Mouse Anti-DNAJC10 Recombinant Antibody (3C4) (V2LY-0425-LY645)

Name: Mouse Anti-DNAJC10 Recombinant Antibody (3C4)
SKU: V2LY-0425-LY645
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-DNAJC10 Recombinant Antibody (3C4) (V2LY-0425-LY645-1) in WB

Site 2 (a) and site 3 (b) peptides indicated by TANGO were inserted into the ER-Cλ-QVT construct and co-expressed with either GRP170, ERdj5, or ERdj4-3HA. ...View More

Pobre-Piza, K. F., Mann, M. J., Flory, A. R., & Hendershot, L. M. (2022). Mapping SP-C co-chaperone binding sites reveals molecular consequences of disease-causing mutations on protein maturation. Nature Communications, 13(1), 1821.

Mouse Anti-DNAJC10 Recombinant Antibody (3C4) (V2LY-0425-LY645-2) in Immunoblot

24 h after the cotransfection of Myc-tagged Ero1α and FLAG-tagged ERdj5s into HEK293T cells, cell lysates were prepared for immunoprecipitation with anti-FLAG antibody. The immunoprecipitates were subjected to reducing SDS-PAGE before immunoblotting with the indicated antibodies. ...View More

Uegaki, K., Tokunaga, Y., Inoue, M., Takashima, S., Inaba, K., Takeuchi, K., ... & Nagata, K. (2023). The oxidative folding of nascent polypeptides provides electrons for reductive reactions in the ER. Cell Reports, 42(7).

Datasheet

Summary

Host Animal

Mouse

Specificity

Human, Mouse, Rat

Clone

3C4

Antibody Isotype

IgG1, κ

Application

WB, IHC-P, IF, ELISA

Basic Information

Immunogen

DNAJC10 (aa688-793) partial recombinant protein.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
IF(ICC)	10 µg/ml
IHC-P	3 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

None

Concentration

Batch dependent

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

DnaJ (Hsp40) homolog, subfamily C, member 10

Entrez Gene ID

Human	54431
Mouse	66861
Rat	295690

UniProt ID

Human	Q8IXB1
Mouse	Q9DC23
Rat	Q498R3

Function

Endoplasmic reticulum disulfide reductase involved both in the correct folding of proteins and degradation of misfolded proteins. Required for efficient folding of proteins in the endoplasmic reticulum by catalyzing the removal of non-native disulfide bonds formed during the folding of proteins, such as LDLR. Also involved in endoplasmic reticulum-associated degradation (ERAD) by reducing incorrect disulfide bonds in misfolded glycoproteins recognized by EDEM1. Interaction with HSPA5 is required its activity, not for the disulfide reductase activity, but to facilitate the release of DNAJC10 from its substrate. Promotes apoptotic signaling pathway in response to endoplasmic reticulum stress.

Biological Process

Intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress Source: UniProtKB
Negative regulation of protein phosphorylation Source: UniProtKB
Positive regulation of ATPase activity Source: UniProtKB
Protein folding in endoplasmic reticulum Source: UniProtKB
Response to endoplasmic reticulum stress Source: UniProtKB
Ubiquitin-dependent ERAD pathway Source: UniProtKB

Cellular Location

Endoplasmic reticulum lumen

More Infomation

References

Liu, F., Tu, Z., Liu, J., Long, X., Xiao, B., Fang, H., ... & Zhu, X. (2022). DNAJC10 correlates with tumor immune characteristics and predicts the prognosis of glioma patients. Bioscience Reports, 42(1), BSR20212378.

Acun, T., & Senses, K. M. (2020). Downregulation of DNAJC10 (ERDJ5) is associated with poor survival in breast cancer. Breast Cancer, 27(3), 483-489.

Shi, B., Shao, B., Yang, C., Guo, Y., Fu, X., & Gan, N. (2019). Upregulation of JHDM1D-AS1 protects PDLSCs from H2O2-induced apoptosis by decreasing DNAJC10 via phosphorylation of eIF2α. Biochimie, 165, 48-56.

Kimura, M., Tamura, Y., Guignabert, C., Takei, M., Kosaki, K., Tanabe, N., ... & Sano, M. (2017). A genome-wide association analysis identifies PDE1A| DNAJC10 locus on chromosome 2 associated with idiopathic pulmonary arterial hypertension in a Japanese population. Oncotarget, 8(43), 74917.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Alternative Versions

Mouse Anti-DNAJC10 Recombinant Antibody (3C4)

Related Products

Mouse Anti-DNAJC10 Recombinant Antibody (3C4)

Mouse Anti-DnaJC10 Recombinant Antibody (D1307)

Mouse Anti-DnaJC10 Recombinant Antibody (E-5)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Documents

Datasheet
SDS
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Second antibody and isotype control

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