Mouse Anti-DAB1 Recombinant Antibody (13A170) (V2LY-0125-LY1047)

Name: Mouse Anti-DAB1 Recombinant Antibody (13A170)
SKU: V2LY-0125-LY1047
Rating: 5 (1 reviews)

See all DAB1 antibodies

Online Inquiry

Published Data

Mouse Anti-DAB1 Recombinant Antibody (13A170) (V2LY-0125-LY1047) in WB

Representative western blots of pDab1 (top) and GAPDH levels (bottom) detected in the CB of both female (left) and male (right) mice either WT (WT/WT RELN), heterozygous (WT RELN/H3448R) or homozygous (RELN-H3448R/H3448R) for the mRELN-H3448R mutation. Levels of pDab1 were detected in 6-12-month-old mice. ...View More

Lopera, F., Marino, C., Chandrahas, A. S., O'Hare, M., Villalba-Moreno, N. D., Aguillon, D., ... & Quiroz, Y. T. (2023). Resilience to autosomal dominant Alzheimer's disease in a Reelin-COLBOS heterozygous man. Nature medicine, 29(5), 1243-1252. Distributed under Open Access license CC BY 4.0, without modification.

Datasheet

Summary

Host Animal

Mouse

Specificity

Human, Mouse, Rat, Horse, Dog

Clone

13A170

Antibody Isotype

IgG1, κ

Application

WB, IP, IF, ELISA

Basic Information

Immunogen

Amino acids 451-553 of human Dab1.

Host Species

Mouse

Specificity

Human, Mouse, Rat, Horse, Dog

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:100-1:1,000
IP	1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)
ELISA	1:100-1:1,000
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium Azide

Concentration

0.2 mg/mL

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Entrez Gene ID

Human	1600
Mouse	13131
Rat	266729

UniProt ID

Human	O75553
Mouse	P97318
Rat	Q8CJH2

Function

Adapter molecule functioning in neural development. May regulate SIAH1 activity.

Biological Process

Adult walking behavior Source: Ensembl
Axon guidance Source: Reactome
cell-cell adhesion involved in neuronal-glial interactions involved in cerebral cortex radial glia guided migration Source: Ensembl
Cerebellum structural organization Source: Ensembl
Dendrite development Source: Ensembl
Golgi localization Source: Ensembl
Hippocampus development Source: Ensembl
Lateral motor column neuron migration Source: GO_Central
Midgut development Source: Ensembl
Negative regulation of astrocyte differentiation Source: Ensembl
Negative regulation of axonogenesis Source: Ensembl
Negative regulation of cell adhesion Source: Ensembl
Negative regulation of receptor signaling pathway via JAK-STAT Source: Ensembl
Positive regulation of neuron differentiation Source: Ensembl
Positive regulation of protein kinase activity Source: Ensembl
Radial glia guided migration of Purkinje cell Source: Ensembl
Response to drug Source: Ensembl
Small GTPase mediated signal transduction Source: Ensembl
Ventral spinal cord development Source: GO_Central

Cellular Location

Cytosol; Apical part of cell; Brush border; Cytoplasm; Intracellular membrane-bounded organelle; Membrane; Neuron projection; Neuronal cell body; Perinuclear region of cytoplasm; Postsynaptic density

Involvement in disease

Spinocerebellar ataxia 37 (SCA37):
A form of spinocerebellar ataxia, a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA37 is an autosomal dominant form characterized by adult-onset of slowly progressive gait instability, frequent falls, and dysarthria associated with cerebellar atrophy on brain imaging.

PTM

Phosphorylated on Tyr-198 and Tyr-220 upon reelin induction in embryonic neurons. Also phosphorylated on Ser-524 independently of reelin signaling.

More Infomation

References

Chia, W., Liu, J., Huang, Y. G., & Zhang, C. (2020). A circular RNA derived from DAB1 promotes cell proliferation and osteogenic differentiation of BMSCs via RBPJ/DAB1 axis. Cell death & disease, 11(5), 1-11.

Vázquez-Carretero, M. D., García-Miranda, P., Balda, M. S., Matter, K., Peral, M. J., & Ilundain, A. A. (2018). Small and large intestine express a truncated Dab1 isoform that assembles in cell-cell junctions and co-localizes with proteins involved in endocytosis. Biochimica et Biophysica Acta (BBA)-Biomembranes, 1860(5), 1231-1241.

Gao, Z., Chen, X., Zhu, K., Zeng, P., & Ding, G. (2017). Dab1 contributes to angiotensin II-induced apoptosis via p38 signaling pathway in podocytes. BioMed research international, 2017.

Q & As

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Review & reward

Have you used Mouse Anti-DAB1 Recombinant Antibody (13A170)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Learn more

Documents

Datasheet
SDS
Request for COA

Request bulk or custom quote
Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email: