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Mouse Anti-CYP2W1 Recombinant Antibody (C1170) (V2LY-0125-LY980)


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Published Data
V2LY-0125-LY980-1
Mouse Anti-CYP2W1 Recombinant Antibody (C1170) (V2LY-0125-LY980) in IF
MCF-7 and MKN-45 cells were treated with 5F 203 (1 μmol/L) for 24 h, treated cells were fixed and double stained for CYP2W1 and DAPI as described above, and immunofluorescence was visualized on Nikon epifluorescence microscope. ...View More
Wang, Y., Liu, Y., Tang, T., Luo, Y., Stevens, M. F., Cheng, X., ... & Bradshaw, T. D. (2020). The antitumour activity of 2-(4-amino-3-methylphenyl)-5-fluorobenzothiazole in human gastric cancer models is mediated by AhR signalling. Journal of Cellular and Molecular Medicine, 24(2), 1750-1759. Distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
V2LY-0125-LY980-2
Mouse Anti-CYP2W1 Recombinant Antibody (C1170) (V2LY-0125-LY980) in WB
Western blot analysis of CYP2W1 expression in adrenocortical carcinoma (Ca) and normal adrenal cortex tissue (N) using the antibody 852 against a 15-residue sequence in the C-terminal end of the human CYP2W1. ...View More
Nolé, P., Duijndam, B., Stenman, A., Juhlin, C. C., Kozyra, M., Larsson, C., ... & Johansson, I. (2016). Human cytochrome P450 2W1 is not expressed in adrenal cortex and is only rarely expressed in adrenocortical carcinomas. PLoS One, 11(9), e0162379. Distributed under Open Access license CC BY 4.0, without modification.

Summary

Host Animal
Mouse
Specificity
Human
Clone
C1170
Antibody Isotype
IgG1, κ
Application
WB, IP, IF, ELISA

Basic Information

Immunogen
Amino acids 121-295 mapping within an internal region of human CYP2W1.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1:100-1:1,000
IP1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
Gelatin & PBS
Preservative
Sodium Azide
Concentration
0.2 mg/mL
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Cytochrome P450 Family 2 Subfamily W Member 1
Entrez Gene ID
54905
UniProt ID
Q8TAV3
Function
A cytochrome P450 monooxygenase that may play a role in retinoid and phospholipid metabolism (PubMed:22591743, PubMed:26936974).

Catalyzes the hydroxylation of saturated carbon hydrogen bonds. Hydroxylates all trans-retinoic acid (atRA) to 4-hydroxyretinoate and may regulate atRA clearance. Other retinoids such as all-trans retinol and all-trans retinal are potential endogenous substrates (PubMed:26936974).

Catalyzes both epoxidation of double bonds and hydroxylation of carbon hydrogen bonds of the fatty acyl chain of 1-acylphospholipids/2-lysophospholipids. Can metabolize various lysophospholipids classes including lysophosphatidylcholines (LPCs), lysophosphatidylinositols (LPIs), lysophosphatidylserines (LPSs), lysophosphatidylglycerols (LPGs), lysophosphatidylethanolamines (LPEs) and lysophosphatidic acids (LPAs) (PubMed:22591743).

Has low or no activity toward 2-acylphospholipids/1-lysophospholipids, diacylphospholipids and free fatty acids (PubMed:26936974, PubMed:22591743).

May play a role in tumorigenesis by activating procarcinogens such as aflatoxin B1, polycyclic aromatic hydrocarbon dihydrodiols and aromatic amines (PubMed:20805301, PubMed:16551781, PubMed:24278521).

Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase) (PubMed:22591743, PubMed:26936974).
Biological Process
Aflatoxin metabolic process Source: UniProtKB
Exogenous drug catabolic process Source: GO_Central
Organic acid metabolic process Source: GO_Central
Phospholipid metabolic process Source: UniProtKB
Retinoic acid catabolic process Source: UniProtKB
Xenobiotic metabolic process Source: UniProtKB
Cellular Location
Cell membrane; Endoplasmic reticulum lumen; Microsome membrane. About 8% are expressed on the cell surface.
More Infomation

Altieri, B., Sbiera, S., Herterich, S., De Francia, S., Della Casa, S., Calabrese, A., ... & Ronchi, C. L. (2020). Effects of germline CYP2W1* 6 and CYP2B6* 6 single nucleotide polymorphisms on mitotane treatment in adrenocortical carcinoma: a multicenter ENSAT study. Cancers, 12(2), 359.

Ronis, M. J., Mercer, K. E., Shankar, K., Pulliam, C., Pedersen, K., Ingelman-Sundberg, M., ... & Welsh, D. A. (2020). Potential role of gut microbiota, the proto-oncogene PIKE (Agap2) and cytochrome P450 CYP2W1 in promotion of liver cancer by alcoholic and nonalcoholic fatty liver disease and protection by dietary soy protein. Chemico-biological interactions, 325, 109131.

Szaefer, H., Licznerska, B., Cykowiak, M., & Baer-Dubowska, W. (2019). Expression of CYP2S1 and CYP2W1 in breast cancer epithelial cells and modulation of their expression by synthetic methoxy stilbenes. Pharmacological Reports, 71(6), 1001-1005.

Altieri, B., Herterich, S., Volante, M., Sbiera, S., De, F. S., Della Casa, S., ... & Ronchi, C. L. (2018, May). Germline CYP2W1* 6 polymorphism is a new predictive marker of sensitivity to mitotane treatment in advanced adrenocortical carcinoma: a multicenter European study. In Endocrine Abstracts (Vol. 56). Bioscientifica.

Altieri, B., Herterich, S., Sbiera, S., Volante, M., Kroiss, M., Della Casa, S., ... & Ronchi, C. L. (2017, May). CYP2W1* 6 polymorphism as a potential predictive marker of sensitivity to mitotane treatment in adrenocortical carcinoma. In Endocrine Abstracts (Vol. 49). Bioscientifica.

Pan, Y., & Ong, E. C. (2017). Cytochrome P450 2W1 (CYP2W1)–ready for use as the biomarker and drug target for cancer?. Xenobiotica, 47(10), 923-932.

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For research use only. Not intended for any clinical use.

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