Mouse Anti-CSPG5 Recombinant Antibody (CBWJN-1078) (CBMAB-N0695-WJ)

Name: Mouse Anti-CSPG5 Recombinant Antibody (CBWJN-1078)
SKU: CBMAB-N0695-WJ
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBWJN-1078

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 181-276 mapping within an internal region of human Neuroglycan C.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:100-1:1,000
IP	1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

More Infomation

Target

Full Name

Chondroitin Sulfate Proteoglycan 5

Entrez Gene ID

10675

UniProt ID

O95196

Function

May function as a growth and differentiation factor involved in neuritogenesis. May induce ERBB3 activation.

Biological Process

Cell projection morphogenesis Source: GO_Central
Chondroitin sulfate biosynthetic process Source: Reactome
Chondroitin sulfate catabolic process Source: Reactome
Cytoskeleton organization Source: UniProtKB
Dermatan sulfate biosynthetic process Source: Reactome
Glial cell projection elongation Source: UniProtKB
Intracellular transport Source: UniProtKB
Nervous system development Source: UniProtKB
Positive regulation of substrate adhesion-dependent cell spreading Source: UniProtKB
Regulation of growth Source: UniProtKB-KW
Regulation of synaptic vesicle exocytosis Source: Ensembl
Trans-synaptic signaling, modulating synaptic transmission Source: GO_Central

Cellular Location

Endoplasmic reticulum membrane; Cell membrane; Synaptic cell membrane; Golgi apparatus membrane; Cell surface. In neurons, localizes to synaptic junctions. Also detected in the endoplasmic reticulum and the Golgi. Partially enriched in lipid rafts.

Topology

Extracellular: 31-423
Helical: 424-444
Cytoplasmic: 445-566

PTM

N-glycosylated.
O-glycosylated; contains chondroitin sulfate glycans. Part-time proteoglycan, expressed in part as a proteoglycan exhibiting chondroitin sulfate glycans and in part as a non-proteoglycan form. The relative amount of both forms depends on tissues and tissues maturation (By similarity).
Phosphorylated; in intracellular and extracellular parts.

Pintér, A., Hevesi, Z., Zahola, P., Alpár, A., & Hanics, J. (2020). Chondroitin sulfate proteoglycan-5 forms perisynaptic matrix assemblies in the adult rat cortex. Cellular Signalling, 74, 109710.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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