Mouse Anti-DEFA1 Recombinant Antibody (A6D4) (CBMAB-D0670-YC)

Name: Mouse Anti-DEFA1 Recombinant Antibody (A6D4)
SKU: CBMAB-D0670-YC
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

A6D4

Application

WB, ELISA

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

defensin, alpha 1

Introduction

Defensins are a family of antimicrobial and cytotoxic peptides thought to be involved in host defense. They are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine, respiratory tract, urinary tract, and vagina. DEFA1 is found in the microbicidal granules of neutrophils and likely plays a role in phagocyte-mediated host defense. Several alpha defensin genes are clustered on chromosome 8. This gene differs from defensin, alpha 3 by only one amino acid. This gene and the gene encoding defensin, alpha 3 are both subject to copy number variation.

Entrez Gene ID

1667

UniProt ID

P59665

Alternative Names

Defensin Alpha 1; Defensin, Alpha 1, Myeloid-Related Sequence; Defensin, Alpha 2; Defensin, Alpha 1; DEFA2; HNP-1; DEF1;

Function

Defensin 1 and defensin 2 have antibacterial, fungicide and antiviral activities. Has antimicrobial activity against Gram-negative and Gram-positive bacteria. Defensins are thought to kill microbes by permeabilizing their plasma membrane.

Biological Process

Antibacterial humoral response Source: UniProtKB
Antimicrobial humoral immune response mediated by antimicrobial peptide Source: UniProtKB
Antimicrobial humoral response Source: Reactome
Cellular response to lipopolysaccharide Source: GO_Central
Chemotaxis Source: ProtInc
Defense response to fungus Source: UniProtKB-KW
Defense response to Gram-negative bacterium Source: GO_Central
Defense response to Gram-positive bacterium Source: UniProtKB
Defense response to protozoan Source: UniProtKB
Defense response to virus Source: UniProtKB-KW
Immune response Source: ProtInc
Innate immune response in mucosa Source: UniProtKB
Intracellular estrogen receptor signaling pathway Source: UniProtKB
Killing by host of symbiont cells Source: UniProtKB
Killing of cells of other organism Source: UniProtKB
Membrane disruption in other organism Source: GO_Central
Neutrophil degranulation Source: Reactome
Pore formation in membrane of other organism during symbiotic interaction Source: UniProtKB
T cell chemotaxis Source: UniProtKB

Cellular Location

Secreted

PTM

ADP-ribosylation drastically reduces cytotoxic and antibacterial activities, and enhances IL8 production.
Phosphorylation at Tyr-85 has been found in some cancer cell lines, and interferes with ADP-ribosylation.

Shrivastava, S., Chelluboina, S., Jedge, P., Doke, P., Palkar, S., Mishra, A. C., & Arankalle, V. A. (2021). Elevated Levels of Neutrophil Activated Proteins, Alpha-Defensins (DEFA1), Calprotectin (S100A8/A9) and Myeloperoxidase (MPO) Are Associated With Disease Severity in COVID-19 Patients. Frontiers in Cellular and Infection Microbiology, 1056.

Valle-Jiménez, X., Ramírez-Cosmes, A., Aquino-Domínguez, A. S., Sánchez-Peña, F., Bustos-Arriaga, J., Romero-Tlalolini, M. D. L. Á., ... & Aguilar Ruiz, S. R. (2020). Human platelets and megakaryocytes express defensin alpha 1. Platelets, 31(3), 344-354.

Chen, Q., Yang, Y., Hou, J., Shu, Q., Yin, Y., Fu, W., ... & Fang, X. (2019). Increased gene copy number of DEFA1/DEFA3 worsens sepsis by inducing endothelial pyroptosis. Proceedings of the National Academy of Sciences, 116(8), 3161-3170.

Fillerova, R., Gallo, J., Radvansky, M., Kraiczova, V., Kudelka, M., & Kriegova, E. (2017). Excellent diagnostic characteristics for ultrafast gene profiling of DEFA1-IL1B-LTF in detection of prosthetic joint infections. Journal of clinical microbiology, 55(9), 2686-2697.

Maneerat, Y., Prasongsukarn, K., Benjathummarak, S., & Dechkhajorn, W. (2017). PPBP and DEFA1/DEFA3 genes in hyperlipidaemia as feasible synergistic inflammatory biomarkers for coronary heart disease. Lipids in health and disease, 16(1), 1-12.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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