Mouse Anti-DHX32 Recombinant Antibody (CBYCD-268) (CBMAB-D0851-YC)

Provided herein is a Mouse monoclonal antibody, which binds to DEAH-Box Helicase 32 (Putative) (DHX32). The antibody can be used for immunoassay techniques, such as ELISA.
See all DHX32 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

CBYCD-268

Antibody Isotype

IgG2a, κ

Application

ELISA

Basic Information

Immunogen

Partial recombinant (a.a.645-744) from human DHX32 (NP_060650) with GST tag.

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

DEAH (Asp-Glu-Ala-His) box polypeptide 32

Introduction

DHX32 is a member of DEAD box family. The function of this member has not been determined. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division.

Entrez Gene ID

55760

UniProt ID

Q7L7V1

Alternative Names

DEAH-Box Helicase 32 (Putative); DEAD/H (Asp-Glu-Ala-Asp/His) Box Polypeptide 32; DEAH (Asp-Glu-Ala-His) Box Polypeptide 32; DEAH Box Protein 32; DEAD/H Box 32; HuDDX32; DDX32;

Cellular Location

Nucleus; Mitochondrion

References

Wei, Q., Geng, J., Chen, Y., Lin, H., Wang, J., Fang, Z., ... & Zhang, Z. (2021). Structure and function of DEAH-box helicase 32 and its role in cancer. Oncology Letters, 21(5), 1-8.

Hu, X., Yuan, G., Li, Q., Huang, J., Cheng, X., & Chen, J. (2021). DEAH-box polypeptide 32 promotes hepatocellular carcinoma progression via activating the β-catenin pathway. Annals of Medicine, 53(1), 437-447.

Cai, M. J., Zhu, J. H., He, J. Q., Zhang, Z. Y., & Liang, X. M. (2020). Silencing of DHX32 increases the proliferation of liver cancer cells. TRANSLATIONAL CANCER RESEARCH, 9(3), 1833-1842.

Wang, M., Zhang, G., Wang, Y., Ma, R., Zhang, L., Lv, H., ... & Kang, X. (2017). DHX32 expression is an indicator of poor breast cancer prognosis. Oncology letters, 13(2), 942-948.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Related Products

Mouse Anti-DHX32 Recombinant Antibody (2G4)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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