Mouse Anti-CTSC Recombinant Antibody (CBYCD-135) (V2LY-0125-LY403)

Name: Mouse Anti-CTSC Recombinant Antibody (CBYCD-135)
SKU: V2LY-0125-LY403
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Published Data

Mouse Anti-CTSC Recombinant Antibody (CBYCD-135) (V2LY-0125-LY403) in WB

HT-29 cells were fractionated into cytosol and membrane fractions after DMSO or T/S/Z treatment for 4 h, followed by Western blotting with the indicated antibodies. The mature active cathepsins were shown. LDH (lactate dehydrogenase) and LAMP1 served as cytosol and membrane markers respectively. Antibody p-MLKL recognizes phospho-S358 of MLKL. ...View More

Liu, S., Perez, P., Sun, X., Chen, K., Fatirkhorani, R., Mammadova, J., & Wang, Z. (2024). MLKL polymerization-induced lysosomal membrane permeabilization promotes necroptosis. Cell Death & Differentiation, 31(1), 40-52. Distributed under Open Access license CC BY 4.0, without modification.

Mouse Anti-CTSC Recombinant Antibody (CBYCD-135) (V2LY-0125-LY403) in WB

Colors represent different UMAP clusters indicating genetically distinct NK cell subsets. Western blot of protein expression of granzyme B, cathepsin C, and perforin-1 in sNK vs. IL-2 treated primary NK cells derived from the same donor. ...View More

Kaur, K., Chen, P. C., Ko, M. W., Mei, A., Senjor, E., Malarkannan, S., ... & Jewett, A. (2023). Sequential therapy with supercharged NK cells with either chemotherapy drug cisplatin or anti-PD-1 antibody decreases the tumor size and significantly enhances the NK function in Hu-BLT mice. Frontiers in Immunology, 14, 1132807. Distributed under Open Access license CC BY 4.0, without modification.

Mouse Anti-CTSC Recombinant Antibody (CBYCD-135) (V2LY-0125-LY403) in WB

Reduced CTSC expression by HA1. Western blot bands were measured using Image J software (n = 3). ...View More

Kim, S., Lee, K. H., Choi, H. J., Kim, E., Kang, S., Han, M., ... & Lee, H. J. (2023). Hederacolchiside A1 suppresses autophagy by inhibiting cathepsin C and reduces the growth of colon cancer. Cancers, 15(4), 1272. Distributed under Open Access license CC BY 4.0, without modification.

Mouse Anti-CTSC Recombinant Antibody (CBYCD-135) (V2LY-0125-LY403) in Immunoblotting

Immunoblots for mitochondrial proteins in BMM WT, CtsB KO, CtsL KO and CtsS KO stimulated with 0.5 mM LLOMe for 1 h. (repeated three times with similar results). ...View More

Bussi, C., Heunis, T., Pellegrino, E., Bernard, E. M., Bah, N., Dos Santos, M. S., ... & Gutierrez, M. G. (2022). Lysosomal damage drives mitochondrial proteome remodelling and reprograms macrophage immunometabolism. Nature Communications, 13(1), 7338. Distributed under Open Access license CC BY 4.0, without modification.

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBYCD-135

Application

WB, IP, IF, ELISA, IHC-P

Immunogen

Amino acids 251-394 mapping mapping to the heavy chain of human cathepsin C.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:100-1:1,000
IP	1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium Azide

Concentration

0.2 mg/mL

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Cathepsin C

Entrez Gene ID

1075

UniProt ID

P53634

Function

Thiol protease. Has dipeptidylpeptidase activity. Active against a broad range of dipeptide substrates composed of both polar and hydrophobic amino acids. Proline cannot occupy the P1 position and arginine cannot occupy the P2 position of the substrate. Can act as both an exopeptidase and endopeptidase. Activates serine proteases such as elastase, cathepsin G and granzymes A and B. Can also activate neuraminidase and factor XIII.

