Mouse Anti-CUL5 Recombinant Antibody (C11339) (V2LY-0125-LY540)

Name: Mouse Anti-CUL5 Recombinant Antibody (C11339)
SKU: V2LY-0125-LY540
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Published Data

Mouse Anti-CUL5 Recombinant Antibody (C11339) (V2LY-0125-LY540) in Immunoblotting

Representative immunoblots of Cul1, Cul3, Cul4, Cul5, pSPAK, SPAK, pNCC, NCC, and P20s in isolated renal tubules that were incubated with either vehicle or the cullin inhibitor (MLN4924; 0.5 uM) for various time points (1, 2, or 4 h) ex vivo. Molecular weight markers (kDa) are shown on the right. ...View More

Murali, S. K., Little, R., Poulsen, S. B., Ferdaus, M. Z., Ellison, D. H., McCormick, J. A., & Fenton, R. A. (2021). Potassium effects on NCC are attenuated during inhibition of cullin E3-ubiquitin ligases. Cells, 11(1), 95. Distributed under Open Access license CC BY 4.0, without modification.

Mouse Anti-CUL5 Recombinant Antibody (C11339) (V2LY-0125-LY540) in WB

he in-vitro inhibition of cullin-5 neddylation. HA-9104-mesylate at indicated concentrations was incubated with a neddylation reaction mixture, containing purified E1 (UBA3/APPBP1), E2 (UBE2F), E3/substrate (SAG/CUL5), and NEDD8, followed by Western blotting. ...View More

Xu, T., Ma, Q., Li, Y., Yu, Q., Pan, P., Zheng, Y., ... & Sun, Y. (2022). A small molecule inhibitor of the UBE2F-CRL5 axis induces apoptosis and radiosensitization in lung cancer. Signal Transduction and Targeted Therapy, 7(1), 354. Distributed under Open Access license CC BY 4.0, without modification.

Mouse Anti-CUL5 Recombinant Antibody (C11339) (V2LY-0125-LY540) in Immunoblotting

HEK293T cells were co-transfected with plasmids encoding HA-p62S, shRNAs targeting CUL5, and GFP (as an internal transfection control), and were harvested for IB analysis 48 h later. Tubulin served as a loading control. ...View More

Gong, L., Wang, K., Wang, M., Hu, R., Li, H., Gao, D., & Lin, M. (2021). CUL5-ASB6 Complex Promotes p62/SQSTM1 Ubiquitination and Degradation to Regulate Cell Proliferation and Autophagy. Frontiers in Cell and Developmental Biology, 9, 684885. Distributed under Open Access license CC BY 4.0, without modification.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

C11339

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 1-300 mapping at the N-terminus of human CUL-5.

Host Species

Mouse

Specificity

Human, Mouse, Rat, Horse, Cattle, Pig

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:100-1:1,000
IP	1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium Azide

Concentration

0.2 mg/mL

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Cullin 5

Entrez Gene ID

Human	8065
Mouse	75717
Rat	64624

UniProt ID

Human	Q93034
Mouse	Q9D5V5
Rat	Q9JJ31

Function

Core component of multiple SCF-like ECS (Elongin-Cullin 2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. ECS(SOCS1) seems to direct ubiquitination of JAK2. Seems to be involved in proteosomal degradation of p53/TP53 stimulated by adenovirus E1B-55 kDa protein. May form a cell surface vasopressin receptor.

Biological Process

ERBB2 signaling pathway Source: Reactome
G1/S transition of mitotic cell cycle Source: ProtInc
Intrinsic apoptotic signaling pathway Source: ProtInc
Post-translational protein modification Source: Reactome
Protein ubiquitination Source: GO_Central
SCF-dependent proteasomal ubiquitin-dependent protein catabolic process Source: GO_Central
Viral process Source: UniProtKB-KW

Cellular Location

Cytosol; Cul5-RING ubiquitin ligase complex; Cullin-RING ubiquitin ligase complex; SCF ubiquitin ligase complex; Site of DNA damage

PTM

Neddylated; which enhances the ubiquitination activity of SCF and prevents binding of the inhibitor CAND1. Deneddylated via its interaction with the COP9 signalosome (CSN).

Bano, I., Soomro, A. S., Abbas, S. Q., Ahmadi, A., Hassan, S. S. U., Behl, T., & Bungau, S. (2022). A Comprehensive Review of Biological Roles and Interactions of Cullin-5 Protein. ACS omega.

Xu, S., Ma, Y., Tong, Q., Yang, J., Liu, J., Wang, Y., ... & Zhang, J. (2021). Cullin-5 neddylation-mediated NOXA degradation is enhanced by PRDX1 oligomers in colorectal cancer. Cell death & disease, 12(3), 1-16.

Li, Z., Hu, N., Dai, L., Hou, X., Hu, W., Liang, W., & Wang, X. (2021). Cullin-5 (CUL5) as a potential prognostic marker in a pan-cancer analysis of human tumors. Bioengineered, 12(1), 5348-5360.

Gao, F., Fan, Y., Zhou, B., Guo, W., Jiang, X., Shi, J., & Ren, C. (2020). The functions and properties of cullin-5, a potential therapeutic target for cancers. American Journal of Translational Research, 12(2), 618.

Jia, Y., Ramamoorthi, G., Kodumudi, K., Basu, A., Wiener, D., & Czerniecki, B. (2019). Th1 cytokines promotes E3 ubiquitin ligase Cullin 5 expression via STAT1 signaling cascade and enhance cul5 mediated proteasomal degradation of HER2 in HER2+/ER-breast cancer.

Si, L., Jia, Y., Lin, R., Jian, W., Yu, Q., & Yang, S. (2019). MicroRNA-27a regulates the proliferation, chemosensitivity and invasion of human ovarian cancer cell lines by targeting Cullin 5. Archives of biochemistry and biophysics, 668, 9-15.

Tapia-Laliena, M. Á., Korzeniewski, N., Peña-Llopis, S., Scholl, C., Fröhling, S., Hohenfellner, M., ... & Duensing, S. (2019). Cullin 5 is a novel candidate tumor suppressor in renal cell carcinoma involved in the maintenance of genome stability. Oncogenesis, 8(1), 1-11.

Talamantez-Lyburn, S., Brown, P., Hondrogiannis, N., Ratliff, J., Wicks, S. L., Nana, N., ... & Ehrlich, E. S. (2019). Gold nanoparticles loaded with cullin-5 DNA increase sensitivity to 17-AAG in cullin-5 deficient breast cancer cells. International journal of pharmaceutics, 564, 281-292.

Zhao, G., Gong, L., Su, D., Jin, Y., Guo, C., Yue, M., ... & Ji, H. (2019). Cullin5 deficiency promotes small-cell lung cancer metastasis by stabilizing integrin β1. The Journal of clinical investigation, 129(3), 972-987.

Gu, Q., Zhang, Z., Gertzen, C. G., Häussinger, D., Gohlke, H., & Münk, C. (2018). Identification of a conserved interface of human immunodeficiency virus type 1 and feline immunodeficiency virus Vifs with Cullin 5. Journal of virology, 92(6), e01697-17.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

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