Mouse Anti-DDX47 Recombinant Antibody (CBYCD-224) (V2LY-0425-LY85)

Name: Mouse Anti-DDX47 Recombinant Antibody (CBYCD-224)
SKU: V2LY-0425-LY85
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBYCD-224

Application

WB, IP, IF, ELISA, IHC-P

Immunogen

Amino acids 395-429 near the C-terminus of human DDX47.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
IHC-P	1:50-1:500
ELISA	1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium Azide

Concentration

0.2 mg/ml

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

DEAD (Asp-Glu-Ala-Asp) box polypeptide 47

Entrez Gene ID

51202

UniProt ID

Q9H0S4

Function

Involved in apoptosis. May have a role in rRNA processing and mRNA splicing. Associates with pre-rRNA precursors.

Biological Process

Extrinsic apoptotic signaling pathway via death domain receptors Source: UniProtKB
mRNA processing Source: UniProtKB-KW
RNA splicing Source: UniProtKB
rRNA processing Source: UniProtKB

Cellular Location

Nucleolus. Localizes in the nucleolar-organizing region during ribosome biogenesis.

Wang, J. X., Ma, E. B., Zhang, J. Z., & Xing, S. P. (2022). DEAD-Box RNA Helicase DDX47 Maintains Midgut Homeostasis in Locusta migratoria. International journal of molecular sciences, 23(2), 586.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Alternative Versions

Mouse Anti-DDX47 Recombinant Antibody (CBYCD-224) (CAT#: CBMAB-D0582-YC)

Related Products

Mouse Anti-DDX47 Recombinant Antibody (CBYCD-224) (CAT#: CBMAB-D0582-YC)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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