Mouse Anti-DGKI Recombinant Antibody (CBYCD-255) (CBMAB-D0792-YC)

Name: Mouse Anti-DGKI Recombinant Antibody (CBYCD-255)
SKU: CBMAB-D0792-YC
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBYCD-255

Application

Immunogen

E. coli-derived recombinant human DGK-iota, Ser925-Val1065 (predicted), Accession # O75912

Specificity

Human, Mouse

Antibody Isotype

IgG2a

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Diacylglycerol Kinase Iota

Introduction

DGKI is a member of the type IV diacylglycerol kinase subfamily. Diacylglycerol kinases regulate the intracellular concentration of diacylglycerol through its phosphorylation, producing phosphatidic acid. The specific role of the enzyme encoded by this gene is undetermined, however, it may play a crucial role in the production of phosphatidic acid in the retina or in recessive forms of retinal degeneration.

Entrez Gene ID

Human	9162
Mouse	320127

UniProt ID

Human	O75912
Mouse	D3YWQ0

Alternative Names

Diacylglycerol Kinase Iota; Diglyceride Kinase Iota; DAG Kinase Iota; EC 2.7.1.107; DGK-IOTA;

Function

Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids (PubMed:9830018, PubMed:23949095).

Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes (Probable). Has probably no preference for any of the diacylglycerols in terms of the acyl chain composition, especially for the acyl chain at the sn-2 position (PubMed:9830018).

By controlling the diacylglycerol/DAG-mediated activation of RASGRP3, negatively regulates the Rap1 signaling pathway. May play a role in presynaptic diacylglycerol/DAG signaling and control neurotransmitter release during metabotropic glutamate receptor-dependent long-term depression (By similarity).

Biological Process

Diacylglycerol metabolic process Source: GO_Central
Intracellular signal transduction Source: GO_Central
Lipid phosphorylation Source: BHF-UCL
Platelet activation Source: Reactome
Protein kinase C-activating G protein-coupled receptor signaling pathway Source: InterPro
Regulation of synaptic transmission, glutamatergic Source: GO_Central

Cellular Location

Synaptic cell membrane; Nucleus; Cytosol; Axon; Dendrite; Presynapse; Postsynapse; Postsynaptic density; Synaptic vesicle membrane. Excluded from inhibitory synapses (By similarity). Localization between cytoplasm and nucleus is regulated by protein kinase C (PubMed:9830018). Both in the detergent soluble and particulate fractions (By similarity).

Arranz-Nicolás, J., & Mérida, I. (2020). Biological regulation of diacylglycerol kinases in normal and neoplastic tissues: new opportunities for cancer immunotherapy. Advances in Biological Regulation, 75, 100663.

Bartsch, V. B., Lord, J. S., Diering, G. H., & Zylka, M. J. (2020). Mania‐and anxiety‐like behavior and impaired maternal care in female diacylglycerol kinase eta and iota double knockout mice. Genes, Brain and Behavior, 19(3), e12570.

Huang, C., Zhao, J., Luo, C., & Zhu, Z. (2020). Overexpression of DGKI in gastric cancer predicts poor prognosis. Frontiers in medicine, 320.

Bartsch, V. B., Niehaus, J. K., Taylor-Blake, B., & Zylka, M. J. (2019). Enhanced histamine-induced itch in diacylglycerol kinase iota knockout mice. PloS one, 14(6), e0217819.

Ariff, A., Melton, P. E., Brennecke, S. P., & Moses, E. K. (2019). Analysis of the epigenome in multiplex pre-eclampsia families identifies SORD, DGKI, and ICA1 as novel candidate risk genes. Frontiers in genetics, 10, 227.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Mouse Anti-DGKI Recombinant Antibody (CBYCD-255) (CAT#: V2LY-0425-LY250)

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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