, 20(1), 1-13.

Gholami, D., Yazdi, R. S., Jami, M. S., Ghasemi, S., Gilani, M. A. S., Sadeghinia, S., & Teimori, H. (2020). The expression of Cysteine-Rich Secretory Protein 2 (CRISP2) and miR-582-5p in seminal plasma fluid and spermatozoa of infertile men. Gene, 730, 144261.

Zhou, J. H., Zhou, Q. Z., Yang, J. K., Lyu, X. M., Bian, J., Guo, W. B., ... & Liu, C. D. (2017). MicroRNA-27a-mediated repression of cysteine-rich secretory protein 2 translation in asthenoteratozoospermic patients. Asian Journal of Andrology, 19(5), 591.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Documents

Datasheet
SDS
Request for COA

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Second antibody and isotype control

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