Mouse Anti-DBR1 Recombinant Antibody (3A7) (V2LY-0125-LY1164)

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Basic Information

Host Animal
Mouse
Clone
3A7
Application
ELISA, IF, WB
Immunogen
DBR1 (445-538aa) partial recombinant protein.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
IF(ICC)10 μg/mL

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS
Preservative
None
Concentration
Batch dependent
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.
More Infomation

Target

Full Name
debranching enzyme homolog 1 (S. cerevisiae)
Entrez Gene ID
UniProt ID
Function
Cleaves the 2'-5' phosphodiester linkage at the branch point of lariat intron pre-mRNAs after splicing and converts them into linear molecules that are subsequently degraded. It thereby facilitates ribonucleotide turnover. It may also participate in retrovirus replication via an RNA lariat intermediate in cDNA synthesis.
Biological Process
mRNA splicing, via spliceosome Source: GO_Central
RNA splicing, via transesterification reactions Source: UniProtKB
Cellular Location
Nucleus

Xu, X., Yang, X., Liu, X., Bi, Y., Kong, P., Wang, Y., ... & Xi, Y. (2022). The Role of DBR1 as a Candidate Prognosis Biomarker in Esophageal Squamous Cell Carcinoma. Technology in Cancer Research & Treatment, 21, 15330338221083105.

Mohanta, A., & Chakrabarti, K. (2021). Dbr1 functions in mRNA processing, intron turnover and human diseases. Biochimie, 180, 134-142.

Valdés, J., Ortuño-Pineda, C., Saucedo-Cárdenas, O., & Mendoza-Figueroa, M. S. (2018). Unexplored Molecular Features of the Entamoeba histolytica RNA Lariat Debranching Enzyme Dbr1 Expression Profile. Frontiers in Cellular and Infection Microbiology, 8, 228.

Katolik, A., Clark, N. E., Tago, N., Montemayor, E. J., Hart, P. J., & Damha, M. J. (2017). Fluorescent Branched RNAs for High-Throughput Analysis of Dbr1 Enzyme Kinetics and Inhibition. ACS chemical biology, 12(3), 622-627.

Han, B., Park, H. K., Ching, T., Panneerselvam, J., Wang, H., Shen, Y., ... & Fei, P. (2017). Human DBR1 modulates the recycling of snRNPs to affect alternative RNA splicing and contributes to the suppression of cancer development. Oncogene, 36(38), 5382-5391.

Galvis, A. E., Fisher, H. E., Fan, H., & Camerini, D. (2017). Conformational changes in the 5′ end of the HIV-1 genome dependent on the debranching enzyme DBR1 during early stages of infection. Journal of Virology, 91(23), e01377-17.

Ransey, E., Paredes, E., Dey, S. K., Das, S. R., Heroux, A., & Macbeth, M. R. (2017). Crystal structure of the Entamoeba histolytica RNA lariat debranching enzyme EhDbr1 reveals a catalytic Zn2+/Mn2+ heterobinucleation. FEBS letters, 591(13), 2003-2010.

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For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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