Mouse Anti-DBI Recombinant Antibody (CBFYE-0351) (V2LY-0125-LY1147)

Name: Mouse Anti-DBI Recombinant Antibody (CBFYE-0351)
SKU: V2LY-0125-LY1147
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-DBI Recombinant Antibody (CBFYE-0351) (V2LY-0125-LY1147) in WB

Western blot analysis of BmACBP protein levels after PDS injection in B. mori. (D) Values for fat body TAG accumulation. ...View More

Xiang, L., Niu, K., Peng, Y., Zhang, X., Li, X., Ye, R., ... & Feng, Q. (2022). DNA G-quadruplex structure participates in regulation of lipid metabolism through acyl-CoA binding protein. Nucleic Acids Research, 50(12), 6953-6967. Distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Mouse Anti-DBI Recombinant Antibody (CBFYE-0351) (V2LY-0125-LY1147) in IF

Representative immunofluorescence staining of G4-wild-type and G4-mutant cells for ACBP and the proliferation marker Ki67. All scale bars, 50 μm. ...View More

Mouse Anti-DBI Recombinant Antibody (CBFYE-0351) (V2LY-0125-LY1147) in Immunoblotting

Representative immunoblot images of PPARγ, ACBP, and β-actin proteins in control (shUNR) and Acbp-knocked down (shAcbp) Hep55.1c cells after treatment with vehicle or Rosi (48h), densitometric quantification (n=3). ...View More

Anagnostopoulos, G., Motiño, O., Li, S., Carbonnier, V., Chen, H., Sica, V., ... & Kroemer, G. (2022). An obesogenic feedforward loop involving PPARγ, acyl-CoA binding protein and GABAA receptor. Cell death & disease, 13(4), 356. Distributed under Open Access license CC BY 4.0, without modification.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYE-0351

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 1-87 representing full length ACBP of human.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:100-1:1,000
IP	1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)
ELISA	1:100-1:1,000
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium Azide

Concentration

0.2 mg/mL

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Diazepam Binding Inhibitor, Acyl-CoA Binding Protein

Entrez Gene ID

1622

UniProt ID

P07108

Function

Binds medium- and long-chain acyl-CoA esters with very high affinity and may function as an intracellular carrier of acyl-CoA esters. It is also able to displace diazepam from the benzodiazepine (BZD) recognition site located on the GABA type A receptor. It is therefore possible that this protein also acts as a neuropeptide to modulate the action of the GABA receptor.

Biological Process

Acyl-CoA metabolic process Source: Reactome
Negative regulation of protein lipidation Source: UniProtKB
Phosphatidylcholine acyl-chain remodeling Source: UniProtKB
Positive regulation of CoA-transferase activity Source: UniProtKB
Positive regulation of phospholipid transport Source: UniProtKB

Cellular Location

Golgi apparatus; Endoplasmic reticulum. Golgi localization is dependent on ligand binding (PubMed:17953517).

Joseph, A., Chen, H., Anagnostopoulos, G., Montégut, L., Lafarge, A., Motiño, O., ... & Kroemer, G. (2021). Effects of acyl-coenzyme A binding protein (ACBP)/diazepam-binding inhibitor (DBI) on body mass index. Cell death & disease, 12(6), 1-14.

Thomas, A. M., Asnicar, F., Kroemer, G., & Segata, N. (2021). Genes Encoding Microbial Acyl Coenzyme A Binding Protein/Diazepam-Binding Inhibitor Orthologs Are Rare in the Human Gut Microbiome and Show No Links to Obesity. Applied and environmental microbiology, 87(12), e00471-21.

Mamoor, S. (2021). Over-expression of diazepam binding inhibitor, acyl-CoA binding protein in human endometrial cancer.

Alquier, T., Christian-Hinman, C. A., Alfonso, J., & Færgeman, N. J. (2021). From benzodiazepines to fatty acids and beyond: revisiting the role of ACBP/DBI. Trends in Endocrinology & Metabolism, 32(11), 890-903.

Joseph, A., Moriceau, S., Sica, V., Anagnostopoulos, G., Pol, J., Martins, I., ... & Kroemer, G. (2020). Metabolic and psychiatric effects of acyl coenzyme A binding protein (ACBP)/diazepam binding inhibitor (DBI). Cell death & disease, 11(7), 1-10.

Charmpilas, N., Ruckenstuhl, C., Sica, V., Büttner, S., Habernig, L., Dichtinger, S., ... & Kroemer, G. (2020). Acyl-CoA-binding protein (ACBP): a phylogenetically conserved appetite stimulator. Cell death & disease, 11(1), 1-10.

Bravo-San Pedro, J. M., Sica, V., Martins, I., Anagnostopoulos, G., Maiuri, C., & Kroemer, G. (2019). Cell-autonomous, paracrine and neuroendocrine feedback regulation of autophagy by DBI/ACBP (diazepam binding inhibitor, acyl-CoA binding protein): the obesity factor. Autophagy, 15(11), 2036-2038.

Bravo-San Pedro, J. M., Sica, V., Madeo, F., & Kroemer, G. (2019). Acyl-CoA-binding protein (ACBP): the elusive ‘hunger factor'linking autophagy to food intake. Cell Stress, 3(10), 312.

Bravo-San Pedro, J. M., Sica, V., Martins, I., Pol, J., Loos, F., Maiuri, M. C., ... & Kroemer, G. (2019). Acyl-CoA-binding protein is a lipogenic factor that triggers food intake and obesity. Cell metabolism, 30(4), 754-767.

Duman, C., Yaqubi, K., Hoffmann, A., Acikgöz, A. A., Korshunov, A., Bendszus, M., ... & Alfonso, J. (2019). Acyl-CoA-binding protein drives glioblastoma tumorigenesis by sustaining fatty acid oxidation. Cell metabolism, 30(2), 274-289.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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