Mouse Anti-DNAAF1 Recombinant Antibody (2C4) (V2LY-0425-LY600)

Name: Mouse Anti-DNAAF1 Recombinant Antibody (2C4)
SKU: V2LY-0425-LY600
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

2C4

Application

WB, FC

Immunogen

Full length human recombinant protein of human LRRC50 produced in HEK293T cell.

Host Species

Mouse

Specificity

Human, Mouse, Rat, Dog, Monkey

Antibody Isotype

IgG1

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000
FC	1:100

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

BSA & Glycerol & PBS

Preservative

Sodium Azide

Concentration

Batch dependent

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Dynein Axonemal Assembly Factor 1

Entrez Gene ID

Human	123872
Mouse	68270
Rat	361419
Dog	479628

UniProt ID

Human	Q8NEP3
Mouse	Q9D2H9
Rat	Q6AYH9
Dog	F6URB8

Function

Cilium-specific protein required for the stability of the ciliary architecture. Plays a role in cytoplasmic preassembly of dynein arms. Involved in regulation of microtubule-based cilia and actin-based brush border microvilli.

Biological Process

Axonemal dynein complex assembly Source: UniProtKB
Cilium assembly Source: UniProtKB
Cilium movement Source: BHF-UCL
Determination of digestive tract left/right asymmetry Source: BHF-UCL
Determination of liver left/right asymmetry Source: BHF-UCL
Determination of pancreatic left/right asymmetry Source: BHF-UCL
Epithelial cilium movement involved in determination of left/right asymmetry Source: BHF-UCL
Heart looping Source: BHF-UCL
Inner dynein arm assembly Source: BHF-UCL
Left/right pattern formation Source: BHF-UCL
Lung development Source: BHF-UCL
Motile cilium assembly Source: BHF-UCL
Outer dynein arm assembly Source: BHF-UCL
Regulation of cilium beat frequency Source: BHF-UCL

Cellular Location

Cytoplasm; Spindle pole; Cilium. In HEK293T cells, it is diffusely cytoplasmic and concentrates at the mitotic spindle poles, while in MDCK cells, it localizes in the cilium. In vivo, this protein is probably restricted to the cilium.

Involvement in disease

Ciliary dyskinesia, primary, 13 (CILD13):
A disorder characterized by abnormalities of motile cilia. Respiratory infections leading to chronic inflammation and bronchiectasis are recurrent, due to defects in the respiratory cilia; reduced fertility is often observed in male patients due to abnormalities of sperm tails. Half of the patients exhibit randomization of left-right body asymmetry and situs inversus, due to dysfunction of monocilia at the embryonic node. Primary ciliary dyskinesia associated with situs inversus is referred to as Kartagener syndrome. At ultrastructural level, CILD13 is characterized by a marked reduction or absence of both dynein arms from the patients cilia.

Wang, Y., Liu, Z., Yang, G., Gao, Q., Xiao, L., Li, J., ... & Zhang, H. (2020). Coding variants coupled with rapid modeling in Zebrafish implicate dynein genes, dnaaf1 and zmynd10, as adolescent idiopathic scoliosis candidate genes. Frontiers in cell and developmental biology, 1218.

Hartill, V. L., van de Hoek, G., Patel, M. P., Little, R., Watson, C. M., Berry, I. R., ... & Johnson, C. A. (2018). DNAAF1 links heart laterality with the AAA+ ATPase RUVBL1 and ciliary intraflagellar transport. Human molecular genetics, 27(3), 529-545.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Mouse Anti-DNAAF1 Recombinant Antibody (2C4) (CAT#: CBMAB-L0313-YJ)

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Mouse Anti-DNAAF1 Recombinant Antibody (3A1) (CAT#: V2LY-0425-LY598)

Mouse Anti-DNAAF1 Recombinant Antibody (2C4) (CAT#: CBMAB-L0313-YJ)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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