Mouse Anti-DELE1 Recombinant Antibody (CBLY1-020) (CBMAB-K0312-LY)

This product is antibody recognizes DELE1. The antibody CBLY1-020 immunoassay techniques such as: WB, IP, IF, ELISA.
See all DELE1 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

CBLY1-020

Antibody Isotype

IgG

Application

WB, IP, IF, ELISA

Basic Information

Specificity

Human

Antibody Isotype

IgG

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

DAP3 binding cell death enhancer 1

Entrez Gene ID

9812

UniProt ID

Q14154

Alternative Names

DELE; KIAA0141

Function

DAP3-binding cell death enhancer 1:
Key activator of the integrated stress response (ISR) following mitochondrial stress (PubMed:32132706, PubMed:32132707).

In response to mitochondrial stress, cleaved by the protease OMA1, generating the DAP3-binding cell death enhancer 1 short form (DELE1(S) or S-DELE1), which translocates to the cytosol and activates EIF2AK1/HRI to trigger the ISR (PubMed:32132706, PubMed:32132707).

Essential for the induction of death receptor-mediated apoptosis through the regulation of caspase activation (PubMed:20563667).

DAP3-binding cell death enhancer 1 short form:
Protein kinase activator generated by protein cleavage in response to mitochondrial stress, which accumulates in the cytosol and specifically binds to and activates the protein kinase activity of EIF2AK1/HRI (PubMed:32132706, PubMed:32132707).

It thereby activates the integrated stress response (ISR): EIF2AK1/HRI activation promotes eIF-2-alpha (EIF2S1) phosphorylation, leading to a decrease in global protein synthesis and the induction of selected genes, including the transcription factor ATF4, the master transcriptional regulator of the ISR (PubMed:32132706, PubMed:32132707).

Biological Process

Extrinsic apoptotic signaling pathway via death domain receptors Source: UniProtKB
HRI-mediated signaling Source: UniProtKB
Integrated stress response signaling Source: UniProtKB
Regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: UniProtKB

Cellular Location

DAP3-binding cell death enhancer 1: Mitochondrion; Mitochondrion inner membrane. Associates with the mitochondrion inner membrane in response to mitochondrial stress, leading to its proteolytic processing by OMA1, and generation of the AP3-binding cell death enhancer 1 short form (DELE1(S) or S-DELE1).
DAP3-binding cell death enhancer 1 short form: Cytosol. This short form is generated by proteolytic processing by OMA1 in response to mitochondrial stress, leading to translocation to the cytosol.

PTM

DAP3-binding cell death enhancer 1:
Cleaved by OMA1 in response to mitochondrial stress, generating the DAP3-binding cell death enhancer 1 short form (DELE1(S) or S-DELE1) that accumulates in the cytosol and activates the protein kinase activity of EIF2AK1/HRI (PubMed:32132706, PubMed:32132707). Protein cleavage by OMA1 can take place at different positions, and apparently does not require a specific sequence motif (PubMed:32132707).

References

Fessler, E., Krumwiede, L., & Jae, L. T. (2022). DELE1 tracks perturbed protein import and processing in human mitochondria. Nature Communications, 13(1), 1-15.

Cheng, M., Yu, H., Kong, Q., Wang, B., Shen, L., Dong, D., & Sun, L. (2022). The Mitochondrial PHB2/OMA1/DELE1 Pathway Cooperates with Endoplasmic Reticulum Stress to Facilitate the Response to Chemotherapeutics in Ovarian Cancer. International Journal of Molecular Sciences, 23(3), 1320.

Sekine, Y., Houston, R., Fessler, E., Jae, L. T., Narendra, D. P., & Sekine, S. (2022). A mitochondrial iron-sensing pathway regulated by DELE1. bioRxiv.

Sui, L., Zeng, J., Ye, L., Sanders, A. J., Zhao, H., Jiang, A., ... & Jiang, W. G. (2021). PTH-100 DAP3 and the DAP3 Binding Cell Death Enhancer-1 (DELE1) in human colorectal cancer.

Suskiewicz, M. J., Zobel, F., Ogden, T. E., Fontana, P., Ariza, A., Yang, J. C., ... & Ahel, I. (2020). A pathway coordinated by DELE1 relays mitochondrial stress to the cytosol.

Guo, X., Aviles, G., Liu, Y., Tian, R., Unger, B. A., Lin, Y. H. T., ... & Kampmann, M. (2019). Mitochondrial dysfunction is signaled to the integrated stress response by OMA1, DELE1 and HRI. bioRxiv, 715896.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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