Mouse Anti-DBP Recombinant Antibody (8C98) (V2LY-0125-LY1161)

Name: Mouse Anti-DBP Recombinant Antibody (8C98)
SKU: V2LY-0125-LY1161
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

8C98

Application

ELISA, IF, WB, IHC

Immunogen

Human Vitamin D Binding Protein.

Host Species

Mouse

Specificity

Human, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:100-1:400
ELISA	1:100-1:200
IHC	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Glycerol & PBS

Preservative

Sodium Azide

Concentration

1 mg/mL

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

D site of albumin promoter (albumin D-box) binding protein

Entrez Gene ID

1628

UniProt ID

Q10586

Function

This transcriptional activator recognizes and binds to the sequence 5'-RTTAYGTAAY-3' found in the promoter of genes such as albumin, CYP2A4 and CYP2A5. It is not essential for circadian rhythm generation, but modulates important clock output genes. May be a direct target for regulation by the circadian pacemaker component clock. May affect circadian period and sleep regulation.

Biological Process

Circadian rhythm Source: InterPro
Liver development Source: Ensembl
Positive regulation of transcription by RNA polymerase II Source: BHF-UCL
Regulation of transcription by RNA polymerase II Source: GO_Central

Cellular Location

Nucleus

Zhao, Q., Sun, H., Yin, L., & Wang, L. (2019). miR‑126a‑5p‑Dbp and miR‑31a‑Crot/Mrpl4 interaction pairs crucial for the development of hypertension and stroke. Molecular medicine reports, 20(5), 4151-4167.

Suzuki, K., Yoshida, K., Ueha, T., Kaneshiro, K., Nakai, A., Hashimoto, N., ... & Hashiramoto, A. (2018). Methotrexate upregulates circadian transcriptional factors PAR bZIP to induce apoptosis on rheumatoid arthritis synovial fibroblasts. Arthritis research & therapy, 20(1), 1-10.

Singh, M., Bergmann, L., Lang, A., Pexa, K., Kuck, F., Stibane, D., ... & Piekorz, R. P. (2018). Gαi3 signaling is associated with sexual dimorphic expression of the clock-controlled output gene Dbp in murine liver. Oncotarget, 9(54), 30213.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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