Rabbit Anti-EBOV NP Recombinant Antibody (CBMW-H1421) (V2LY-0425-LY1292)

Name: Rabbit Anti-EBOV NP Recombinant Antibody (CBMW-H1421)
SKU: V2LY-0425-LY1292
Rating: 5 (1 reviews)

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Published Data

Rabbit Anti-EBOV NP Recombinant Antibody (CBMW-H1421) (V2LY-0425-LY1292-1) in WB

EBOV virus-like particles (VLPs) were produced from HEK293T WT, CALR or CANX-overexpressing, and CALR- or CANX-KO cells after expression of EBOV-GP1,2 with EBOV-VP40. VLPs were purified by ultra-centrifugation and protein expression in cell lysate and virions was analyzed by WB. ...View More

Zhang, J., Wang, B., Gao, X., Peng, C., Shan, C., Johnson, S. F., ... & Zheng, Y. H. (2022). RNF185 regulates proteostasis in Ebolavirus infection by crosstalk between the calnexin cycle, ERAD, and reticulophagy. Nature communications, 13(1), 6007.

Rabbit Anti-EBOV NP Recombinant Antibody (CBMW-H1421) (V2LY-0425-LY1292-2) in WB

EBOV virus-like particles (VLPs) were produced from 293T cells after expressing EBOV VP40, human MARCH8 or its W114A mutant, and EBOV GP or GPΔMLD. After purification via ultracentrifugation, these VLPs were analyzed by WB. ...View More

Yu, C., Li, S., Zhang, X., Khan, I., Ahmad, I., Zhou, Y., ... & Zheng, Y. H. (2020). MARCH8 inhibits Ebola virus glycoprotein, human immunodeficiency virus type 1 envelope glycoprotein, and avian influenza virus H5N1 hemagglutinin maturation. MBio, 11(5), 10-1128.

Rabbit Anti-EBOV NP Recombinant Antibody (CBMW-H1421) (V2LY-0425-LY1292-3) in WB

EBOV GP and L antagonize HERC5. 293T cells were co-transfected with plasmids carrying FLAG-tagged VP40 and either empty vector or HERC5 and one plasmid carrying either EBOV VP30, VP35, L NP, GP or VSV-G. Forty-eight hours post-transfection, VP40 protein levels in cell lysates and VLPs released into supernatant were analyzed by quantitative Western blotting and quantified densitometrically after normalization to GAPDH levels ...View More

Paparisto, E., Hunt, N. R., Labach, D. S., Coleman, M. D., Di Gravio, E. J., Dodge, M. J., ... & Barr, S. D. (2021). Interferon-induced HERC5 inhibits Ebola virus particle production and is antagonized by Ebola glycoprotein. Cells, 10(9), 2399.

Rabbit Anti-EBOV NP Recombinant Antibody (CBMW-H1421) (V2LY-0425-LY1292-4) in WB

Western blot analysis of EBOV immunogens.
Subunit (rEBOVGP, EBOV-VLP), vector (VSVΔG/EBOVGP, Ad5/EBOVGP) immunogens and 293T cell lysates transfected with plasmid expressing EBOV GP (pCAGGS/EBOVGP) were separated by SDS-PAGE under reducing conditions, transferred to PVDF membrane, and detected using EBOV GP1-specific mAb. Lane NC is 293T cell lysates transfected with pCAGGS plasmid. ...View More

Zhang, Y., Wei, Y., Li, Y., Wang, X., Liu, Y., Tian, D., ... & Yang, L. (2021). IgY antibodies against Ebola virus possess post-exposure protection in a murine pseudovirus challenge model and excellent thermostability. PLoS Neglected Tropical Diseases, 15(3), e0008403.

Datasheet

SDS

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Basic Information

Host Animal

Rabbit

Clone

CBMW-H1421

Application

ELISA, WB

Immunogen

Recombinant Ebola virus nucleoprotein protein.

Host Species

Rabbit

Specificity

Ebola Virus

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
ELISA	1:5,000-1:10,000
WB	1:1,000-1:5,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

None

Concentration

Batch dependent

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Ebola virus - Mayinga, Zaire, 1976 Nucleoprotein

Research Area

Oligomerizes into helical capsid to encapsidate the viral genome, protecting it from nucleases and the cellular innate immune response (PubMed:26119732, PubMed:25865894, PubMed:30333622, PubMed:16719918).

VP35 binds to and stabilizes monomeric NP, keeping it soluble (PubMed:26119732, PubMed:25865894).

Upon virus replication, NP is recruited to bind cooperatively viral genomic RNA and VP35 is released (PubMed:29144446).

The encapsidated genomic RNA is termed the nucleocapsid and serves as template for transcription and replication. The nucleocapsid is helical with a pitch of 10.81 NP per turn and a diameter of about 22nm (PubMed:22247782).

Each NP binds to six nucleotides of viral genomic RNA, three being exposed to the solvant and three hidden into the nucleocapsid (PubMed:30333622).

Recruits also host PPP2R5C phosphatase to dephosphorylate VP30 and thereby promote viral transcription (PubMed:29290611, PubMed:27755595).

Upon virion assembly and budding, NP binds to VP24 and possibly host STAU1 (PubMed:28794491, PubMed:30301857).

Biological Process

Viral RNA genome packaging Source: InterPro

PTM

Phosphorylated by host.
O-glycosylated by host.
Acetylated by host EP300 in vitro.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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