Mouse Anti-DTX3 Recombinant Antibody (4F10) (CBMAB-D1851-YC)

Provided herein is a Mouse monoclonal antibody, which binds to Deltex E3 Ubiquitin Ligase 3 (DTX3). The antibody can be used for immunoassay techniques, such as ELISA.
See all DTX3 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

4F10

Antibody Isotype

IgG3, λ

Application

ELISA

Basic Information

Immunogen

DTX3 (NP_848597, 201 a.a. ~ 300 a.a) partial recombinant protein with GST tag. The immunogen sequence: MCGRFYGQLV GNQPQNGRML VSKDATLLLP SYEKYGTIVI QYVFPPGVQG AEHPNPGVRY PGTTRVAYLP DCPEGNKVLT LFRKAFDQRL TFTIGTSMTT

Specificity

Human

Antibody Isotype

IgG3, λ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

aa 201-300

Target

Full Name

deltex 3 homolog (Drosophila)

Introduction

DTX3 functions as an E3 ubiquitin ligase.

Entrez Gene ID

196403

UniProt ID

Q8N9I9

Alternative Names

RNF154; deltex3

Research Area

Regulator of Notch signaling, a signaling pathway involved in cell-cell communications that regulates a broad spectrum of cell-fate determinations. Probably acts both as a positive and negative regulator of Notch, depending on the developmental and cell context (By similarity).

Functions as an ubiquitin ligase protein in vitro, suggesting that it may regulate the Notch pathway via some ubiquitin ligase activity.

Biological Process

Notch signaling pathway Source: GO_Central
Protein ubiquitination Source: GO_Central

Cellular Location

Cytoplasm

References

Xu, H., Liang, S., Hu, J., Liu, W., Dong, Z., & Wei, S. (2022). Deltex E3 ubiquitin ligase 3 inhibits colorectal cancer cell growth and regulates cell cycle progression via upregulating E2F transcription factor 1. Molecular Biology Reports, 49(3), 1661-1668.

Wang, S., Hao, Q., Li, J., Chen, Y., Lu, H., Wu, X., & Zhou, X. (2020). Ubiquitin ligase DTX3 empowers mutant p53 to promote ovarian cancer development. Genes & Diseases.

Ding, X. Y., Hu, H. Y., Huang, K. N., Wei, R. Q., Min, J., Qi, C., ... & Qin, X. (2020). Ubiquitination of NOTCH2 by DTX3 suppresses the proliferation and migration of human esophageal carcinoma. Cancer science, 111(2), 489-501.

Liu, J., Li, H., Mao, A., Lu, J., Liu, W., Qie, J., & Pan, G. (2020). DCAF13 promotes triple-negative breast cancer metastasis by mediating DTX3 mRNA degradation. Cell Cycle, 19(24), 3622-3631.

Chatrin, C., Gabrielsen, M., Buetow, L., Nakasone, M. A., Ahmed, S. F., Sumpton, D., ... & Huang, D. T. (2020). Structural insights into ADP-ribosylation of ubiquitin by Deltex family E3 ubiquitin ligases. Science advances, 6(38), eabc0418.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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