Mouse Anti-CYP2R1 Recombinant Antibody (CBWJC-2107) (CBMAB-C2807WJ)

Name: Mouse Anti-CYP2R1 Recombinant Antibody (CBWJC-2107)
SKU: CBMAB-C2807WJ
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBWJC-2107

Application

WB, ELISA, IHC

Immunogen

Ovalbumin-conjugated synthetic peptide QPYLICAERR.

Host Species

Mouse

Specificity

Human, Mouse

Antibody Isotype

IgG1, λ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
ELISA	1:100-1:2,000
WB	1:100-1:2,000
IHC	1:10-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

0.02% sodium azide

Concentration

1 mg/ml

More Infomation

Target

Full Name

Cytochrome P450 Family 2 Subfamily R Member 1

Entrez Gene ID

Human	120227
Mouse	244209

UniProt ID

Human	Q6VVX0
Mouse	Q6VVW9

Function

A cytochrome P450 monooxygenase involved in activation of vitamin D precursors. Catalyzes hydroxylation at C-25 of both forms of vitamin D, vitamin D2 and D3 (calciol) (PubMed:12867411, PubMed:15465040, PubMed:18511070).

Can metabolize vitamin D analogs/prodrugs 1alpha-hydroxyvitamin D2 (doxercalciferol) and 1alpha-hydroxyvitamin D3 (alfacalcidol) forming 25-hydroxy derivatives (PubMed:15465040, PubMed:18511070).

Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase) (PubMed:12867411, PubMed:15465040, PubMed:18511070).

Biological Process

Calcitriol biosynthetic process from calciol Source: UniProtKB
Exogenous drug catabolic process Source: GO_Central
Organic acid metabolic process Source: GO_Central
Response to cesium ion Source: Ensembl
Response to ionizing radiation Source: Ensembl
Vitamin D metabolic process Source: Reactome
Vitamin metabolic process Source: Reactome
Xenobiotic metabolic process Source: GO_Central

Cellular Location

Endoplasmic reticulum membrane; Microsome membrane

Involvement in disease

Rickets vitamin D-dependent 1B (VDDR1B):
An autosomal recessive disorder caused by a selective deficiency of the active form of vitamin D (1,25-dihydroxyvitamin D3) and resulting in defective bone mineralization and clinical features of rickets. The patients sera have low calcium concentrations, low phosphate concentrations, elevated alkaline phosphatase activity and low levels of 25-hydroxyvitamin D.

Jones, P., Lucock, M., Chaplin, G., Jablonski, N. G., Veysey, M., Scarlett, C., & Beckett, E. (2020). Distribution of variants in multiple vitamin D-related loci (DHCR7/NADSYN1, GC, CYP2R1, CYP11A1, CYP24A1, VDR, RXRα and RXRγ) vary between European, East-Asian and Sub-Saharan African-ancestry populations. Genes & Nutrition, 15(1), 1-11.

Ma, X., Xie, Z., Qin, J., Luo, S., & Zhou, Z. (2020). Association of vitamin D pathway gene CYP27B1 and CYP2R1 polymorphisms with autoimmune endocrine disorders: a meta-analysis. The Journal of Clinical Endocrinology & Metabolism, 105(11), 3575-3587.

Elkhwanky, M. S., Kummu, O., Piltonen, T. T., Laru, J., Morin‐Papunen, L., Mutikainen, M., ... & Hakkola, J. (2020). Obesity represses CYP2R1, the vitamin D 25‐hydroxylase, in the liver and extrahepatic tissues. JBMR plus, 4(11), e10397.

Scazzone, C., Agnello, L., Ragonese, P., Lo Sasso, B., Bellia, C., Bivona, G., ... & Ciaccio, M. (2018). Association of CYP2R1 rs10766197 with MS risk and disease progression. Journal of neuroscience research, 96(2), 297-304.

Roizen, J. D., Casella, A., Lai, M., Long, C., Tara, Z., Caplan, I., ... & Levine, M. A. (2018). Decreased serum 25-hydroxyvitamin D in aging male mice is associated with reduced hepatic Cyp2r1 abundance. Endocrinology, 159(8), 3083-3089.

Duan, L., Xue, Z., Ji, H., Zhang, D., & Wang, Y. (2018). Effects of CYP2R1 gene variants on vitamin D levels and status: A systematic review and meta-analysis. Gene, 678, 361-369.

Manousaki, D., Dudding, T., Haworth, S., Hsu, Y. H., Liu, C. T., Medina-Gómez, C., ... & Richards, J. B. (2017). Low-frequency synonymous coding variation in CYP2R1 has large effects on vitamin D levels and risk of multiple sclerosis. The American Journal of Human Genetics, 101(2), 227-238.

Thacher, T. D., & Levine, M. A. (2017). CYP2R1 mutations causing vitamin D-deficiency rickets. The Journal of steroid biochemistry and molecular biology, 173, 333-336.

Slater, N. A., Rager, M. L., Havrda, D. E., & Harralson, A. F. (2017). Genetic variation in CYP2R1 and GC genes associated with vitamin D deficiency status. Journal of pharmacy practice, 30(1), 31-36.

Arabi, A., Khoueiry-Zgheib, N., Awada, Z., Mahfouz, R., Al-Shaar, L., Hoteit, M., ... & El Hajj Fuleihan, G. (2017). CYP2R1 polymorphisms are important modulators of circulating 25-hydroxyvitamin D levels in elderly females with vitamin insufficiency, but not of the response to vitamin D supplementation. Osteoporosis International, 28(1), 279-290.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

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