Mouse Anti-DCPS Recombinant Antibody (4H8) (V2LY-0125-LY1230)

Name: Mouse Anti-DCPS Recombinant Antibody (4H8)
SKU: V2LY-0125-LY1230
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

4H8

Application

WB, IHC

Immunogen

Full length human recombinant protein of human DCPS produced in HEK293T cell

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:2,000
IHC	1:150

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

BSA, Glycerol & PBS

Preservative

Sodium Azide

Concentration

1 mg/mL

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

decapping enzyme, scavenger

Entrez Gene ID

Human	28960
Mouse	69305
Rat	266605

UniProt ID

Human	Q96C86
Mouse	Q9DAR7
Rat	Q8K4F7

Function

Decapping scavenger enzyme that catalyzes the cleavage of a residual cap structure following the degradation of mRNAs by the 3'->5' exosome-mediated mRNA decay pathway. Hydrolyzes cap analog structures like 7-methylguanosine nucleoside triphosphate (m7GpppG) with up to 10 nucleotide substrates (small capped oligoribonucleotides) and specifically releases 5'-phosphorylated RNA fragments and 7-methylguanosine monophosphate (m7GMP). Cleaves cap analog structures like tri-methyl guanosine nucleoside triphosphate (m3(2,2,7)GpppG) with very poor efficiency. Does not hydrolyze unmethylated cap analog (GpppG) and shows no decapping activity on intact m7GpppG-capped mRNA molecules longer than 25 nucleotides. Does not hydrolyze 7-methylguanosine diphosphate (m7GDP) to m7GMP (PubMed:22985415).

May also play a role in the 5'->3 mRNA decay pathway; m7GDP, the downstream product released by the 5'->3' mRNA mediated decapping activity, may be also converted by DCPS to m7GMP (PubMed:14523240).

Binds to m7GpppG and strongly to m7GDP. Plays a role in first intron splicing of pre-mRNAs. Inhibits activation-induced cell death.

Biological Process

Cellular response to menadione Source: UniProtKB
Deadenylation-dependent decapping of nuclear-transcribed mRNA Source: GO_Central
Exonucleolytic catabolism of deadenylated mRNA Source: Reactome
mRNA cis splicing, via spliceosome Source: UniProtKB
Negative regulation of programmed cell death Source: UniProtKB
Nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay Source: UniProtKB

Cellular Location

Cytoplasm; Nucleus. Predominantly localized in the nucleus. Nucleocytoplasmic shuttling protein that can transiently enter the cytoplasm in mammalian cells in a XPO1/CRM1-dependent manner.

Involvement in disease

Al-Raqad syndrome (ARS):
A syndrome characterized by delayed psychomotor development, moderate to severe intellectual disability, poor or absent speech, microcephaly, congenital hypotonia, and severe growth delay.

Salamon, I., Palsule, G., Luo, X., Roque, A., Tucai, S., Khosla, I., ... & Kiledjian, M. (2022). mRNA-decapping associated DcpS enzyme controls critical steps of neuronal development. Cerebral Cortex, 32(7), 1494-1507.

Fuchs, A. L., Wurm, J. P., Neu, A., & Sprangers, R. (2020). Molecular basis of the selective processing of short mRNA substrates by the DcpS mRNA decapping enzyme. Proceedings of the National Academy of Sciences, 117(32), 19237-19244.

Ferenc-Mrozek, A., Bojarska, E., Stepinski, J., Darzynkiewicz, E., & Lukaszewicz, M. (2020). Effect of the His-tag location on decapping scavenger enzymes and their hydrolytic activity toward cap analogs. ACS omega, 5(19), 10759-10766.

Wulf, M. G., Buswell, J., Chan, S. H., Dai, N., Marks, K., Martin, E. R., ... & Schildkraut, I. (2019). The yeast scavenger decapping enzyme DcpS and its application for in vitro RNA recapping. Scientific reports, 9(1), 1-9.

Mei, Z. Z., Sun, H., Ou, X., Li, L., Cai, J., Hu, S., ... & Jiang, Y. (2019). The natural antisense transcript NATTD regulates the transcription of decapping scavenger (DcpS) enzyme. The International Journal of Biochemistry & Cell Biology, 110, 103-110.

Pietrow, P., Ferenc-Mrozek, A., Piecyk, K., Bojarska, E., Darzynkiewicz, E., & Jankowska-Anyszka, M. (2019). Decapping Scavenger Enzyme Activity toward N2-Substituted 5′ End mRNA Cap Analogues. ACS omega, 4(17), 17576-17580.

Yamauchi, T., Masuda, T., Canver, M. C., Seiler, M., Semba, Y., Shboul, M., ... & Maeda, T. (2018). Genome-wide CRISPR-Cas9 screen identifies leukemia-specific dependence on a pre-mRNA metabolic pathway regulated by DCPS. Cancer cell, 33(3), 386-400.

Cherry, J. J., DiDonato, C. J., Androphy, E. J., Calo, A., Potter, K., Custer, S. K., ... & Tones, M. A. (2017). In vitro and in vivo effects of 2, 4 diaminoquinazoline inhibitors of the decapping scavenger enzyme DcpS: context-specific modulation of SMN transcript levels. PloS one, 12(9), e0185079.

Gopalsamy, A., Narayanan, A., Liu, S., Parikh, M. D., Kyne Jr, R. E., Fadeyi, O., ... & Jones, L. H. (2017). Design of potent mRNA decapping scavenger enzyme (DcpS) inhibitors with improved physicochemical properties to investigate the mechanism of therapeutic benefit in spinal muscular atrophy (SMA). Journal of medicinal chemistry, 60(7), 3094-3108.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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