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Mouse Anti-DYRK3 Recombinant Antibody (3E10) (CBMAB-D2028-YC)

Provided herein is a Mouse monoclonal antibody, which binds to Dual Specificity Tyrosine Phosphorylation Regulated Kinase 3 (DYRK3). The antibody can be used for immunoassay techniques, such as ELISA, IHC.
See all DYRK3 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
3E10
Antibody Isotype
IgG1, κ
Application
ELISA, IHC

Basic Information

Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 3
Introduction
DYRK3 belongs to the DYRK family of dual-specificity protein kinases that catalyze autophosphorylation on serine/threonine and tyrosine residues. The members of this family share structural similarity, however, differ in their substrate specificity, suggesting their involvement in different cellular functions. The encoded protein has been shown to autophosphorylate on tyrosine residue and catalyze phosphorylation of histones H3 and H2B in vitro. Alternatively spliced transcript variants encoding different isoforms have been identified.
Entrez Gene ID
8444
UniProt ID
O43781
Alternative Names
Dual Specificity Tyrosine Phosphorylation Regulated Kinase 3; Regulatory Erythroid Kinase; Dual-Specificity Tyrosine-(Y)-Phosphorylation Regulated Kinase 5; Dual Specificity Tyrosine-(Y)-Phosphorylation Regulated Kinase 3; Protein Kinase Dyrk3; EC 2.7.12.1; REDK;
Research Area
Dual-specificity protein kinase that promotes disassembly of several types of membraneless organelles during mitosis, such as stress granules, nuclear speckles and pericentriolar material (PubMed:29973724).

Dual-specificity tyrosine-regulated kinases (DYRKs) autophosphorylate a critical tyrosine residue in their activation loop and phosphorylate their substrate on serine and threonine residues (PubMed:9748265, PubMed:29634919).

Acts as a central dissolvase of membraneless organelles during the G2-to-M transition, after the nuclear-envelope breakdown: acts by mediating phosphorylation of multiple serine and threonine residues in unstructured domains of proteins, such as SRRM1 and PCM1 (PubMed:29973724).

Does not mediate disassembly of all membraneless organelles: disassembly of P-body and nucleolus is not regulated by DYRK3 (PubMed:29973724).

Dissolution of membraneless organelles at the onset of mitosis is also required to release mitotic regulators, such as ZNF207, from liquid-unmixed organelles where they are sequestered and keep them dissolved during mitosis (PubMed:29973724).

Regulates mTORC1 by mediating the dissolution of stress granules: during stressful conditions, DYRK3 partitions from the cytosol to the stress granule, together with mTORC1 components, which prevents mTORC1 signaling (PubMed:23415227).

When stress signals are gone, the kinase activity of DYRK3 is required for the dissolution of stress granule and mTORC1 relocation to the cytosol: acts by mediating the phosphorylation of the mTORC1 inhibitor AKT1S1, allowing full reactivation of mTORC1 signaling (PubMed:23415227).

Also acts as a negative regulator of EPO-dependent erythropoiesis: may place an upper limit on red cell production during stress erythropoiesis (PubMed:10779429).

Inhibits cell death due to cytokine withdrawal in hematopoietic progenitor cells (PubMed:10779429).

Promotes cell survival upon genotoxic stress through phosphorylation of SIRT1: this in turn inhibits p53/TP53 activity and apoptosis (PubMed:20167603).
Biological Process
Cell cycle Source: UniProtKB-KW
Cell division Source: UniProtKB-KW
Erythrocyte differentiation Source: UniProtKB
Negative regulation of apoptotic process Source: Ensembl
Negative regulation of DNA damage response, signal transduction by p53 class mediator Source: Ensembl
Nuclear speck organization Source: UniProtKB
Organelle disassembly Source: UniProtKB
Peptidyl-serine phosphorylation Source: GO_Central
Peptidyl-threonine phosphorylation Source: GO_Central
Positive regulation of cell cycle G2/M phase transition Source: UniProtKB
Protein phosphorylation Source: UniProtKB
Regulation of cellular response to stress Source: UniProtKB
Regulation of TORC1 signaling Source: UniProtKB
Stress granule disassembly Source: UniProtKB
Cellular Location
Cytoplasm; Nucleus; Nucleus speckle; Centrosome; Cytoplasmic granule. Associates with membraneless organelles in the cytoplasm and nucleus (PubMed:29973724). Shuttles between cytoplasm and stress granules (PubMed:20167603). Localized predominantly on distinct speckles distributed throughout the cytoplasm of the cell (PubMed:20167603). At low concentration, showns a homogeneous distribution throughout the cytoplasm and does not condense in speckles. During oxidative and osmotic stress, localizes to stress granules (PubMed:20167603).
PTM
Ubiquitinated at anaphase by the anaphase-promoting complex (APC/C), leading to its degradation by the proteasome.
Protein kinase activity is activated following autophosphorylation at Tyr-369 (Probable). Autophosphorylation at Ser-350 stabilizes the protein and enhances the protein kinase activity (PubMed:9748265).
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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