Mouse Anti-DGLUCY Recombinant Antibody (1949) (CBMAB-1949CQ)

Name: Mouse Anti-DGLUCY Recombinant Antibody (1949)
SKU: CBMAB-1949CQ
Rating: 5 (1 reviews)

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Summary

Host Animal

Mouse

Specificity

Mouse, Rat

Clone

1949

Antibody Isotype

IgG1, κ

Application

WB, IP, IF, ELISA

Basic Information

Immunogen

Amino acids 207-234 within an internal region of mouse DGLUCY.

Host Species

Mouse

Specificity

Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium Azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

D-Glutamate Cyclase

Introduction

DGLUCY (D-Glutamate Cyclase) is a Protein Coding gene. Among its related pathways are D-Glutamine and D-glutamate metabolism. D-glutamate cyclase that converts D-glutamate to 5-oxo-D-proline.

Entrez Gene ID

Mouse	217830
Rat	362769

UniProt ID

Mouse	Q8BH86
Rat	D4A9W3

Alternative Names

9030617O03Rik; D-glutamate cyclase, mitochondrial; UPF0317 protein C14orf159 homolog, mitochondrial; EC 4.2.1.48

Function

D-glutamate cyclase that converts D-glutamate to 5-oxo-D-proline.

Biological Process

Glutamate metabolic process Source: UniProtKB

Cellular Location

Mitochondrion matrix

More Infomation

References

Katane, M., Motoda, R., Ariyoshi, M., Tateishi, S., Nakayama, K., Saitoh, Y., ... & Homma, H. (2020). A colorimetric assay method for measuring d-glutamate cyclase activity. Analytical Biochemistry, 605, 113838.

Katane, M., Kuwabara, H., Nakayama, K., Saitoh, Y., Miyamoto, T., Sekine, M., & Homma, H. (2020). Biochemical characterization of d-aspartate oxidase from Caenorhabditis elegans: Its potential use in the determination of free d-glutamate in biological samples. Biochimica et Biophysica Acta (BBA)-Proteins and Proteomics, 1868(8), 140442.

Katane, M., Ariyoshi, M., Tateishi, S., Koiwai, S., Takaku, K., Nagai, K., ... & Homma, H. (2018). Structural and enzymatic properties of mammalian d-glutamate cyclase. Archives of biochemistry and biophysics, 654, 10-18.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Learn more

Documents

Datasheet
SDS
Request for COA

Request bulk or custom quote Online Inquiry
Second antibody and isotype control

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