Mouse Anti-CTCFL Recombinant Antibody (XB0292) (V2LY-0125-LY234)

Name: Mouse Anti-CTCFL Recombinant Antibody (XB0292)
SKU: V2LY-0125-LY234
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

XB0292

Application

ELISA, WB, IF

Immunogen

Human CTCFL.

Host Species

Mouse

Specificity

Human, Mouse

Antibody Isotype

IgG1

Clonality

Monoclonal Antibody

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Glycerol & PBS

Preservative

Sodium Azide

Concentration

Batch dependent

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

CCCTC-Binding Factor Like

Entrez Gene ID

Human	140690
Mouse	664799

UniProt ID

Human	Q8NI51
Mouse	A2APF3

Function

Testis-specific DNA binding protein responsible for insulator function, nuclear architecture and transcriptional control, which probably acts by recruiting epigenetic chromatin modifiers. Plays a key role in gene imprinting in male germline, by participating in the establishment of differential methylation at the IGF2/H19 imprinted control region (ICR). Directly binds the unmethylated H19 ICR and recruits the PRMT7 methyltransferase, leading to methylate histone H4 'Arg-3' to form H4R3sme2. This probably leads to recruit de novo DNA methyltransferases at these sites (By similarity).

Seems to act as tumor suppressor. In association with DNMT1 and DNMT3B, involved in activation of BAG1 gene expression by binding to its promoter. Required for dimethylation of H3 lysine 4 (H3K4me2) of MYC and BRCA1 promoters.

Biological Process

Cell cycle Source: UniProtKB-KW
DNA methylation involved in gamete generation Source: UniProtKB
Histone methylation Source: UniProtKB
Positive regulation of gene expression Source: UniProtKB
Positive regulation of transcription, DNA-templated Source: UniProtKB
Positive regulation of transcription by RNA polymerase II Source: NTNU_SB
Regulation of gene expression by genetic imprinting Source: UniProtKB
Regulation of histone H3-K4 methylation Source: UniProtKB
Regulation of transcription by RNA polymerase II Source: GO_Central

Cellular Location

Nucleus; Cytoplasm

Rao, G. K., Makani, V. K. K., Mendonza, J. J., Edathara, P. M., Patel, N., Ramakrishna, M., ... & Bhadra, M. P. (2021). Downregulation of BORIS/CTCFL leads to ROS‐dependent cellular senescence and drug sensitivity in MYCN‐amplified neuroblastoma. The FEBS journal.

Moscona, R., Janssen, S., Elchebly, M., Papadakis, A., Spatz, A., & Rubin, E. (2022). ATAC-Seq and RNA-Seq combined analysis elucidates the impact of BORIS/CTCFL on melanoma progression, mediated by chromatin accessibility and gene expression alterations. bioRxiv.

Chen, M. M., Zhao, R. C., Chen, K. F., Huang, Y., Liu, Z. J., Wei, Y. G., ... & Qin, Y. (2020). Hypomethylation of CTCFL promoters as a noninvasive biomarker in plasma from patients with hepatocellular carcinoma. Neoplasma, 67(04), 909-15.

Janssen, S. M., Moscona, R., Elchebly, M., Papadakis, A. I., Redpath, M., Wang, H., ... & Spatz, A. (2020). BORIS/CTCFL promotes a switch from a proliferative towards an invasive phenotype in melanoma cells. Cell death discovery, 6(1), 1-17.

Debaugny, R. E., & Skok, J. A. (2020). CTCF and CTCFL in cancer. Current opinion in genetics & development, 61, 44-52.

Gong, M., Yan, C., Jiang, Y., Meng, H., Feng, M., & Cheng, W. (2019). Genome‑wide bioinformatics analysis reveals CTCFL is upregulated in high‑grade epithelial ovarian cancer. Oncology letters, 18(4), 4030-4039.

Voutsadakis, I. A. (2018). Molecular Lesions of Insulator CTCF and Its Paralogue CTCFL (BORIS) in cancer: an analysis from published genomic studies. High-throughput, 7(4), 30.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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