Mouse Anti-EIF2S3 Recombinant Antibody (1H3) (V2LY-0425-LY1685)

Name: Mouse Anti-EIF2S3 Recombinant Antibody (1H3)
SKU: V2LY-0425-LY1685
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

1H3

Application

WB, ELISA

Immunogen

EIF2S3 (aa383-472) partial recombinant protein.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal Antibody

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

None

Concentration

Batch dependent

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

eukaryotic translation initiation factor 2, subunit 3 gamma, 52kDa

Entrez Gene ID

1968

UniProt ID

P41091

Research Area

As a subunit of eukaryotic initiation factor 2 (eIF-2), involved in the early steps of protein synthesis. In the presence of GTP, eIF-2 forms a ternary complex with initiator tRNA Met-tRNAi and then recruits the 40S ribosomal complex and initiation factors eIF-1, eIF-1A and eIF-3 to form the 43S pre-initiation complex (43S PIC), a step that determines the rate of protein translation. The 43S PIC binds to mRNA and scans downstream to the initiation codon, where it forms a 48S initiation complex by codon-anticodon base pairing. This leads to the displacement of eIF-1 to allow GTPase-activating protein (GAP) eIF-5-mediated hydrolysis of eIF2-bound GTP. Hydrolysis of GTP and release of Pi, which makes GTP hydrolysis irreversible, causes the release of the eIF-2-GDP binary complex from the 40S subunit, an event that is essential for the subsequent joining of the 60S ribosomal subunit to form an elongation-competent 80S ribosome. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must be exchanged with GTP by way of a reaction catalyzed by GDP-GTP exchange factor (GEF) eIF-2B (By similarity).

Along with its paralog on chromosome Y, may contribute to spermatogenesis up to the round spermatid stage (By similarity).

Biological Process

Formation of translation preinitiation complex Source: GO_Central
Positive regulation of translational fidelity Source: GO_Central
Translational initiation Source: UniProtKB

Cellular Location

Cytosol; Extracellular exosome; Cytoplasm; Eukaryotic translation initiation factor 2 complex

Involvement in disease

MEHMO syndrome (MEHMO):
An X-linked recessive mental retardation syndrome characterized by intellectual disability, epileptic seizures, hypogonadism and hypogenitalism, microcephaly, and obesity.

Ivanova, N., Serzhanova, V., Demina, N., Guseva, D., & Skoblov, M. (2022). mRNA analysis revealed a novel pathogenic EIF2S3 variant causing MEHMO syndrome. European Journal of Medical Genetics, 65(2), 104421.

Moortgat, S., Manfroid, I., Pendeville, H., Freeman, S., Bourdouxhe, J., Benoit, V., ... & Maystadt, I. (2021). Broadening the phenotypic spectrum and physiological insights related to EIF2S3 variants. Human Mutation, 42(7), 827-834.

Söylemez, Z., Arikan, E. S., Solak, M., Arikan, Y., Tokyol, Ç., & Şeker, H. (2021). Investigation of the expression levels of CPEB4, APC, TRIP13, EIF2S3, EIF4A1, IFNg, PIK3CA and CTNNB1 genes in different stage colorectal tumors. Turkish Journal of Medical Sciences, 51(2), 661-674.

Kotzaeridou, U., Young‐Baird, S. K., Suckow, V., Thornburg, A. G., Wagner, M., Harting, I., ... & Kalscheuer, V. M. (2020). Novel pathogenic EIF2S3 missense variants causing clinically variable MEHMO syndrome with impaired eIF2γ translational function, and literature review. Clinical genetics, 98(5), 507-514.

Rohozinski, J., & Edwards, C. L. (2020). Does EIF2S3 Retrogene Activation Regulate Cancer/Testis Antigen Expression in Human Cancers?. Frontiers in Oncology, 10, 590408.

SÖylemez, Z., Arikan, E. S., Solak, M., Arikan, Y., Tokyol, Ç., & Şeker, H. (2020). Investigation of the expression levels of CPEB4, APC, TRIP13, EIF2S3, EIF4A, IFNgamma, PIK3CA and CTNNB1 genes in different stage colorectal tumors. Turkish Journal of Medical Sciences.

Gregory, L. C., Ferreira, C. B., Young-Baird, S. K., Williams, H. J., Harakalova, M., van Haaften, G., ... & Dattani, M. T. (2019). Impaired EIF2S3 function associated with a novel phenotype of X-linked hypopituitarism with glucose dysregulation. EBioMedicine, 42, 470-480.

Young-Baird, S. K., Shin, B. S., & Dever, T. E. (2019). MEHMO syndrome mutation EIF2S3-I259M impairs initiator Met-tRNAiMet binding to eukaryotic translation initiation factor eIF2. Nucleic Acids Research, 47(2), 855-867.

Stanik, J., Skopkova, M., Stanikova, D., Brennerova, K., Barak, L., Ticha, L., ... & Gasperikova, D. (2018). Neonatal hypoglycemia, early-onset diabetes and hypopituitarism due to the mutation in EIF2S3 gene causing MEHMO syndrome. Physiol Res, 67(2), 331-337.

Lu, J., Xu, L., Luo, X., Chen, S., Zou, Y., & Tan, H. (2018). The Expression of EIF2S3 in Acute Myeloid Leukemia and Its Prognostic Significance.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Mouse Anti-EIF2S3 Recombinant Antibody (1H3) (CAT#: CBMAB-A2529-LY)

Related Products

Mouse Anti-EIF2S3 (AA 383-472) Recombinant Antibody (CBFYE-0670) (CAT#: CBMAB-E1087-FY)

Mouse Anti-EIF2S3 Recombinant Antibody (CBFYE-0670) (CAT#: V2LY-0425-LY1684)

Mouse Anti-EIF2S3 Recombinant Antibody (1H3) (CAT#: CBMAB-A2529-LY)

Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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