Mouse Anti-DEFA5 Recombinant Antibody (8C8) (V2LY-0425-LY156)

Name: Mouse Anti-DEFA5 Recombinant Antibody (8C8)
SKU: V2LY-0425-LY156
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

8C8

Application

ELISA, IHC, In vivo, WB

Immunogen

Recombinant Human HD5 (aa 20-94).

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal Antibody

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

None

Concentration

5 mg/ml

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Defensin Alpha 5

Entrez Gene ID

1670

UniProt ID

Q01523

Alternative Names

Defensin Alpha 5; Defensin, Alpha 5, Paneth Cell-Specific; HD5(20-94); DEF5; Defensin, Alpha 5, Preproprotein; Defensin, Alpha 5; Defensin-5; HD-5;

Function

Has antimicrobial activity against Gram-negative and Gram-positive bacteria. Defensins are thought to kill microbes by permeabilizing their plasma membrane. All DEFA5 peptides exert antimicrobial activities, but their potency is affected by peptide processing.

Biological Process

Antibacterial humoral response Source: GO_Central
Antimicrobial humoral immune response mediated by antimicrobial peptide Source: GO_Central
Antimicrobial humoral response Source: Reactome
Cellular response to lipopolysaccharide Source: GO_Central
Defense response to fungus Source: UniProtKB-KW
Defense response to Gram-negative bacterium Source: UniProtKB
defense response to Gram-positive bacterium Source: UniProtKB
Innate immune response Source: UniProtKB
Innate immune response in mucosa Source: GO_Central
Killing by host of symbiont cells Source: CACAO
Killing of cells of other organism Source: UniProtKB
Membrane disruption in other organism Source: GO_Central
Positive regulation of membrane permeability Source: UniProtKB

Cellular Location

Secreted; Secretory vesicle. Peptides HD5(20-94), HD5(23-94) and HD5(29-94) are found within tissues, HD5(20-94) being the predominant intracellular form. Peptides HD5(56-94) and HD5(63-94) are found in the extracellular milieu, HD5(63-94) being the most abundant form.

PTM

Glycosylated.

Luan, J., Ren, Y., Gao, S., & Zhang, L. (2022). High level of defensin alpha 5 in intestine may explain the low incidence of diarrhoea in COVID-19 patients. European Journal of Gastroenterology & Hepatology.

Qiao, Q., Bai, R., Song, W., Gao, H., Zhang, M., Lu, J., ... & Zhao, P. (2021). Human α-defensin 5 suppressed colon cancer growth by targeting PI3K pathway. Experimental Cell Research, 407(2), 112809.

Rana, T., Korolkova, O. Y., Rachakonda, G., Williams, A. D., Hawkins, A. T., James, S. D., ... & M’Koma, A. E. (2021). Linking bacterial enterotoxins and alpha defensin 5 expansion in the Crohn’s colitis: A new insight into the etiopathogenetic and differentiation triggers driving colonic inflammatory bowel disease. Plos one, 16(3), e0246393.

Wu, Z., Ding, Z., Cheng, B., & Cui, Z. (2021). The inhibitory effect of human DEFA5 in growth of gastric cancer by targeting BMI1. Cancer science, 112(3), 1075-1083.

Larsen, I. S., Fritzen, A. M., Carl, C. S., Agerholm, M., Damgaard, M. T. F., Holm, J. B., ... & Jensen, B. A. H. (2019). Human Paneth cell α-defensin-5 treatment reverses dyslipidemia and improves glucoregulatory capacity in diet-induced obese mice. American Journal of Physiology-Endocrinology and Metabolism, 317(1), E42-E52.

Rashidi, A., Shanley, R., Holtan, S. G., MacMillan, M. L., Yohe, S., Thyagarajan, B., ... & Weisdorf, D. J. (2018). Single Nucleotide Polymorphisms of Paneth Cell Antimicrobial Alpha Defensin-5 (DEFA5) are Associated with Acute Graft-Versus-Host Disease: Results From Analysis of BMT-CTN0201 Samples. Biology of Blood and Marrow Transplantation, 24(3), S174-S175.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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