Mouse Anti-EIF3F Recombinant Antibody (CBFYE-0679) (CBMAB-E1100-FY)

Name: Mouse Anti-EIF3F Recombinant Antibody (CBFYE-0679)
SKU: CBMAB-E1100-FY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYE-0679

Application

ELISA, IF, IP, WB

Immunogen

Amino acids 179-206 within an internal region of human eIF3ε.

Host Species

Mouse

Specificity

Human, Mouse, Rat, Horse, Cattle

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
ELISA	1:100-1:1,000
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium Azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Eukaryotic Translation Initiation Factor 3 Subunit F

Introduction

EIF3F (Eukaryotic Translation Initiation Factor 3 Subunit F) is a Protein Coding gene. Among its related pathways are Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S and Viral mRNA Translation. Gene Ontology (GO) annotations related to this gene include thiol-dependent ubiquitin-specific protease activity and translation initiation factor binding. An important paralog of this gene is PSMD7.

Entrez Gene ID

Human	8665
Mouse	66085

UniProt ID

Human	O00303
Mouse	Q9DCH4

Alternative Names

Eukaryotic Translation Initiation Factor 3 Subunit F; Eukaryotic Translation Initiation Factor 3, Subunit 5 Epsilon, 47kDa; Deubiquitinating Enzyme EIF3f; EIF-3-Epsilon; EIF3S5; Eukaryotic Translation Initiation Factor 3, Subunit 5 (Epsilon, 47kD); Eukaryotic Translation Initiation Factor 3, Subunit F

Research Area

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:17581632, PubMed:25849773, PubMed:27462815).

The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:17581632).

The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773).

Deubiquitinates activated NOTCH1, promoting its nuclear import, thereby acting as a positive regulator of Notch signaling.

Biological Process

Formation of cytoplasmic translation initiation complex Source: UniProtKB-UniRule
IRES-dependent viral translational initiation Source: UniProtKB
Protein deubiquitination Source: FlyBase
Translational initiation Source: UniProtKB

Cellular Location

Cytoplasm

Involvement in disease

Intellectual developmental disorder, autosomal recessive 67 (MRT67):
A form of mental retardation, a disorder characterized by significantly below average general intellectual functioning associated with impairments in adaptive behavior and manifested during the developmental period. Some MRT67 patients manifest seizures and sensorineural hearing loss.

PTM

Phosphorylation is enhanced upon serum stimulation. Phosphorylated during apoptosis by caspase-processed CDK11.

Nicoli, E. R., Streaţa, I., Yang, J., Macnamara, E., Wolfe, L., Garcia, K., ... & Adams, D. (2022). eP198: EIF3F compound heterozygous genotype-phenotype association. Genetics in Medicine, 24(3), S123.

Lizzi, M. (2021). Investigation of the role of eukaryotic initiation factor 3f (eIF3f) and heterogeneous nuclear ribonucleoprotein K (hnRNP K) in stress induced RNA disruption in melanoma cells (Doctoral dissertation, Laurentian University of Sudbury).

Hüffmeier, U., Kraus, C., Reuter, M. S., Uebe, S., Abbott, M. A., Ahmed, S. A., ... & Zweier, C. (2021). EIF3F-related neurodevelopmental disorder: refining the phenotypic and expanding the molecular spectrum. Orphanet journal of rare diseases, 16(1), 1-9.

Andoh, T., Yoshihisa, Y., Rehman, M. U., Tabuchi, Y., & Shimizu, T. (2021). Berberine induces anti-atopic dermatitis effects through the downregulation of cutaneous EIF3F and MALT1 in NC/Nga mice with atopy-like dermatitis. Biochemical Pharmacology, 185, 114439.

Li, J., Yu, W., Ge, J., Zhang, J., Wang, Y., Wang, P., & Shi, G. (2020). Targeting eIF3f Suppresses the Growth of Prostate Cancer Cells by Inhibiting Akt Signaling. OncoTargets and therapy, 13, 3739.

Docquier, A., Pavlin, L., Raibon, A., Bertrand‐Gaday, C., Sar, C., Leibovitch, S., ... & Bernardi, H. (2019). eIF3f depletion impedes mouse embryonic development, reduces adult skeletal muscle mass and amplifies muscle loss during disuse. The Journal of Physiology, 597(12), 3107-3131.

ChaoHui, L., ZhenYu, Z., Ling, X., HuiLing, R., & XiaoJun, W. (2019). Interaction between influenza A virus NS1 protein and host protein eIF3f promotes viral replication. Zhongguo Yufang Shouyi Xuebao/Chinese Journal of Preventive Veterinary Medicine, 41(10), 999-1003.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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