Mouse Anti-CYP2S1 Recombinant Antibody (CBWJC-3570) (V2LY-0125-LY976)

Name: Mouse Anti-CYP2S1 Recombinant Antibody (CBWJC-3570)
SKU: V2LY-0125-LY976
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBWJC-3570

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 142-300 mapping within an internal region of human CYP2S1.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:100-1:1,000
IP	1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium Azide

Concentration

0.2 mg/mL

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Cytochrome P450 Family 2 Subfamily S Member 1

Entrez Gene ID

29785

UniProt ID

Q96SQ9

Function

A cytochrome P450 monooxygenase involved in the metabolism of retinoids and eicosanoids (PubMed:12711469, PubMed:21068195).

In epidermis, may contribute to the oxidative metabolism of all-trans-retinoic acid. For this activity, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase) (PubMed:12711469).

Additionally, displays peroxidase and isomerase activities toward various oxygenated eicosanoids such as prostaglandin H2 (PGH2) and hydroperoxyeicosatetraenoates (HPETEs) (PubMed:21068195).

Independently of cytochrome P450 reductase, NADPH, and O2, catalyzes the breakdown of PGH2 to hydroxyheptadecatrienoic acid (HHT) and malondialdehyde (MDA), which is known to act as a mediator of DNA damage (PubMed:21068195).

Biological Process

Epoxygenase P450 pathway Source: GO_Central
Exogenous drug catabolic process Source: GO_Central
Icosanoid metabolic process Source: UniProtKB
Organic acid metabolic process Source: GO_Central
Prostaglandin metabolic process Source: UniProtKB
Retinoic acid metabolic process Source: UniProtKB
Xenobiotic metabolic process Source: GO_Central

Cellular Location

Endoplasmic reticulum membrane; Microsome membrane

Cabrera‐Cano, A., Dávila‐Borja, V. M., Juárez‐Méndez, S., Marcial‐Quino, J., Gómez‐Manzo, S., & Castillo‐Rodríguez, R. A. (2021). Hypoxia as a modulator of cytochromes P450: Overexpression of the cytochromes CYP2S1 and CYP24A1 in human liver cancer cells in hypoxia. Cell Biochemistry and Function, 39(4), 478-487.

Guo, H., Zeng, B., Wang, L., Ge, C., Zuo, X., Li, Y., ... & Zhang, P. (2021). Knockdown CYP2S1 inhibits lung cancer cells proliferation and migration. Cancer Biomarkers, (Preprint), 1-9.

Sheng, Y., Wen, L., Zheng, X., Li, M., Wang, D., Chen, S., ... & Zhou, F. (2021). CYP2S1 might regulate proliferation and immune response of keratinocyte in psoriasis. Epigenetics, 16(6), 618-628.

Guo, W. W., Feng, M. M., Li, S. F., & Wei, L. H. (2021). Circular RNA circ_0023404 serves as a miR‐636 sponge to promote malignant behaviors in cervical cancer cells through upregulation of CYP2S1. The Kaohsiung Journal of Medical Sciences.

Li, Y., Su, X., Feng, C., Liu, S., Guan, H., Sun, Y., ... & Hou, P. (2020). CYP2S1 is a synthetic lethal target in BRAFV600E-driven thyroid cancers. Signal transduction and targeted therapy, 5(1), 1-12.

Khor, C. Y., & Khoo, B. Y. (2020). PPARα plays an important role in the migration activity, and the expression of CYP2S1 and CYP1B1 in chrysin-treated HCT116 cells. Biotechnology letters, 42(8), 1581-1595.

Szaefer, H., Licznerska, B., Cykowiak, M., & Baer-Dubowska, W. (2019). Expression of CYP2S1 and CYP2W1 in breast cancer epithelial cells and modulation of their expression by synthetic methoxy stilbenes. Pharmacological Reports, 71(6), 1001-1005.

Yan, P., Eng, O. C., & Yu, C. J. (2018). A review on the expression and metabolic features of orphan human cytochrome P450 2S1 (CYP2S1). Current Drug Metabolism, 19(11), 917-929.

Kang, X., Shi, H., Zhang, L., Wang, Y., Zhang, T., Han, M., ... & Wang, H. (2018). Genetic polymorphisms of CYP2S1, CYP2J2 and CYP2R1 genes in three Chinese populations: Han, Tibetan and Uighur. Pharmacogenomics, 19(12), 961-977.

Singh, N. K., White, S. R., Kalagara, S., & Kadavakollu, S. (2017). Inhibition of CYP2S1 mediated metabolism of anticancer prodrug AQ4N by liarozole. Journal of Applied Pharmaceutical Science, 7(02), 001-007.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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