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Mouse Anti-DIO1 Recombinant Antibody (1E4) (CBMAB-D0933-YC)

Provided herein is a Mouse monoclonal antibody, which binds to Iodothyronine Deiodinase 1 (DIO1). The antibody can be used for immunoassay techniques, such as ELISA.
See all DIO1 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
1E4
Antibody Isotype
IgG2a, κ
Application
ELISA

Basic Information

Immunogen
DIO1 (NP_000783.2, 35 a.a. ~ 125 a.a) partial recombinant protein with GST tag.
Specificity
Human
Antibody Isotype
IgG2a, κ
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
deiodinase, iodothyronine, type I
Introduction
DIO1 belongs to the iodothyronine deiodinase family. It catalyzes the activation, as well as the inactivation of thyroid hormone by outer and inner ring deiodination, respectively. The activation reaction involves the conversion of the prohormone thyroxine (3,5,3',5'-tetraiodothyronine, T4), secreted by the thyroid gland, to the bioactive thyroid hormone (3,5,3'-triiodothyronine, T3) by 5'-deiodination. This protein is expressed predominantly in the liver and kidney and provides most of the circulating T3, which is essential for growth, differentiation and basal metabolism in vertebrates. This protein is a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Alternatively spliced transcript variants have been found for this gene.
Entrez Gene ID
1733
UniProt ID
P49895
Alternative Names
Iodothyronine Deiodinase 1; Deiodinase, Iodothyronine Type I; Type I Iodothyronine Deiodinase; Thyroxine 5-Deiodinase; Type-I 5-Deiodinase; Type 1 DI; TXDI1; DIOI;
Function
Responsible for the deiodination of T4 (3,5,3',5'-tetraiodothyronine) into T3 (3,5,3'-triiodothyronine) and of T3 into T2 (3,3'-diiodothyronine). Plays a role in providing a source of plasma T3 by deiodination of T4 in peripheral tissues such as liver and kidney.
Biological Process
Cellular amino acid metabolic process Source: ARUK-UCL
Hormone biosynthetic process Source: UniProtKB-KW
Thyroid hormone generation Source: ProtInc
Thyroid hormone metabolic process Source: GO_Central
Cellular Location
Endoplasmic reticulum membrane
Topology
Helical: 13-33

França, M. M., German, A., Fernandes, G. W., Liao, X. H., Bianco, A. C., Refetoff, S., & Dumitrescu, A. M. (2021). Human type 1 iodothyronine deiodinase (DIO1) mutations cause abnormal thyroid hormone metabolism. Thyroid, 31(2), 202-207.

França, M. M., Liao, X. H., Fernandes, G. W., German, A., Bianco, A. C., Refetoff, S., & Dumitrescu, A. (2020). OR01-01 human type 1 iodothyronine deiodinase (DIO1) mutations cause abnormal thyroid hormone metabolism. Journal of the Endocrine Society, 4(Supplement_1), OR01-01.

Gogoi, P., & Kalita, J. C. (2020). Effects of butylparaben exposure on thyroid peroxidase (TPO) and type 1 iodothyronine deiodinase (D1) in female Wistar rats. Toxicology, 443, 152562.

Qian, B., Wang, C., Zhao, C., Jiang, R., & Song, J. (2019). Effects of maternal exposure to BDE209 on neuronal development and transcription of iodothyronine deiodinase in offspring mice. Toxicology mechanisms and methods, 29(8), 569-579.

Olker, J. H., Korte, J. J., Denny, J. S., Hartig, P. C., Cardon, M. C., Knutsen, C. N., ... & Hornung, M. W. (2019). Screening the ToxCast phase 1, phase 2, and e1k chemical libraries for inhibitors of iodothyronine deiodinases. Toxicological Sciences, 168(2), 430-442.

Hou, Z., Sun, Q., Hu, Y., Yang, S., Zong, Y., & Zhao, R. (2018). Maternal betaine administration modulates hepatic type 1 iodothyronine deiodinase (Dio1) expression in chicken offspring through epigenetic modifications. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 218, 30-36.

Young Cho, Y., Jeong Kim, H., Won Jang, H., Hyuk Kim, T., Ki, C. S., Wook Kim, S., & Hoon Chung, J. (2017). The relationship of 19 functional polymorphisms in iodothyronine deiodinase and psychological well-being in hypothyroid patients. Endocrine, 57(1), 115-124.

Poplawski, P., Rybicka, B., Boguslawska, J., Rodzik, K., Visser, T. J., Nauman, A., & Piekielko-Witkowska, A. (2017). Induction of type 1 iodothyronine deiodinase expression inhibits proliferation and migration of renal cancer cells. Molecular and Cellular Endocrinology, 442, 58-67.

Popławski, P., Wiśniewski, J. R., Rijntjes, E., Richards, K., Rybicka, B., Köhrle, J., & Piekiełko-Witkowska, A. (2017). Restoration of type 1 iodothyronine deiodinase expression in renal cancer cells downregulates oncoproteins and affects key metabolic pathways as well as anti-oxidative system. PloS one, 12(12), e0190179.

Llop, S., Murcia, M., Alvarez-Pedrerol, M., Grimalt, J. O., Santa-Marina, L., Julvez, J., ... & Lopez-Espinosa, M. J. (2017). Association between exposure to organochlorine compounds and maternal thyroid status: role of the iodothyronine deiodinase 1 gene. Environment international, 104, 83-90.

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For research use only. Not intended for any clinical use.

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