Mouse Anti-CRTC1 Recombinant Antibody (CBT1215) (V2LY-0625-LY2935)

Name: Mouse Anti-CRTC1 Recombinant Antibody (CBT1215)
SKU: V2LY-0625-LY2935
Rating: 5 (1 reviews)

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Tested Data

Mouse Anti-CRTC1 Recombinant Antibody (CBT1215) (V2LY-0625-LY2935) in FC

Mouse Anti-CRTC1 Recombinant Antibody (CBT1215) (V2LY-0625-LY2935) in WB

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBT1215

Application

WB, FC, IF, ICC

Immunogen

Purified recombinant human TORC1 (N-terminus) protein fragments expressed in E.coli.

Host Species

Mouse

Specificity

Human, Mouse, Rat, Pig

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
FC	1:100-1:200
IF	1:100-1:500
WB	1:1,000-1:2,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Tris-Glycine, Glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

CREB Regulated Transcription Coactivator 1

Entrez Gene ID

23373

UniProt ID

Q6UUV9

Function

Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates the expression of specific CREB-activated genes such as the steroidogenic gene, StAR. Potent coactivator of PGC1alpha and inducer of mitochondrial biogenesis in muscle cells. In the hippocampus, involved in late-phase long-term potentiation (L-LTP) maintenance at the Schaffer collateral-CA1 synapses. May be required for dendritic growth of developing cortical neurons (By similarity).

In concert with SIK1, regulates the light-induced entrainment of the circadian clock. In response to light stimulus, coactivates the CREB-mediated transcription of PER1 which plays an important role in the photic entrainment of the circadian clock.

(Microbial infection) Plays a role of coactivator for TAX activation of the human T-cell leukemia virus type 1 (HTLV-1) long terminal repeats (LTR).

Biological Process

Energy homeostasis Source: Ensembl
Entrainment of circadian clock by photoperiod Source: UniProtKB
Memory Source: Ensembl
Negative regulation of membrane hyperpolarization Source: Ensembl
Positive regulation of CREB transcription factor activity Source: UniProtKB
Positive regulation of dendrite development Source: Ensembl
Positive regulation of long-term synaptic potentiation Source: Ensembl
Positive regulation of transcription by RNA polymerase II Source: UniProtKB
Postsynapse to nucleus signaling pathway Source: Ensembl
Protein homotetramerization Source: InterPro
Rhythmic process Source: UniProtKB-KW
Viral process Source: UniProtKB-KW

Cellular Location

Cytoplasm; Nucleus. Cytoplasmic when phosphorylated by SIK or AMPK and when sequestered by 14-3-3 proteins (PubMed:16817901). Translocated to the nucleus on Ser-151 dephosphorylation, instigated by a number of factors including calcium ion and cAMP levels (PubMed:15589160). Light stimulation triggers a nuclear accumulation in the suprachiasmatic nucleus (SCN) of the brain (By similarity).

Involvement in disease

A chromosomal aberration involving CRTC1 is found in mucoepidermoid carcinomas, benign Warthin tumors and clear cell hidradenomas. Translocation t(11;19)(q21;p13) with MAML2. The fusion protein consists of the N-terminus of CRTC1 joined to the C-terminus of MAML2. The reciprocal fusion protein consisting of the N-terminus of MAML2 joined to the C-terminus of CRTC1 has been detected in a small number of mucoepidermoid carcinomas.

PTM

Phosphorylation/dephosphorylation states of Ser-151 are required for regulating transduction of CREB activity. TORCs are inactive when phosphorylated, and active when dephosphorylated at this site. This primary site of phosphorylation is mediated by SIKs (SIK1 and SIK2), is regulated by cAMP and calcium levels and is dependent on the phosphorylation of SIKs by LKB1 (By similarity).

Rossetti, C., Cherix, A., Guiraud, L. F., & Cardinaux, J. R. (2022). New Insights Into the Pivotal Role of CREB-Regulated Transcription Coactivator 1 in Depression and Comorbid Obesity. Frontiers in Molecular Neuroscience, 15.

Pan, Y., He, X., Li, C., Li, Y., Li, W., Zhang, H., ... & Ma, H. (2021). Neuronal activity recruits the CRTC1/CREB axis to drive transcription-dependent autophagy for maintaining late-phase LTD. Cell Reports, 36(3), 109398.

Jiang, B., Wang, H., Wang, J. L., Wang, Y. J., Zhu, Q., Wang, C. N., ... & Li, J. X. (2019). Hippocampal Salt-Inducible Kinase 2 Plays a Role in Depression via the CREB-Regulated Transcription Coactivator 1–cAMP Response Element Binding–Brain-Derived Neurotrophic Factor Pathway. Biological psychiatry, 85(8), 650-666.

Ni, S., Huang, H., He, D., Chen, H., Wang, C., Zhao, X., ... & Zhang, J. (2019). Adeno‐associated virus‐mediated over‐expression of CREB‐regulated transcription coactivator 1 in the hippocampal dentate gyrus ameliorates lipopolysaccharide‐induced depression‐like behaviour in mice. Journal of Neurochemistry, 149(1), 111-125.

Morhenn, K., Quentin, T., Wichmann, H., Steinmetz, M., Prondzynski, M., Söhren, K. D., ... & Oetjen, E. (2019). Mechanistic role of the CREB-regulated transcription coactivator 1 in cardiac hypertrophy. Journal of molecular and cellular cardiology, 127, 31-43.

Kim, M. K., Kwon, J. O., Song, M. K., Kim, B., Kim, H., Lee, Z. H., ... & Kim, H. H. (2019). Salt-inducible kinase 1 regulates bone anabolism via the CRTC1–CREB–Id1 axis. Cell death & disease, 10(11), 1-15.

Saura, C. A., & Cardinaux, J. R. (2017). Emerging roles of CREB-regulated transcription coactivators in brain physiology and pathology. Trends in neurosciences, 40(12), 720-733.

Parra-Damas, A., Chen, M., Enriquez-Barreto, L., Ortega, L., Acosta, S., Perna, J. C., ... & Saura, C. A. (2017). CRTC1 function during memory encoding is disrupted in neurodegeneration. Biological psychiatry, 81(2), 111-123.

Parra-Damas, A., Rubió-Ferrarons, L., Shen, J., & Saura, C. A. (2017). CRTC1 mediates preferential transcription at neuronal activity-regulated CRE/TATA promoters. Scientific reports, 7(1), 1-13.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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For research use only. Not intended for any clinical use.

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