Mouse Anti-DLK2 Recombinant Antibody (CBYC-P056) (CBMAB-D1067-YC)

Name: Mouse Anti-DLK2 Recombinant Antibody (CBYC-P056)
SKU: CBMAB-D1067-YC
Rating: 5 (1 reviews)

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Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

CBYC-P056

Antibody Isotype

IgG3, κ

Application

WB, IP, IF, ELISA

Basic Information

Immunogen

Amino acids 250-271 within an extracellular domain of human DLK2.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG3, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium Azide

Concentration

0.2 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

Delta Like Non-Canonical Notch Ligand 2

Introduction

DLK2 (Delta Like Non-Canonical Notch Ligand 2) is a protein coding gene. Among its related pathways are Canonical and Non-canonical Notch signaling and P38 MAPK Signaling Pathway (sino). It has proved that the protein has calcium ion binding and protein heterodimerization activity. And a paralog is DLK1.

Entrez Gene ID

65989

UniProt ID

Q6UY11

Alternative Names

Delta Like Non-Canonical Notch Ligand 2; Epidermal Growth Factor-Like Protein 9; EGF-Like-Domain, Multiple 9; EGF-Like Protein 9; EGFL9; DLK-2; Delta-Like 2 Homolog (Drosophila); Protein Delta Homolog 2; Delta-Like 2 Homolog;

Function

Regulates adipogenesis.

Biological Process

Negative regulation of Notch signaling pathway Source: HGNC
Regulation of fat cell differentiation Source: Ensembl

Cellular Location

Membrane

Topology

Extracellular: 27-306
Helical: 307-327
Cytoplasmic: 328-383

More Infomation

References

Naranjo, A. I., González-Gómez, M. J., Baladrón, V., Laborda, J., & Nueda, M. L. (2022). Different Expression Levels of DLK2 Inhibit NOTCH Signaling and Inversely Modulate MDA-MB-231 Breast Cancer Tumor Growth In Vivo. International Journal of Molecular Sciences, 23(3), 1554.

Ashcroft, A. (2019). Comparative functional analysis of the atypical Notch ligands DLK1 and DLK2 in vertebrate development (Doctoral dissertation, University of Cambridge).

Navarrete, F., García-Gutiérrez, M. S., Laborda, J., & Manzanares, J. (2017). Deletion of Dlk2 increases the vulnerability to anxiety-like behaviors and impairs the anxiolytic action of alprazolam. Psychoneuroendocrinology, 85, 134-141.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Learn more

Documents

Datasheet
SDS
Request for COA

Request bulk or custom quote Online Inquiry
Second antibody and isotype control

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