Mouse Anti-CYP26B1 Recombinant Antibody (2G7) (V2LY-0125-LY938)

Name: Mouse Anti-CYP26B1 Recombinant Antibody (2G7)
SKU: V2LY-0125-LY938
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

2G7

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 131-230 representing partial length CYP26B1 of human.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:100-1:1,000
IP	1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium Azide

Concentration

0.1 mg/mL

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

cytochrome P450, family 26, subfamily B, polypeptide 1

Entrez Gene ID

56603

UniProt ID

Q9NR63

Function

Involved in the metabolism of retinoic acid (RA), rendering this classical morphogen inactive through oxidation (PubMed:10823918, PubMed:22020119).

Involved in the specific inactivation of all-trans-retinoic acid (all-trans-RA), with a preference for the following substrates: all-trans-RA> 9-cis-RA> 13-cis-RA (PubMed:10823918, PubMed:22020119).

Generates several hydroxylated forms of RA, including 4-OH-RA, 4-oxo-RA, and 18-OH-RA (PubMed:10823918).

Catalyzes the hydroxylation of carbon hydrogen bonds of atRA primarily at C-4 (PubMed:10823918, PubMed:22020119).

Essential for postnatal survival (By similarity).

Plays a central role in germ cell development: acts by degrading RA in the developing testis, preventing STRA8 expression, thereby leading to delay of meiosis (By similarity).

Required for the maintenance of the undifferentiated state of male germ cells during embryonic development in Sertoli cells, inducing arrest in G0 phase of the cell cycle and preventing meiotic entry (By similarity).

Plays a role in skeletal development, both at the level of patterning and in the ossification of bone and the establishment of some synovial joints (PubMed:22019272).

Has also a significant activity in oxidation of tazarotenic acid and may therefore metabolize that xenobiotic in vivo.

Biological Process

Bone morphogenesis Source: UniProtKB
Cell fate determination Source: BHF-UCL
Cellular response to retinoic acid Source: Ensembl
Cornification Source: Ensembl
Embryonic limb morphogenesis Source: BHF-UCL
Establishment of skin barrier Source: Ensembl
Establishment of T cell polarity Source: Ensembl
Inflammatory response Source: Ensembl
Kidney development Source: Ensembl
Male meiotic nuclear division Source: BHF-UCL
Negative regulation of retinoic acid receptor signaling pathway Source: BHF-UCL
Positive regulation of gene expression Source: Ensembl
Positive regulation of tongue muscle cell differentiation Source: Ensembl
Proximal/distal pattern formation Source: BHF-UCL
Regulation of T cell differentiation Source: Ensembl
Response to vitamin A Source: Ensembl
Retinoic acid catabolic process Source: BHF-UCL
Retinoic acid metabolic process Source: UniProtKB
Retinoic acid receptor signaling pathway Source: Ensembl
Spermatogenesis Source: BHF-UCL
Sterol metabolic process Source: GO_Central
Tongue morphogenesis Source: Ensembl
Vitamin metabolic process Source: Reactome
Xenobiotic metabolic process Source: UniProtKB

Cellular Location

Endoplasmic reticulum membrane; Microsome membrane

Involvement in disease

Radiohumeral fusions with other skeletal and craniofacial anomalies (RHFCA):
A disease characterized by craniofacial malformations, occipital encephalocele, radiohumeral fusions, oligodactyly, advanced osseous maturation, and calvarial mineralization defects.

Veit, J. G., De Glas, V., Balau, B., Liu, H., Bourlond, F., Paller, A. S., ... & Diaz, P. (2021). Characterization of CYP26B1-selective inhibitor, DX314, as a potential therapeutic for keratinization disorders. Journal of Investigative Dermatology, 141(1), 72-83.

Grand, K., Skraban, C. M., Cohen, J. L., Dowsett, L., Mazzola, S., Tarpinian, J., ... & Deardorff, M. A. (2021). Nonlethal presentations of CYP26B1‐related skeletal anomalies and multiple synostoses syndrome. American Journal of Medical Genetics Part A, 185(9), 2766-2775.

Snyder, J. M., Zhong, G., Hogarth, C., Huang, W., Topping, T., LaFrance, J., ... & Isoherranen, N. (2020). Knockout of Cyp26a1 and Cyp26b1 during postnatal life causes reduced lifespan, dermatitis, splenomegaly, and systemic inflammation in mice. The FASEB Journal, 34(12), 15788-15804.

Edelsztein, N. Y., Kashimada, K., Schteingart, H. F., & Rey, R. A. (2020). CYP26B1 declines postnatally in Sertoli cells independently of androgen action in the mouse testis. Molecular Reproduction and Development, 87(1), 66-77.

Daniel, E., Barlow, H. R., Sutton, G. I., Gu, X., Htike, Y., Cowdin, M. A., & Cleaver, O. (2020). Cyp26b1 is an essential regulator of distal airway epithelial differentiation during lung development. Development, 147(4), dev181560.

Parekh, P. A., Garcia, T. X., Waheeb, R., Jain, V., Gandhi, P., Meistrich, M. L., ... & Hofmann, M. C. (2019). Undifferentiated spermatogonia regulate Cyp26b1 expression through NOTCH signaling and drive germ cell differentiation. The FASEB Journal, 33(7), 8423-8435.

Bellutti, L., Abby, E., Tourpin, S., Messiaen, S., Moison, D., Trautmann, E., ... & Livera, G. (2019). Divergent roles of CYP26B1 and endogenous retinoic acid in mouse fetal gonads. Biomolecules, 9(10), 536.

Chang, J., Zhong, R., Tian, J., Li, J., Zhai, K., Ke, J., ... & Lin, D. (2018). Exome-wide analyses identify low-frequency variant in CYP26B1 and additional coding variants associated with esophageal squamous cell carcinoma. Nature genetics, 50(3), 338-343.

Stevison, F., Hogarth, C., Tripathy, S., Kent, T., & Isoherranen, N. (2017). Inhibition of the all-trans retinoic acid (atRA) hydroxylases CYP26A1 and CYP26B1 results in dynamic, tissue-specific changes in endogenous atRA signaling. Drug Metabolism and Disposition, 45(7), 846-854.

McGurk, P. D., Swartz, M. E., Chen, J. W., Galloway, J. L., & Eberhart, J. K. (2017). In vivo zebrafish morphogenesis shows Cyp26b1 promotes tendon condensation and musculoskeletal patterning in the embryonic jaw. PLoS genetics, 13(12), e1007112.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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