Mouse Anti-DHH Recombinant Antibody (D0827) (V2LY-0425-LY274)

Name: Mouse Anti-DHH Recombinant Antibody (D0827)
SKU: V2LY-0425-LY274
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

D0827

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 227-311 of human Dhh.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
ELISA	1:100-1:1,000
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium Azide

Concentration

0.2 mg/ml

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

desert hedgehog homolog (Drosophila)

Entrez Gene ID

Human	50846
Mouse	13363
Rat	84380

UniProt ID

Human	O43323
Mouse	Q61488
Rat	G3V7Y0

Function

Intercellular signal essential for a variety of patterning events during development. May function as a spermatocyte survival factor in the testes. Essential for testes development.

Biological Process

Cell-cell signaling Source: InterPro
Cell fate specification Source: GO_Central
Leydig cell differentiation Source: Ensembl
Male sex determination Source: Ensembl
Myelination Source: Ensembl
Osteoblast differentiation Source: Ensembl
Protein autoprocessing Source: InterPro
Regulation of gene expression Source: GO_Central
Regulation of steroid biosynthetic process Source: Ensembl
Response to estradiol Source: Ensembl
Response to estrogen Source: Ensembl
Smoothened signaling pathway Source: GO_Central
Spermatid development Source: Ensembl

Cellular Location

Desert hedgehog protein N-product: Cell membrane. The N-terminal peptide remains associated with the cell surface.
Desert hedgehog protein C-product: Extracellular space. The C-terminal peptide diffuses from the cell.

Involvement in disease

Partial gonadal dysgenesis with minifascicular neuropathy 46,XY (PGD):
Characterized by the presence of a testis on one side and a streak or an absent gonad at the other, persistence of Muellerian duct structures, and a variable degree of genital ambiguity.
46,XY sex reversal 7 (SRXY7):
A disorder of sex development. Affected individuals have a 46,XY karyotype but present as phenotypically normal females. SRXY7 patients have no functional gonads.

PTM

The C-terminal domain displays an autoproteolysis activity and a cholesterol transferase activity. Both activities result in the cleavage of the full-length protein and covalent attachment of a cholesterol moiety to the C-terminal of the newly generated N-terminal fragment (N-product). This covalent modification appears to play an essential role in restricting the spatial distribution of the protein activity to the cell surface. The N-product is the active species in both local and long-range signaling, whereas the C-product has no signaling activity (By similarity).

Chapouly, C., Hollier, P. L., Guimbal, S., Cornuault, L., Gadeau, A. P., & Renault, M. A. (2020). Desert hedgehog-driven endothelium integrity is enhanced by gas1 (growth arrest-specific 1) but negatively regulated by Cdon (cell adhesion molecule-related/downregulated by oncogenes). Arteriosclerosis, Thrombosis, and Vascular Biology, 40(12), e336-e349.

Moreau, N., & Boucher, Y. (2020). Hedging against neuropathic pain: role of hedgehog signaling in pathological nerve healing. International Journal of Molecular Sciences, 21(23), 9115.

Pession, A., Lonetti, A., Bertuccio, S., Locatelli, F., & Masetti, R. (2019). Targeting Hedgehog pathway in pediatric acute myeloid leukemia: challenges and opportunities. Expert Opinion on Therapeutic Targets, 23(2), 87-91.

Sasai, N., Toriyama, M., & Kondo, T. (2019). Hedgehog signal and genetic disorders. Frontiers in genetics, 1103.

Abramyan, J. (2019). Hedgehog signaling and embryonic craniofacial disorders. Journal of developmental biology, 7(2), 9.

Skoda, A. M., Simovic, D., Karin, V., Kardum, V., Vranic, S., & Serman, L. (2018). The role of the Hedgehog signaling pathway in cancer: A comprehensive review. Bosnian journal of basic medical sciences, 18(1), 8.

Riaz, S. K., Khan, J. S., Shah, S. T. A., Wang, F., Ye, L., Jiang, W. G., & Malik, M. F. A. (2018). Involvement of hedgehog pathway in early onset, aggressive molecular subtypes and metastatic potential of breast cancer. Cell Communication and Signaling, 16(1), 1-12.

Fattahi, S., Pilehchian Langroudi, M., & Akhavan‐Niaki, H. (2018). Hedgehog signaling pathway: Epigenetic regulation and role in disease and cancer development. Journal of Cellular Physiology, 233(8), 5726-5735.

Akyala, A. I., & Peppelenbosch, M. P. (2018). Gastric cancer and Hedgehog signaling pathway: emerging new paradigms. Genes & cancer, 9(1-2), 1.

Jäger, T., Ocker, M., Kiesslich, T., Neureiter, E., & Neureiter, D. (2017). Thoughts on investigational hedgehog pathway inhibitors for the treatment of cancer. Expert Opinion on Investigational Drugs, 26(2), 133-136.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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