Sign in or Register   Sign in or Register
  |  

Mouse Anti-DAD1 Recombinant Antibody (2B4-C8) (CBMAB-D0173-YC)

Provided herein is a Mouse monoclonal antibody, which binds to Defender Against Cell Death 1 (DAD1). The antibody can be used for immunoassay techniques, such as ELISA.
See all DAD1 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
2B4-C8
Antibody Isotype
IgG2b, κ
Application
ELISA

Basic Information

Specificity
Human
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Defender Against Cell Death 1
Introduction
DAD1, the defender against apoptotic cell death, was initially identified as a negative regulator of programmed cell death in the temperature sensitive tsBN7 cell line. DAD1 is believed to be a tightly associated subunit of oligosaccharyltransferase both in the intact membrane and in the purified enzyme, thus reflecting the essential nature of N-linked glycosylation in eukaryotes.
Entrez Gene ID
1603
UniProt ID
P61803
Alternative Names
Defender Against Cell Death 1; Oligosaccharyltransferase Subunit 2 (Non-Catalytic); Oligosaccharyl Transferase Subunit DAD1; DAD-1; Dolichyl-Diphosphooligosaccharide--Protein Glycosyltransferase Subunit DAD1; Oligosaccharyltransferase 2 Homolog (S. Cerevisiae); Oligosaccharyltransferase 2 Homolog; EC 2.4.1.119; OST2;
Function
Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc3Man9GlcNAc2 in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation (PubMed:22467853).

N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity (By similarity).

Required for the assembly of both SST3A- and SS3B-containing OST complexes. Loss of the DAD1 protein triggers apoptosis (PubMed:22467853).
Biological Process
Apoptotic process Source: UniProtKB-KW
Blastocyst development Source: Ensembl
Negative regulation of apoptotic process Source: ProtInc
Protein glycosylation Source: UniProtKB
Protein N-linked glycosylation Source: ARUK-UCL
Protein N-linked glycosylation via asparagine Source: Reactome
Regulation of protein stability Source: ARUK-UCL
Response to drug Source: Ensembl
Response to nutrient Source: Ensembl
Viral protein processing Source: Reactome
Cellular Location
Endoplasmic reticulum membrane
Topology
Cytoplasmic: 2-30
Helical: 31-51
Lumenal: 52
Helical: 53-73
Cytoplasmic: 74-92
Helical: 93-113
Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Mouse Anti-DAD1 Recombinant Antibody (2B4-C8)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Go to
Compare