Biological Process

Aging Source: Ensembl
COPII vesicle coating Source: Reactome
Endoplasmic reticulum to Golgi vesicle-mediated transport Source: Reactome
Immune response Source: ProtInc
Negative regulation of myelination Source: Ensembl
Neutrophil degranulation Source: Reactome
Positive regulation of apoptotic signaling pathway Source: Ensembl
Positive regulation of microglial cell activation Source: Ensembl
Positive regulation of proteolysis involved in cellular protein catabolic process Source: ParkinsonsUK-UCL
Proteolysis Source: UniProtKB
Proteolysis involved in cellular protein catabolic process Source: GO_Central
Response to organic substance Source: Ensembl
T cell mediated cytotoxicity Source: Ensembl

Cellular Location

Lysosome

PTM

N-glycosylated. While glycosylation at Asn-53, Asn-119 and Asn-276 is mediated by STT3A-containing complexes, glycosylation at Asn-29 is mediated STT3B-containing complexes.
In approximately 50% of the complexes the exclusion domain is cleaved at position 58 or 61. The two parts of the exclusion domain are held together by a disulfide bond.

Xiao, Y., Cong, M., Li, J., He, D., Wu, Q., Tian, P., ... & Hu, G. (2021). Cathepsin C promotes breast cancer lung metastasis by modulating neutrophil infiltration and neutrophil extracellular trap formation. Cancer Cell, 39(3), 423-437.

Ghanei, M., Abbaszadegan, M. R., Forghanifard, M. M., Aarabi, A., & Arab, H. (2021). A novel mutation in the cathepsin C (CTSC) gene in Iranian family with Papillon‐Lefevre syndrome. Clinical and Experimental Dental Research, 7(4), 568-573.

Zhang, G. P., Yue, X., & Li, S. Q. (2020). Cathepsin C interacts with TNF-α/p38 MAPK signaling pathway to promote proliferation and metastasis in hepatocellular carcinoma. Cancer Research and Treatment: Official Journal of Korean Cancer Association, 52(1), 10.

Khaket, T. P., Singh, M. P., Khan, I., & Kang, S. C. (2020). In vitro and in vivo studies on potentiation of curcumin-induced lysosomal-dependent apoptosis upon silencing of cathepsin C in colorectal cancer cells. Pharmacological research, 161, 105156.

John, D. S., Aschenbach, J., Krüger, B., Sendler, M., Weiss, F. U., Mayerle, J., ... & Aghdassi, A. A. (2019). Deficiency of cathepsin C ameliorates severity of acute pancreatitis by reduction of neutrophil elastase activation and cleavage of E-cadherin. Journal of Biological Chemistry, 294(2), 697-707.

Chiang, K. C., Lai, C. Y., Chiou, H. L., Lin, C. L., Chen, Y. S., Kao, S. H., & Hsieh, Y. H. (2019). Timosaponin AIII inhibits metastasis of renal carcinoma cells through suppressing cathepsin C expression by AKT/miR‐129‐5p axis. Journal of Cellular Physiology, 234(8), 13332-13341.

Alam, S., Liu, Q., Liu, S., Liu, Y., Zhang, Y., Yang, X., ... & Ma, J. (2019). Up-regulated cathepsin C induces macrophage M1 polarization through FAK-triggered p38 MAPK/NF-κB pathway. Experimental Cell Research, 382(2), 111472.

Gang Liu, Y., Teng, Y. S., Cheng, P., Kong, H., Lv, P. Y., Mao, F. Y., ... & Zhuang, A. Y. (2019). Abrogation of cathepsin C by Helicobacter pylori impairs neutrophil activation to promote gastric infection. The FASEB Journal, 33(4), 5018-5033.

Khaket, T. P., Singh, M. P., Khan, I., Bhardwaj, M., & Kang, S. C. (2018). Targeting of cathepsin C induces autophagic dysregulation that directs ER stress mediated cellular cytotoxicity in colorectal cancer cells. Cellular Signalling, 46, 92-102.

Miller, B. E., Mayer, R. J., Goyal, N., Bal, J., Dallow, N., Boyce, M., ... & Lazaar, A. L. (2017). Epithelial desquamation observed in a phase I study of an oral cathepsin C inhibitor (GSK2793660). British journal of clinical pharmacology, 83(12), 2813-2820.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

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