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Mouse Anti-DTX3L Recombinant Antibody (1D10) (CBMAB-D1852-YC)

Provided herein is a Mouse monoclonal antibody, which binds to Deltex E3 Ubiquitin Ligase 3L (DTX3L). The antibody can be used for immunoassay techniques, such as ELISA, WB.
See all DTX3L antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
1D10
Antibody Isotype
IgG2a, κ
Application
ELISA, WB

Basic Information

Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Deltex E3 Ubiquitin Ligase 3L
Introduction
DTX3L functions as an E3 ubiquitin ligase.
Entrez Gene ID
151636
UniProt ID
Q8TDB6
Alternative Names
Deltex E3 Ubiquitin Ligase 3L; RING-Type E3 Ubiquitin Transferase DTX3L; B-Lymphoma- And BAL-Associated Protein; Rhysin-2; Rhysin2; BBAP; Deltex 3 Like, E3 Ubiquitin Ligase; E3 Ubiquitin-Protein Ligase DTX3L;
Research Area
E3 ubiquitin-protein ligase which, in association with ADP-ribosyltransferase PARP9, plays a role in DNA damage repair and in interferon-mediated antiviral responses (PubMed:12670957, PubMed:19818714, PubMed:26479788, PubMed:23230272).

Monoubiquitinates several histones, including histone H2A, H2B, H3 and H4 (PubMed:28525742).

In response to DNA damage, mediates monoubiquitination of 'Lys-91' of histone H4 (H4K91ub1) (PubMed:19818714).

The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 'Lys-20' methylation (H4K20me) (PubMed:19818714).

PARP1-dependent PARP9-DTX3L-mediated ubiquitination promotes the rapid and specific recruitment of 53BP1/TP53BP1, UIMC1/RAP80, and BRCA1 to DNA damage sites (PubMed:23230272).

By monoubiquitinating histone H2B H2BC9/H2BJ and thereby promoting chromatin remodeling, positively regulates STAT1-dependent interferon-stimulated gene transcription and thus STAT1-mediated control of viral replication (PubMed:26479788).

Independently of its catalytic activity, promotes the sorting of chemokine receptor CXCR4 from early endosome to lysosome following CXCL12 stimulation by reducing E3 ligase ITCH activity and thus ITCH-mediated ubiquitination of endosomal sorting complex required for transport ESCRT-0 components HGS and STAM (PubMed:24790097).

In addition, required for the recruitment of HGS and STAM to early endosomes (PubMed:24790097).

In association with PARP9, plays a role in antiviral responses by mediating 'Lys-48'-linked ubiquitination of encephalomyocarditis virus (EMCV) and human rhinovirus (HRV) C3 proteases and thus promoting their proteosomal-mediated degradation (PubMed:26479788).
Biological Process
Cellular response to DNA damage stimulus Source: UniProtKB
Defense response to virus Source: UniProtKB-KW
Double-strand break repair Source: UniProtKB
Endosome to lysosome transport Source: UniProtKB
Histone H2A ubiquitination Source: UniProtKB
Histone H2B ubiquitination Source: UniProtKB
Histone monoubiquitination Source: UniProtKB
Innate immune response Source: UniProtKB-KW
Negative regulation of ubiquitin-protein transferase activity Source: UniProtKB
Notch signaling pathway Source: GO_Central
Positive regulation of chromatin binding Source: UniProtKB
Positive regulation of defense response to virus by host Source: UniProtKB
Positive regulation of double-strand break repair via nonhomologous end joining Source: UniProtKB
Positive regulation of NAD+ ADP-ribosyltransferase activity Source: UniProtKB
Positive regulation of protein binding Source: UniProtKB
Positive regulation of protein localization to early endosome Source: UniProtKB
Positive regulation of protein localization to nucleus Source: UniProtKB
Positive regulation of receptor catabolic process Source: UniProtKB
Positive regulation of transcription, DNA-templated Source: UniProtKB
Protein autoubiquitination Source: UniProtKB
Protein K48-linked ubiquitination Source: UniProtKB
Protein transport Source: UniProtKB-KW
Protein ubiquitination Source: GO_Central
Ubiquitin-dependent protein catabolic process Source: UniProtKB
Cellular Location
Nucleus; Lysosome membrane; Cytoplasm; Early endosome membrane. Translocates to the nucleus in response to IFNG or IFNB1 stimulation (PubMed:26479788). Localizes at sites of DNA damage in a PARP1-dependent manner (PubMed:23230272). Localization to early endosomes is increased upon CXCL12 stimulation where it co-localizes with ITCH, CXCL4, HGS and STAM (PubMed:24790097). A minor proportion localizes to lysosomes (PubMed:24790097).
PTM
Autoubiquitinated.
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For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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