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Mouse Anti-EIF3A Recombinant Antibody (CBFYE-0672) (CBMAB-E1090-FY)

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Published Data
Mouse Anti-EIF3A Recombinant Antibody (CBFYE-0672) (CBMAB-E1090-FY-1) in IF
Mouse Anti-EIF3A Recombinant Antibody (CBFYE-0672) (CBMAB-E1090-FY-1) in IF
Visualization of biotinylated proteins and eIF3A by immunofluorescence. HCT-116 (A) and HEK-293T (B) cells were seeded on glass slides, fixed and stained for biotinylated proteins and eIF3A by streptavidine-fluorescein conjugate or indirect immunofluorescence, respectively, as described in the "Materials and Methods" section. Nuclei were counterstained by DAPI. Scale bar indicates 50 µm. ...View More
Vo, D. K., Engler, A., Stoimenovski, D., Hartig, R., Kaehne, T., Kalinski, T., ... & Nass, N. (2021). Interactome Mapping of EIF3A in a Colon Cancer and an Immortalized Embryonic Cell Line Using Proximity-Dependent Biotin Identification. Cancers, 13(6), 1293.
Mouse Anti-EIF3A Recombinant Antibody (CBFYE-0672) (CBMAB-E1090-FY-2) in WB
Mouse Anti-EIF3A Recombinant Antibody (CBFYE-0672) (CBMAB-E1090-FY-2) in WB
Biotinylation of proteins by myc-BirA* and myc-BirA*-eIF3A in HEK-293T and HCT-116 cells. Cells were transfected with the plasmids and incubated with biotin for the indicated time. Crude lysate and streptavidin-affinity purified proteins were analyzed by Western blot, demonstrating the presence of biotinylated proteins (A) and eIF3A (B) as well as the myc-BirA*-eIF3A fusion protein. ...View More
Vo, D. K., Engler, A., Stoimenovski, D., Hartig, R., Kaehne, T., Kalinski, T., ... & Nass, N. (2021). Interactome Mapping of EIF3A in a Colon Cancer and an Immortalized Embryonic Cell Line Using Proximity-Dependent Biotin Identification. Cancers, 13(6), 1293.
Datasheet
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Basic Information

Host Animal
Mouse
Clone
CBFYE-0672
Application
ELISA, IF, WB, IP, IHC-P
Immunogen
Amino acids 1-300 mapping at the N-terminus of human eIF3θ.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal Antibody
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
ELISA1:100-1:1,000
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
IHC-P1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
Gelatin & PBS
Preservative
Sodium Azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
More Infomation

Target

Full Name
Eukaryotic Translation Initiation Factor 3 Subunit A
Introduction
EIF3A (Eukaryotic Translation Initiation Factor 3 Subunit A) is a Protein Coding gene. Diseases associated with EIF3A include Bardet-Biedl Syndrome. Among its related pathways are Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S and Viral mRNA Translation. Gene Ontology (GO) annotations related to this gene include translation initiation factor activity.
Entrez Gene ID
Human8661
Rabbit100356830
UniProt ID
HumanQ14152
RabbitG1SMZ5
Alternative Names
Eukaryotic Translation Initiation Factor 3 Subunit A; Eukaryotic Translation Initiation Factor 3, Subunit 10 Theta, 150/170kDa; Eukaryotic Translation Initiation Factor 3 Subunit 10; EIF-3-Theta; EIF3 P167; EIF3 P180; EIF3 P185; EIF3S10; Eukaryotic Translation Initiation Factor 3, Subunit 10 (Theta, 150/170kD); Eukaryotic Translation Initiation Factor 3, Subunit 10 (Theta, 170kD); Eukaryotic Translation Initiation Factor 3, Subunit 10, 170kD; Eukaryotic Translation Initiation Factor 3, Subunit A; Cytoplasmic Protein P167
Research Area
RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:17581632, PubMed:25849773).

The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:17581632, PubMed:11169732).

The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773, PubMed:27462815).

(Microbial infection) Essential for the initiation of translation on type-1 viral ribosomal entry sites (IRESs), like for HCV, PV, EV71 or BEV translation (PubMed:23766293, PubMed:24357634).

(Microbial infection) In case of FCV infection, plays a role in the ribosomal termination-reinitiation event leading to the translation of VP2 (PubMed:18056426).
Biological Process
Formation of cytoplasmic translation initiation complex Source: UniProtKB
IRES-dependent viral translational initiation Source: UniProtKB
Negative regulation of ERK1 and ERK2 cascade Source: Ensembl
Translational initiation Source: UniProtKB
Translation reinitiation Source: GO_Central
Viral translational termination-reinitiation Source: UniProtKB
Cellular Location
Cytoplasm
PTM
Phosphorylated. Phosphorylation is enhanced upon serum stimulation.

Ma, S., Dong, Z., Huang, Y., Liu, J. Y., & Zhang, J. T. (2022). eIF3a regulation of mTOR signaling and translational control via HuR in cellular response to DNA damage. Oncogene, 41(17), 2431-2443.

Zhao, Y., Zhao, H., Zhang, D., Quan, Q., Ge, Y., Li, L., & Guo, L. (2022). YTHDF3 Facilitates eIF2AK2 and eIF3A Recruitment on mRNAs to Regulate Translational Processes in Oxaliplatin-Resistant Colorectal Cancer. ACS Chemical Biology, 17(7), 1778-1788.

Shu, X. E., Mao, Y., Jia, L., & Qian, S. B. (2022). Dynamic eIF3a O-GlcNAcylation controls translation reinitiation during nutrient stress. Nature Chemical Biology, 18(2), 134-141.

Mei, C., Sun, Z. E., Tan, L. M., Gong, J. P., Li, X., & Liu, Z. Q. (2022). eIF3a‐PPP2R5A‐mediated ATM/ATR dephosphorylation is essential for irinotecan‐induced DNA damage response. Cell proliferation, 55(4), e13208.

Jiang, J. S., Zang, J., Ru, Y., Luo, Y., Song, J. K., Luo, Y., ... & Li, B. (2022). Patient-driven discovery of CCN1 to rescue cutaneous wound healing in diabetes via the intracellular EIF3A/CCN1/ATG7 signaling by nanoparticle-enabled delivery. Biomaterials, 121698.

Chen, J., Liu, J. Y., Dong, Z. Z., Zou, T., Wang, Z., Shen, Y., ... & Yin, J. Y. (2021). The effect of eIF3a on anthracycline-based chemotherapy resistance by regulating DSB DNA repair. Biochemical Pharmacology, 190, 114616.

Miao, B., Wei, C., Qiao, Z., Han, W., Chai, X., Lu, J., ... & Cai, J. (2019). eIF3a mediates HIF1α-dependent glycolytic metabolism in hepatocellular carcinoma cells through translational regulation. American Journal of Cancer Research, 9(5), 1079.

Heo, C. K., Hwang, H. M., Lee, H. J., Kwak, S. S., Yoo, J. S., Yu, D. Y., ... & Cho, E. W. (2019). Serum anti-EIF3A autoantibody as a potential diagnostic marker for hepatocellular carcinoma. Scientific reports, 9(1), 1-13.

Huang, M. S., Yuan, F. Q., Gao, Y., Liu, J. Y., Chen, Y. X., Wang, C. J., ... & Liu, Z. Q. (2019). Circular RNA screening from EIF3a in lung cancer. Cancer Medicine, 8(9), 4159-4168.

Yin, J. Y., Zhang, J. T., Zhang, W., Zhou, H. H., & Liu, Z. Q. (2018). eIF3a: a new anticancer drug target in the eIF family. Cancer letters, 412, 81-87.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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