Mouse Anti-DCTN6 Recombinant Antibody (D0420) (V2LY-0125-LY1255)

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Basic Information

Host Animal
Mouse
Clone
D0420
Application
WB, IP, IF, ELISA
Immunogen
Amino acids 18-45 near the N-terminus of human Dynactin 6.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1:100-1:1,000
IP1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
Gelatin & PBS
Preservative
Sodium Azide
Concentration
0.2 mg/mL
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.
More Infomation

Target

Full Name
Dynactin Subunit 6
Entrez Gene ID
Human10671
Mouse22428
Rat290798
UniProt ID
HumanO00399
MouseQ9WUB4
RatD4ADD8
Biological Process
Antigen processing and presentation of exogenous peptide antigen via MHC class II Source: Reactome
Endoplasmic reticulum to Golgi vesicle-mediated transport Source: Reactome
Mitotic spindle organization Source: GO_Central
Cellular Location
Cytoskeleton; Kinetochore
PTM
Phosphorylation at Thr-186 by CDK1 during mitotic prometaphase creates a binding site for PLK1 that facilitates its recruitment to kinetochores.

Sharoar, M. G., Zhou, J., Benoit, M., He, W., & Yan, R. (2021). Dynactin 6 deficiency enhances aging-associated dystrophic neurite formation in mouse brains. Neurobiology of Aging, 107, 21-29.

Fokin, A. I., David, V., Oguievetskaia, K., Derivery, E., Stone, C. E., Cao, L., ... & Gautreau, A. M. (2021). The Arp1/11 minifilament of dynactin primes the endosomal Arp2/3 complex. Science advances, 7(3), eabd5956.

Su, X., Li, H., Chen, S., & Qin, C. (2021). Study on the prognostic values of dynactin genes in low-grade glioma. Technology in Cancer Research & Treatment, 20, 15330338211010143.

Borca, M. V., Vuono, E. A., Ramirez-Medina, E., Azzinaro, P., Berggren, K. A., Singer, M., ... & Gladue, D. P. (2019). Structural glycoprotein E2 of classical swine fever virus interacts with host protein dynactin subunit 6 (DCTN6) during the virus infectious cycle. Journal of Virology, 94(1), e01642-19.

D’Aurora, M., Budani, M. C., Franchi, S., Sarra, A., Stuppia, L., Tiboni, G. M., & Gatta, V. (2019). Dynactin pathway-related gene expression is altered by aging, but not by vitrification. Reproductive Toxicology, 88, 48-55.

Wang, Q., Wang, X., Liang, Q., Wang, S., Liao, X., Li, D., & Pan, F. (2018). Prognostic value of dynactin mRNA expression in cutaneous melanoma. Medical Science Monitor: International Medical Journal of Experimental and Clinical Research, 24, 3752.

Luo, S., Jing, L., Zhao, T., Li, Y., Liu, Z., & Diao, A. (2017). Ubiquitination and dynactin regulate TMEPAI lysosomal trafficking. Scientific reports, 7(1), 1-13.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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