Mouse Anti-DCUN1D3 Recombinant Antibody (D0425) (V2LY-0125-LY1262)

Name: Mouse Anti-DCUN1D3 Recombinant Antibody (D0425)
SKU: V2LY-0125-LY1262
Rating: 5 (1 reviews)

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Datasheet

SDS

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Basic Information

Host Animal

Mouse

Clone

D0425

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 1-97 mapping at the N-terminus of human Dcun1D3.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:100-1:1,000
IP	1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium Azide

Concentration

0.2 mg/mL

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Defective In Cullin Neddylation 1 Domain Containing 3

Entrez Gene ID

Human	123879
Mouse	233805
Rat	309035

UniProt ID

Human	Q8IWE4
Mouse	Q8K0V2
Rat	Q4V8B2

Function

Contributes to the neddylation of all cullins by transfering NEDD8 from N-terminally acetylated NEDD8-conjugating E2s enzyme to different cullin C-terminal domain-RBX complexes and may play a role in the cell cycle progression by regulating the SCF ubiquitin E3 ligase complex, after UV damage (PubMed:23201271, PubMed:19617556, PubMed:27542266, PubMed:18823379).

At the cell membrane, can promote and as well inhibit cullins neddylation (PubMed:19617556, PubMed:26906416, PubMed:25349211).

Biological Process

Negative regulation of cell growth Source: UniProtKB
Negative regulation of G1/S transition of mitotic cell cycle Source: UniProtKB
Negative regulation of protein neddylation Source: UniProtKB
Positive regulation of apoptotic process Source: UniProtKB
Positive regulation of protein neddylation Source: UniProtKB
Positive regulation of ubiquitin-protein transferase activity Source: GO_Central
Post-translational protein modification Source: Reactome
Protein neddylation Source: GO_Central
Regulation of cell cycle process Source: UniProtKB
Regulation of protein neddylation Source: UniProtKB
Response to gamma radiation Source: UniProtKB
Response to UV-C Source: UniProtKB

Cellular Location

Nucleus; Cell membrane; Cytoplasm; Perinuclear region. After UVC treatment, the protein enters to the nucleus gradually (PubMed:18823379). Cell membrane localization is essential for CUL3 neddylation (PubMed:19617556).

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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