Rabbit Anti-CYBB Recombinant Antibody (CBWJN-1491) (V2LY-0125-LY855)

Name: Rabbit Anti-CYBB Recombinant Antibody (CBWJN-1491)
SKU: V2LY-0125-LY855
Rating: 5 (1 reviews)

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Published Data

Rabbit Anti-CYBB Recombinant Antibody (CBWJN-1491) (V2LY-0125-LY855) in IF

Representative DHE staining of superoxide production in HAECs. HAECs were transfected with siControl, siKLF11, or siKLF11+siNOX2. After 48 hours, they were pretreated with NOX2 inhibitor (NOX2i, GSK2795039, 1 μM) for 1 hour, then stimulated with TNF-α (2 ng/mL) or AngII (1 μM) for 2 hours, followed by DHE staining of superoxide (red) and immunofluorescence staining of VE-cadherin (green). Nuclei stained by DAPI are shown in blue. Scale bar: 20 μm. ...View More

Zhao, G., Chang, Z., Zhao, Y., Guo, Y., Lu, H., Liang, W., ... & Zhang, J. (2021). KLF11 protects against abdominal aortic aneurysm through inhibition of endothelial cell dysfunction. JCI insight, 6(5), e141673. Distributed under Open Access license CC BY 4.0, without modification.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

CBWJN-1491

Application

Immunogen

Synthetic peptide within Human CYBB/NOX2 aa 131-180.

Host Species

Rabbit

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:2,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

BSA, Glycerol & TBS

Preservative

Sodium Azide

Concentration

1 mg/mL

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

CYBB

Entrez Gene ID

Human	1536
Mouse	13058
Rat	66021

UniProt ID

Human	P04839
Mouse	Q61093
Rat	Q9ERL1

Function

Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. Also functions as a voltage-gated proton channel that mediates the H+ currents of resting phagocytes. It participates in the regulation of cellular pH and is blocked by zinc.

Biological Process

Antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent Source: Reactome
Cell redox homeostasis Source: Reactome
Cellular response to cadmium ion Source: Ensembl
Cellular response to ethanol Source: Ensembl
Cellular response to L-glutamine Source: Ensembl
Cellular response to oxidative stress Source: Reactome
Defense response Source: GO_Central
Electron transport chain Source: GOC
Hypoxia-inducible factor-1alpha signaling pathway Source: Ensembl
Inflammatory response Source: UniProtKB
Innate immune response Source: BHF-UCL
Neutrophil degranulation Source: Reactome
Positive regulation of angiogenesis Source: Ensembl
Positive regulation of tumor necrosis factor production Source: Ensembl
Regulation of ion transmembrane transport Source: UniProtKB-KW
Respiratory burst Source: BHF-UCL
Response to aldosterone Source: Ensembl
Response to angiotensin Source: Ensembl
Response to drug Source: Ensembl
Response to nutrient Source: Ensembl
Superoxide anion generation Source: BHF-UCL
Superoxide metabolic process Source: BHF-UCL
Vascular endothelial growth factor receptor signaling pathway Source: Reactome

Cellular Location

Cell membrane. As unassembled monomer may localize to the endoplasmic reticulum.

Involvement in disease

Granulomatous disease, chronic, X-linked (CGD):
A form of chronic granulomatous disease, a primary immunodeficiency characterized by severe recurrent bacterial and fungal infections, along with manifestations of chronic granulomatous inflammation. It results from an impaired ability of phagocytes to mount a burst of reactive oxygen species in response to pathogens.
Immunodeficiency 34 (IMD34):
A form of Mendelian susceptibility to mycobacterial disease, a rare condition characterized by predisposition to illness caused by moderately virulent mycobacterial species, such as Bacillus Calmette-Guerin (BCG) vaccine, environmental non-tuberculous mycobacteria, and by the more virulent Mycobacterium tuberculosis. Other microorganisms rarely cause severe clinical disease in individuals with susceptibility to mycobacterial infections, with the exception of Salmonella which infects less than 50% of these individuals.

Topology

Cytoplasmic: 2-8
Helical: 9-29
Extracellular: 30-48
Helical: 49-69
Cytoplasmic: 70-102
Helical: 103-123
Extracellular: 124-169
Helical: 170-190
Cytoplasmic: 191-200
Helical: 201-221
Extracellular: 222-261
Helical: 262-282
Cytoplasmic: 283-570

PTM

Glycosylated.
Phosphorylated on Ser and Thr residues.
Undergoes 'Lys-48'-linked polyubiquitination, likely by RNF145, triggering endoplasmic reticulum-associated degradation.

Heydari, A., Abolnezhadian, F., Sadeghi‐Shabestari, M., Saberi, A., Shamsizadeh, A., Ghadiri, A. A., & Ghandil, P. (2021). Identification of Cytochrome b‐245, beta‐chain gene mutations, and clinical presentations in Iranian patients with X‐linked chronic granulomatous disease. Journal of Clinical Laboratory Analysis, 35(2), e23637.

Azarova, I. E., Klyosova, E. Y., Kolomoets, I. I., Azarova, V. A., Ivakin, V. E., Konoplya, A. I., & Polonikov, A. V. (2020). Polymorphisms of the Gene Encoding Cytochrome b-245 Beta Chain of NADPH Oxidase: Relationship with Redox Homeostasis Markers and Risk of Type 2 Diabetes Mellitus. Russian Journal of Genetics, 56(7), 856-862.

Gul‐Kahraman, K., Yilmaz‐Bozoglan, M., & Sahna, E. (2019). Physiological and pharmacological effects of melatonin on remote ischemic perconditioning after myocardial ischemia‐reperfusion injury in rats: Role of Cybb, Fas, NfκB, Irisin signaling pathway. Journal of Pineal Research, 67(2), e12589.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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