Mouse Anti-CST5 Recombinant Antibody (CBFYC-2342) (V2LY-0125-LY131)

Name: Mouse Anti-CST5 Recombinant Antibody (CBFYC-2342)
SKU: V2LY-0125-LY131
Rating: 5 (1 reviews)

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Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

CBFYC-2342

Antibody Isotype

IgG2b, κ

Application

WB, IP, IF, ELISA

Basic Information

Immunogen

Amino acids 22-53 at the N-terminus of human cystatin D.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:100-1:1,000
IP	1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium Azide

Concentration

0.2 mg/mL

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

Cystatin D

Entrez Gene ID

1473

UniProt ID

P28325

Function

Cysteine proteinase inhibitor that possibly plays a protective role against proteinases present in the oral cavity. The order of preference for inhibition is cathepsin S> cathepsin H> cathepsin L> cathepsin B.

Cellular Location

Secreted

More Infomation

References

Mohammed, N. U. G., Khaleel, F. M., & Gorial, F. I. (2021). Cystatin D as a new diagnostic marker in rheumatoid arthritis. Gene Reports, 23, 101027.

Fujita-Yoshigaki, J., Yokoyama, M., & Katsumata-Kato, O. (2020). Switching of cargo sorting from the constitutive to regulated secretory pathway by the addition of cystatin D sequence in salivary acinar cells. American Journal of Physiology-Gastrointestinal and Liver Physiology, 319(1), G74-G86.

Wang, F., Zhang, C., Ge, W., & Zhang, G. (2019). Up‐regulated CST5 inhibits bone resorption and activation of osteoclasts in rat models of osteoporosis via suppression of the NF‐κB pathway. Journal of Cellular and Molecular Medicine, 23(10), 6744-6754.

Hill, L. J., Di Pietro, V., Hazeldine, J., Davies, D., Toman, E., Logan, A., & Belli, A. (2018). Author Correction: Cystatin D (CST5): An ultra-early inflammatory biomarker of traumatic brain injury. Scientific Reports, 8(1), 1-1.

Hill, L. J., Di Pietro, V., Hazeldine, J., Davies, D., Toman, E., Logan, A., & Belli, A. (2017). Cystatin D (CST5): An ultra-early inflammatory biomarker of traumatic brain injury. Scientific Reports, 7(1), 1-10.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Related Products

Mouse Anti-CST5 Recombinant Antibody (CBYY-C2231)

Mouse Anti-CST5 Recombinant Antibody (CBWJC-3273)

Rabbit Anti-CST5 Recombinant Antibody (011)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Documents

Datasheet
SDS
Request for COA

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Second antibody and isotype control

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