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Mouse Anti-CSDC2 Recombinant Antibody (C5303) (V2LY-1206-LY1360)

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Published Data
V2LY-1206-LY1360-1
Mouse Anti-CSE1L Recombinant Antibody (3D8) (V2LY-1206-LY1360) in WB
Presence of hyper-, hypo-, and non-phosphorylated CSE1L in tumor cells as analyzed by antibodies against CSE1L and phosphorylated CSE1L. ...View More
Lee, W. R., Shen, S. C., Shih, Y. H., Chou, C. L., Tseng, J. T. P., Chin, S. Y., ... & Jiang, M. C. (2015). Early decline in serum phospho-CSE1L levels in vemurafenib/sunitinib-treated melanoma and sorafenib/lapatinib-treated colorectal tumor xenografts. Journal of Translational Medicine, 13, 1-12.
Distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
V2LY-1206-LY1360-2
Mouse Anti-CSE1L Recombinant Antibody (3D8) (V2LY-1206-LY1360) in Immunoblot
Vemurafenib treatment inhibited the phosphorylation of ERK1/2 and CSE1L. The levels of hyper-phosphorylated CSE1L, hypo-phosphorylated CSE1L, and phospho-ERK1/2 in A375 melanoma cells treated with or without 1 μM vemurafenib for 24 h were subjected to immunoblotting with anti-CSE1L (clone 3D8), anti-phospho-CSE1L, and anti-phospho-ERK1/2 antibodies. β-actin levels were assayed as a control. ...View More
Lee, W. R., Shen, S. C., Shih, Y. H., Chou, C. L., Tseng, J. T. P., Chin, S. Y., ... & Jiang, M. C. (2015). Early decline in serum phospho-CSE1L levels in vemurafenib/sunitinib-treated melanoma and sorafenib/lapatinib-treated colorectal tumor xenografts. Journal of Translational Medicine, 13, 1-12.
Distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Datasheet
SDS
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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal
Mouse
Clone
C5303
Application
WB, IP, IF, ELISA
Immunogen
Amino acids 11-50 mapping near the N-terminus of human PIPPIN.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, κ
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
0.1% gelatin
Preservative
0.09% sodium azide
Concentration
0.2 mg/ml
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.
More Infomation

Target

Full Name
Cold Shock Domain Containing C2
Entrez Gene ID
Human27254
Mouse105859
Rat266600
UniProt ID
HumanQ9Y534
MouseQ91YQ3
RatQ63430
Function
RNA-binding factor which binds specifically to the very 3'-UTR ends of both histone H1 and H3.3 mRNAs, encompassing the polyadenylation signal. Might play a central role in the negative regulation of histone variant synthesis in the developing brain (By similarity).
Biological Process
mRNA processing Source: UniProtKB-KW
Regulation of mRNA stability Source: GO_Central
Cellular Location
Nucleus; Cytoplasm. PIPPin-RNA complexes are located to the nucleus.

Vallejo, G., Mestre‐Citrinovitz, A. C., Winterhager, E., & Saragüeta, P. E. (2019). CSDC2, a cold shock domain RNA‐binding protein in decidualization. Journal of Cellular Physiology, 234(1), 740-748.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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